In Vitro Culture of Turkish Origanum sipyleum L.
American Journal of Plant Biology
Volume 2, Issue 3-1, September 2017, Pages: 32-36
Received: Apr. 20, 2017; Accepted: Apr. 21, 2017; Published: Jul. 17, 2017
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Başar Sevindik, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Tolga İzgü, Department of Horticulture, Faculty of Agriculture, University of Ege Bornova, İzmir, Turkey
Özhan Şimşek, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Mehmet Tütüncü, Department of Horticulture, Faculty of Agriculture, University of Ondokuz Mayıs Atakum, Samsun, Turkey
Pembe Çürük, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Özer Yılmaz, Department of Biology, Faculty of Arts and Sciences, University of Uludağ, Nilüfer, Bursa, Turkey
Gönül Kaynak, Department of Biology, Faculty of Arts and Sciences, University of Uludağ, Nilüfer, Bursa, Turkey
Yıldız Aka Kaçar, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
Jaime A. Teixeira da Silva, Miki-cho Post Office, Ikenobe, Kagawa-ken, Japan
Yeşim Yalçın Mendi, Department of Horticulture, Faculty of Agriculture, University of Çukurova Balcalı, Adana, Turkey
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Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.
BA, GA3, IBA, MS Medium, Tissue Culture
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Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, Özer Yılmaz, Gönül Kaynak, Yıldız Aka Kaçar, Jaime A. Teixeira da Silva, Yeşim Yalçın Mendi, In Vitro Culture of Turkish Origanum sipyleum L., American Journal of Plant Biology. Special Issue: Plant Molecular Biology and Biotechnology. Vol. 2, No. 3-1, 2017, pp. 32-36. doi: 10.11648/j.ajpb.s.2017020301.16
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