An Overview of In Vitro Haploid Plant Production in Citrus
American Journal of Plant Biology
Volume 2, Issue 3-1, September 2017, Pages: 19-23
Received: Feb. 24, 2017; Accepted: Feb. 25, 2017; Published: Mar. 20, 2017
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Mehmet Akgol, Institute of Natural and Applied Sciences, Department of Biotechnology, Cukurova University, Adana, Turkey
Ozhan Simsek, Faculty of Agriculture, Department of Horticulture, Faculty of Agriculture, Cukurova University, Adana, Turkey
Dicle Donmez, Institute of Natural and Applied Sciences, Department of Biotechnology, Cukurova University, Adana, Turkey
Yildiz Aka Kacar, Institute of Natural and Applied Sciences, Department of Biotechnology, Cukurova University, Adana, Turkey; Faculty of Agriculture, Department of Horticulture, Faculty of Agriculture, Cukurova University, Adana, Turkey
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The main objectives of Citrus breeding are to have new varieties with a shorter juvenile non-fruiting period, an increased yield, a longer ripening season, regular fruit bearing, seedlessness and improved external and internal quality of the fruits. To make available new scions and rootstocks selected for resistance or tolerance to biotic and abiotic stresses is another important aim in Citrus improvement. Citrus breeding is based either on conventional methods (hybridization, selection, mutation) or biotechnological methods employing in vitro tissue culture, regeneration from protoplasts, somatic hybridization, in vitro mutant selection, genetic transformation and haploid production. An integrated approach between innovative and conventional tools is fundamental to obtaining large improvements in a short time. Haploid plants have some advantages for the plant breeding because of their one set of chromosomes, identified recessive mutations and reducing the breeding time. There are several methods to produce haploid plants such as androgenesis and gynogenesis. The pollen and microsphore cultures are used for androgenesis. The ovule and ovary culture are used for the gynogenesis. In this review we describe the principals of the haploid plant cultures and we illustrated some haploid studies in citrus.
Haploidy, Androgenesis, Gynogenesis, Anther and Microsphore Culture, Ovule and Ovary Culture
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Mehmet Akgol, Ozhan Simsek, Dicle Donmez, Yildiz Aka Kacar, An Overview of In Vitro Haploid Plant Production in Citrus, American Journal of Plant Biology. Special Issue: Plant Molecular Biology and Biotechnology. Vol. 2, No. 3-1, 2017, pp. 19-23. doi: 10.11648/j.ajpb.s.2017020301.13
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