Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages
American Journal of Plant Biology
Volume 3, Issue 2, June 2018, Pages: 21-28
Received: Aug. 22, 2018;
Accepted: Sep. 19, 2018;
Published: Oct. 10, 2018
Views 1014 Downloads 106
Rongfa Chen, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Xing Huang, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Lihang Qiu, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Yegeng Fan, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Ronghua Zhang, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Jinlan Xie, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Jianming Wu, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China
Yangrui Li, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, China; Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Nanning, China
Sugarcane is the most important sugar crop in China, which mainly focuses on the upper stem of the harvested land. The proper regulation of the proportion of internode elongation is the key to determine the yield and sugar. Therefore, it is of great significance to study the mechanism of the dynamic change of elongation between cane joints in order to improve the yield of sugar cane and sucrose. To investigate the biochemical mechanism of stem elongation in sugarcane, stem samples were collected at the pre-elongation stage (9-10 leaves) (Ls1), early elongation stage (12-13 leaves) (Ls2) and rapid elongation stage (15-16 leaves) (Ls3). The change trends in the activities of NAD kinase (NADK), calcium-dependent protein kinase (CDPKs), α-mannosidase, α-galactosidase, β-glucosidase, cellulase, xyloglucan endo-transglycosylase/hydrolase (XTH) and catalase (CAT) were completely consistent, showing rapid elongation stage > early elongation stage > pre-elongation stage, while the activities of β-glucosidase, peroxidase (POD) and α-glucosidase were in opposite, and the activities of calmodulin and β-mannosidase showed the same single-peak trend, and the peak was at early elongation stage. The enzyme activities of β-galactosidase and pectinase did not show significant difference at different stages. The results indicate that the elongation of internodes was closely related to the complex physiological metabolism of sugarcane, and the key enzymes play roles at different time but β-galactosidase and pectinase have little effect on internodes elongation in sugarcane.
Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages, American Journal of Plant Biology.
Vol. 3, No. 2,
2018, pp. 21-28.
Li, X., Z. H. Guo (2014). A Pilot Study on Internode Elongation in a Paleotropical Bamboo，Dendrocalamus latiflorus (Poaceae: Bambusoideae). Plant Diversity and Resources 36(1):22-28.
Wang, H. Y., K. Cui , C. Y. He, Y. F. Zeng, S. X. Liao, and J. G. Zhang (2015). Endogenous hormonal equilibrium linked to bamboo culm development. Genetics Molecular Research 14(3):11312-11323.
He, C. Y., K. Cui, J. G. Zhang, A. G. Duan, and Y. F. Zeng (2013). Next-generation sequencing-based mRNA and microRNA expression profiling analysis revealed pathways involved in the rapid growth of developing culms in Moso bamboo. BMC Plant Biology 13(1):1-14.
Peng, Z. H., CH. L. Zhang, Y. Zhang, T. Hu, S. H. Mu, X. P. Li, and J. Gao (2013). Transcriptome sequencing and analysis of the fast growing shoots of Moso bamboo(Phyllostachys edulis). PLOS One 8(11):e78944.
Gao, J., Y. Zhang, C. L. Zhang, F. Y. Qi, X. P. Li, SH. H. Mu, and ZH. H. Peng (2014). Characterization of the floral transcriptome of Moso bamboo (Phyllostachys edulis) at different flowering developmental stage by transcriptome sequencing and RNA-seq analysis. PLOS One 9(6): e98910.
Zhang, Y., G. Tian, H. P. Lu, R. Hong, T. Hu, and Q. R. Guo.2015. Transcriptome characterization of phyllostachys edulis‘Pachyloen’shoots in different solar terms. Acta Agriculturae Universitatis Jiangxiensis 37(3):466-474.
Zhou, M. B., Y. Zheng, L. Z. Liu, X. W. Xia, D. Q. Tang, Y. Fu, and M. Chen (2016). Endo-1,4-β-glucanase gene involved into the rapid elongation of Phyllostachys heterocycla var. pubescens. Trees 30(4):1259-1274.
