Please enter verification code
Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats
International Journal of Bioorganic Chemistry
Volume 4, Issue 1, June 2019, Pages: 19-23
Received: Feb. 8, 2019; Accepted: Mar. 8, 2019; Published: Mar. 30, 2019
Views 809      Downloads 96
Gehan Fawzy Abdel Raoof, Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt
Khaled Younes Mohamed, Internal Medicine Department, Medical Division, National Research Centre, Giza, Egypt
Emam Waked, Nephrology Department, Theodor Bilharz Research Institute, Cairo, Egypt
Hesham Anwar Gomaa, Biochemistry Department, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt; Pharmacology Department, College of Pharmacy, Jouf University, Sakakah, Saudi Arabia
Article Tools
Follow on us
The present work was carried out to studytheprotective effect of 70% methanolic extract of Diospyros lotus L. aerial parts against hepato and nephro-toxicity that was induced bycarbon tetrachloride. In addition, phytochemical screening, total phenolic, total flavonoid contents and proximate analysisof the aerial parts were determined. Methanol (70%) extracts of the aerial parts of Diospyros lotus L. was prepared. Two main groups of rats were assigned which were normal and damaged liver groups that administrated CCl4 (carbon tetra chloride). Each group classified into 4 different groups normalcontrol: was received distilled water, positive control: was administered silymarin (50 mg/kg), and treated groups:were administered Diospyros lotus tested extract at two dose levels (150 and 300 mg/kg) for 15 days. Serum alanine and aspartate aminotransferase activities were determined to evaluate hepatoprptective effect, while uric acid and creatinine levels were measured to study renal function protective effects. The results showed that the alterations induced by carbon tetrachloride were significantly restored by methanol extract of Diospyros lotus (150 and 300 mg/kg) in dose dependant manner. Alkaloids, carbohydrates, tannins, flavonoids, saponins and unsaturated sterols and/or triterpenes were detected as the main active constituents of the methanol extract. It can be concluded that the 70% methanol extract of Diospyros lotus L. aerial parts has a significant protective effect against acute hepato-toxicity and nephro-toxicity induced by CCl4 in ratsso it can be used as a safe hepato-nephro protective drug.
Carbon Tetrachloride, Diospyros Lotus, Hepatotoprotective, Nephro-Toxicity
To cite this article
Gehan Fawzy Abdel Raoof, Khaled Younes Mohamed, Emam Waked, Hesham Anwar Gomaa, Phytochemical Study and Protective effect of Diospyros lotus Against Carbon Tetrachloride-Induced Hepato and Nephro-Toxicity in Rats, International Journal of Bioorganic Chemistry. Vol. 4, No. 1, 2019, pp. 19-23. doi: 10.11648/j.ijbc.20190401.14
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ahsan, R., Monirul Islam, K. M., Musaddik, A., Hague, E., (2009). Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride induced hepatotoxicity in albino rats. Glob J Pharmacol, 3(3): 116-122.
Nazeema, T. H., Brindha, V., (2009). Antihepatotoxic and antioxidant defense potential of Mimosa pudica. Int J Drug Discov, 1: 1-4.
Manokaran, Jaswanth, A., Sengottuvelu, S., Nandhakumar, J., Duraisamy, R., Karthikeyan, D., et al. (2008). Hepatoprotective activity of Aervalanata Linn. againstparacetamol induced hepatotoxicity in rats. Res J Pharm Tech, 1(4): 398-400.
Absar, A., Quershi, Prakash T et al. (2007). Hepatoprotective and Antioxidant activities of flowers of Calotropisprocera (Ait) r. Br. in CCl4 induced hepatic damage. Indian J Exp Biology, 45:304-310.
Ajith, T. A., Janardhanan, K. K., (2002). Antioxidant and antihepatotoxicactivites of Phellinusrimosus (Berk) Pilat. J Ethanopharmacol, 81:387-391.
