Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling
American Journal of Plant Biology
Volume 3, Issue 2, June 2018, Pages: 17-20
Received: Aug. 22, 2018;
Accepted: Sep. 12, 2018;
Published: Oct. 10, 2018
Views 790 Downloads 58
Wenjing Huang, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Guangzhu Yang, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Fan Li, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Liping Zheng, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Jun Ma, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Follow on us
Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.
LED Apple Root-Stock Tissue Culture Seedling Growth and Development
To cite this article
Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling, American Journal of Plant Biology.
Vol. 3, No. 2,
2018, pp. 17-20.
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Briand CH, Hicks GS (1989). Micropropagation of the cold-hardy apple rootstock KSC-3: a morphological analysis. Can J Plant Sci, 69: 555-564
Kermani MJ, Hosseini ZS, Habashi AA (2009). A refined tissue culture medium for in vitro proliferation of apple roots-tocks. Acta Hort (ISHS), 829: 313-318
Sicurani M, Piccioni E, Standardi A (2001). Micropropagation and preparation of synthetic seed in M. 26 apple rootstock I: attempts towards saving labor in the production of adventitious shoot tips suitable for encapsulation. Plant Cell Tiss Organ Cult, 66: 207-216
Yaseen M, Ahmad T, Abbasi NA, Hafiz IA (2009). Assessment of apple rootstocks M9 and M26 for in vitro rooting potential using different carbon sources. Pak J Bot, 41 (2): 769-781
Wenke Liu, Qichang Yang, lingling Wei, LED light source and its horticultural application[M], Agricultural Science and Technology Press of China , 2012
Tissue culture of Apple Dwarf rootstock 'JM7' and its adventitious shoot regeneration in vitro, Plant Physiology Journal 2014, 50 (6): 779-78
Liu X. Y., Chang T. T., Guo S. R., et al. Effect of different light quality of LED on growth and photosynthetic character in cherry tomato seedling. Acta Horticulturae, 2011, 907: 325-330
Yanagi T., Okamoto K., Takita S. Effect of blue and red light intensity on photosynthetic rate of strawberry leaves. Acta Horticultruae, 1996, 440: 371-376
Jao R. C., Lai C. C., Fang W., et al. Effects of red light on the growth of Zantedeschia plant lets in vitro and tuber formation using light-emitting diodes. HorScience, 2005, 40(2): 436-438
Wenjing Huang, Guangzhu Yang, Liping Zheng, Jun Ma, Effects of different light quality on growth and development of Pitaya tissue culture seedlings, China Fruits, 2014, (4)
Rueychi J., Wei F. Growth of potato plantlets in vitro is different when provided concurrent versus alternating blue and red light photoperiods. HortScience, 2004, 39(2): 380-382