Cui, K., C. Y. He, J. G. Zhang, A. G. Duan, and Y. F. Zeng (2012). Temporal and spatial profiling of Internode elongation associated protein expression in rapidly growing culms of bamboo. Journal of Proteome Research 11: 2492–2507.
Li, P (2012). DNA methylation analysis and QTL mapping for internode elongation of Maize (Zea mays L.). Shijiazhuang, Hebei: Agricultural University of Hebei.
Zhang, K (2014). Methylation and expression analysis of genes involved in the Internode elongation in Maize. Wuhan, Hubei: Huazhong Agricultural University.
Ma, L. G., and D. Y. Sun (1996). The extracellulr reaction sites of calmodulin on pollen and pollen tubes of Hippeastrumrurilum. Progress in Natural Science (6):505-509.
Cui, S. J., H. H. Wang, L. G. Ma, and D. Y. Sun (1998). The effects of extracellular calmodulin of style and pollen on pollen germination and pollen tube growth. Plant Physiology Journal 24(4):320-326.
Yang, H. Y (1999). The role of calcium in the fertilization process in flowering plants. Chinese Bulletin of Botany 41(10):1027-1035.
Allan, E., and A. Trewavas (1985). Quantitative changes in calmodulin and NAD kinase during early cell development in the root apex of Pisum sativum L. Planta 165:493-501.
Wang, X. J., and R. Z. Pan (1993). Effects of red light on calcium accumulation, activities of ATPase and NAD kinase in mitochondria isolated from hypocotyls segments of Mung Bean. Plant Physiology Journal 19(1):71-76.
Liu, G. S., and J. Chen (2003). Roles of calcium_dependent protein kinases (CDPKs) in plant calcium signal transduction. Chinese Bulletin of Botany 20:160-197.
Huang, J. Z., S. C. Hardin, and S. C. Huber (2001). Identification of a novel phosphorylation motif for CDPKs: Phosphorylation of synthetic peptides lacking basic residues at P-3/P-4. Archives of Biochemistry Biophysics 393:61-66.
Breviario, D., L. Morello, and S. Giani (1995). Molecular cloning of two novel rice cDNA sequences encoding putative calcium- dependent protein kinase. Plant Molecular Biology 27(5):953- 967.
Li, L. C., X. C. Wang (1998). The relationship between plant cell elongation and wall properties under water deficits. Plant Physiology Communications 34(3):161- 167(in Chinese).
Li, L. C., X. C. Wang. 1996. Effects of water deficit on plant cell wall and its relation to cell elongation. Plant Physiology Communications 32(5):321-327.
Hayashi, T., Y. S. Wong, and G. Maclachlan (1984). Pea xyloglucan and cellulose:Ⅱ. Hydrolysis by pea endo-1,4-glucanases. Plant Physiology 75:605-610.
Xuan, Y., H. F. Zhao, X. Y. Guo, J. Ren, Y. Wang, and B. Y. Lu (2016). Plant cell wall remodeling enzyme xyloglucan endotransglucosylase/hydrolase (XTH). Chinese Agricultural Science Bulletin 32(8):83-88.
Fry, S. C. (2000). The Growing Plant Cell Wall: Chemical and Metabolic Analysis. New Jersey: The Blackburn Press, Caldwell.
Cosgrove, D. J (2005). Growth of the plant cell wall. Nature Reviews Molecular Cell Biology 6:850-861.
Potter, I., and S. C. Fry (1993). Xyloglucan endotransglycosylase activity in pea internodes. Plant Physiology 103(1):235-241.
Potter, I., and S. C. Fry (1994). Changes in xyloglucan endotransglycosylase (XET) activity during hormone- induced growth in lettuce and cucumber hypocotyls and spinach cell suspension cultures. Journal of Experimental Botany 45:1703-1710.