Mohameed, S. T. S., Christina A. J. M., Chidambaranathan, N., (2008)Hepatoprotective activity of Annonasquamosa Linn. on experimental animal model. Int J App Res Nat Pro, 1(3):1-7.
Uddin, G., Rehman, T. U., Arfan, M., Liaqat, W., Waliullah, et al. (2011) Antimicrobial, insecticidal and phytotoxic activities of Indigoferaheterantha roots. J Med Plants Res, 5: 5835-5839.
Pant, S., Samant, S. S., (2010). Ethanobotanical observation in the Momaula Reserve Forest of KoumounWest Himalaya, India. Ethnobotanical Leaflets 1493.
Watt, J. M., Breyer-Brandwijk, M. G., (1932) The Medicinal and Poisonous Plants of South Africa. Postgrad Med J 8: 427.
Simmons, A. E., (1972). Growing Unusual Fruit. David and Charles, NewYork, ISBN: 0-7153-5531-7.
Chopra, R. N., Nayar, S. L., Chopra, I. C., (1986). Glossary of Indian Medicinal Plants (Including the Supplement). I stEdn., Council of Scientific and Industrial Research, New Delhi.
Ebrahimzadeh, M. A., Pourmorad, F., Bekhadnia, A. R., (2008). Iron chelating activity screening phenol and flavonoid content of some medicinal plants from Iran. African Journal of Biotechnology 7, 3188-3192.
Bown, D., (1995). Encyclopaedia of Herbs and Their Uses. Dorling Kindersely, Ltd., London, ISBN: 07513-020-31. pp 342.
Ahmet, F. A., Kadioglu, A., (1998). Non volatile acid composition during fruits development of Diospyros lotus L. Turkish Journal of Botany 22, 69-72.
Khasan, T., Matyukhina, L. G., Saltykova, I. A., (1976). Triterpenoids of Diospyros lotus. Chemistry of Natural compounds 11, 118-123.
Yoshihira, K., Tezuka, M., Natori, S., (1971). Naphthoquinones derivatives from the Abenaceae. II. Isodiospyrin, bisisodiospyrin and mamegakinone from Diospyros lotus L and D. morrisianaHance. Chemical Pharmaceutical Bulletin 19, 2308-2313.
Khaled, R., Maria, C. B. (2017). Biological Activities of Plants used in Egyptian Ethnopharmacology. Journal of Applied Pharmaceutical Science 7 (05): 046-050.
Harbone, J. B., (1973). Phytochemical methods. London: Chapman and Hall, Ltd.; p 49.
Sofowora, A., (1993)Medicinal plants and Traditional medicine in Africa., Ibadan, Nigeria: Spectrum Books Ltd, p 289.
Siger A., Nogala-Kalucka M., Lampart-Szczapa E., (2008). The content and antioxidant activity of phenolics compounds in cold-pressed plant oils. J Food Lipids 15: 137 –149.
Zhishen, J., Mengcheng, T., Jianming, W., (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555 – 559.
Egyptian Pharmacopoeia, (1984). General Organization for Governmental Printing Office, Ministry of Health, Cairo, Egypt, 31-33.
Karber, (1931). Quantal Responses. Calculation of ED50. In: Turner RA. editor. Screening Methods in Pharmacology. New York and London: Acadmic Press; p. 63, 64.
Cocchetto, D. M., Bjornsson, T. D., (1983). Methods for vascular access and collection of body fluids from the laboratory rat. J Pharm Sci, 72:465-492.
Reitman, S., Frankel, S., (1957). Colorimetric method for aspartate and alanine transferases. Am J ClinPathol, 28:56–63.
Barham, D., Trinder, P., (1972). An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst, 97:142-145.
Bartles, H., Bohmer, M., Heirli, C., (1972). Serum creatinine determination without protein precipitation. ClinChem Acta, 37:193-197.
Hertog, M. L., Feskens, E. J., Hollman, P. H., Katan, M. B., Kromhout, D., (1993). Dietary antioxidants flavonoids and the risk of coronary heartdisease: the zutphen elderly study. Lancet, 342: 1007-1011.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186