Smith, R. C., and P. R. Matthews (1996). The regulation of leaf elongation and xyloglucan endotransglycosylase by gibberellin in ‘Himalaya’ barley (Hordeum vulgare L). Journal of Experimental Botany 47(302):1395-1404.
Michailidis, G, A. Argiriou, N. Darzentas, and A. Tsaftaris (2009). Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes from allotetraploid (Gossypium hirsutum) cotton and its diploid progenitors expressed during fiber elongation. Journal of Plant Physiology 166(4): 403-416.
Jimenez, T., I. Martin, E. Labrador, and B. Dopico (2006). The immunolocation of a xyloglucan endotransglucosylase/hydrolase specific to elongating tissues in Cicer arietinum suggests a role in the elongation of vascular cells. Journal of Experimental Botany 57(15): 3979-3988.
Hernandez, N. J., I. Martin, E. Labrador, and B. Dopico (2010). The immunolocation of XTH1 in embryonic axes during chickpea germination and seedling growth confirms its function in cell elongation and vascular differentiation. Journal of Experimental Botany 61(15): 4231-4238.
Hyodo, H., S. Yamakawa, Y. Takeda, M. Tsuduki, A. Yokota, K. Nishitani, and T. Kohchi (2003). Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in infolorescence apices is related to cell elongation in Arabidopsis thaliana. Plant Molecular Biology 52(2):473-482.
Shin, Y. K., H. K. Yum, E. S. Kim, H. Cho, K. M. Gothandam, J. Hyun, and Y. Y. Chung (2006). BcXTH1, a Brassica campestris homologue of arabidopsis XTH9, is associated with cell expansion. Planta 224(1):32-41.
Yokoyama, R., J. K. C. Rose, and K. Nishitani (2004). A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis. Plant Physiology 134(3): 1088-1099.
He, H., R. Serraj, and Q. Yang (2009). Changes in Os XTH gene expression, ABA content, and peduncle elongation in rice subjected to drought at the reproductive stage. Acta Physiologiae Plantarum 31(4): 749-756.
Schopfer, P (1996). Hydrogen peroxide-mediated cell-wall stiffening in vitro in maize coleoptiles. Planta 199:43-49.
Fry, S. C (1998). Oxidation scission of plant cell wall polysaccharides by ascorbate induced hydroxyl radicals. Biochemical Journal 332:507–515.
Neill, S. J., R. Desikan, A. Clarke, R. D. Hurst, and J. T. Hancock (2002). Hydrogen peroxide and nitric oxide as signaling molecules in plants. Journal of Experimental Botany 53(372):1237–1247.
Drążkiewicz, M., E. Skórzyńska-Polit, and Z. Krupa (2004). Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana. Bio Metals 17(4):379–387.
Wu, J. M., Y. R. Li, A. Q. Wang, Y. Yang, and L. T. Yang (2010). Relationship between gibberellin-induced internode elongation and related enzyme activities in sugarcane. Acta Botanica Boreall-Occidentalla Sinica 30(5):0962-0967.
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Science Research 14(2):93–107.
Intapruk, C., K. Yamamoto, M. Sekine, M. Takano, and A. Shinmyo (1994). Regulatory sequences involved in the peroxidase gene expression in Arabidopsis Thaliana. Plant Cell Reports 13(3):123-129.
Liu, Z. H., and M. J. Ger (1997). Changes of enzyme activity during pollen germination in maize, and possible evidence of lignin synthesis. Functional Plant physiology 24(3):329-335.
Liang, Y. R., X. H. H, Y. L. Zhang, and X. P. Liu (2003). Prdgress on physiological function research of plant preoxidase. Journal of Inner Mongolia Agricultural University 24(2):110-113.
Zhou, R. G, Y. S. Han, L. F. Yan (1983). The relationship between plant height and peroxidase. Acta Agronomica Sninica 9(4):267-273.
Liang, G. H., K. C. Lee, K. Chung, Y. T. Liang, and B. A. Cunningham (1977). Regulation of internnode length by peroxidase enzymes in grain sorghum. Theoretical and Applied Genetics 50(3):137-146.