Preliminary Antifungal Study of Some Essential Oils of Three Medicinal Plants Against Fusarium Oxysporum f. sp. ciceris, and Aspergillus Niger
International Journal of Bioorganic Chemistry
Volume 3, Issue 1, June 2018, Pages: 1-5
Received: Aug. 17, 2017; Accepted: Nov. 9, 2017; Published: Jan. 9, 2018
Views 1710      Downloads 198
Farah Khameis Farag Teia, Department of Agro-technology, Medicinal and Aromatic Plants and Traditional Medicine Research Institute, National Centre for Research, Khartoum, Sudan
Ahmed Osman, Department of Microbiology, Medicinal and Aromatic Plants and Traditional Medicine Research Institute, National Centre for Research, Khartoum, Sudan
Magda Abaker Osman, Department of Agro-technology, Medicinal and Aromatic Plants and Traditional Medicine Research Institute, National Centre for Research, Khartoum, Sudan
Article Tools
Follow on us
The aim of this study is to investigate preliminary antifungal activity of three essential oils by disc diffusion method, the essential oils of three plants used in traditional Sudanese medicine namely Mentha spicata (Lamiaceae), Cymbopogon schoenanthus L. (Poaceae), and Citrus reticulata (Rutaceae), was evaluated for their ability to inhibit the growth of selected phytopathogenic fungi Fusarium oxysporum f. sp. ciceris (causing wilt in chick pea) and the standard fungi Aspergillus niger was used for comparison. Essential oils were extracted by hydrodistilation using Clevenger apparatus. The experiment was carried out using disc diffusion method in different concentrations of essential oil on PDA culture at 25°C. The result showed essential oil of studied plants in all concentrations had completely inhibited growth for tested fungi.
Antifungal, Essential Oil, Disc Diffusion, Phytopathogen
To cite this article
Farah Khameis Farag Teia, Ahmed Osman, Magda Abaker Osman, Preliminary Antifungal Study of Some Essential Oils of Three Medicinal Plants Against Fusarium Oxysporum f. sp. ciceris, and Aspergillus Niger, International Journal of Bioorganic Chemistry. Vol. 3, No. 1, 2018, pp. 1-5. doi: 10.11648/j.ijbc.20180301.11
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ozcan, M. M. L., O. Sagdic and Ozkan, G. (2006). Inhibitory effects of spice essential oils on the growth of Bacillus species. J. Med. Food, 9: 418-421.
Chee, H. Y., and Lee, M. H. (2007). Antifungal activity of clove oil and its volatile vapour against dermatophytic fungi. Mycology, 35: 241-243.
Andrea Bernardos., Teresa Marina., Petr Žáček., Édgar Pérez-Esteve., Ramón Martínez-Mañez., Miloslav Lhotka., Lenka Kouřimská., Josef Pulkrábek and Pavel Klouček. (2015). Antifungal effect of essential oil components against Aspergillus nigerwhen loaded into silica mesoporous supports. Journal of the Science of Food and Agriculture, 95 (14): 2824–2831.
Khan, M. T., A. Ather, K. D., Thompson and Gambari, R. (2005). Extracts and molecules from medicinal plants against Herpes simplex viruses. Antivir. Rev., 67: 107-19.
Simin, N., Seyyed Alireza Esmaeilzadeh Hosseini, Abolfzl Sarpeleh, Mahmoud SoflaeiShahrbabak and Yeganeh SoflaeiShahrbabak (2011). Antifungal Activity of Spearmint (Mentha Spicata L.) Essential Oil on Fusarium oxysporum f. sp. radicis-cucumerinum the Causal Agent of Stem and Crown Rot of Greenhouse Cucumber in Yazd, Iran. IPCBEE vol. 15: 54.
Ibrahim, A. M., Kainulainen, P., Aflatuni, A., Tiilikkala, K., Holopainen, J. (2001). Insecticidal, repellent, antimicrobial activity and phytotoxicity of essential oils: With special reference to limonene and its suitability for control of insect pests. Agricultural and Food Science in Finland, 10: 243–59.
Aflatuni, A. (2003). The use of plant origin substances against Galerucella sagitaria. Proceedings of the Nordic Association of Agricultural Scientists 22nd Congress “Nordic Agriculture in Global. Perspective”, Turku, Finland.
Bruneton, J. (1995). Pharmacognosy, Phytochemistry, Medicinal Plants. Lavoisier Publ Londres, New York, Paris. PP: 405–66.
Lahlou, M. (2004). Essential oils and fragrance compounds: bioactivity and mechanisms of action. Flavour and Fragrance Journal, 19: 159–165.
Hadjlaoui, H., Najla, T., Emira, N., Mejdi, S., Hanen, F., Riadh, K., Amina, B. (2009). World J. Biotechnol. Microbiol. 25, 2227–2238.
PDR for Herbal Medicines, 2004. Third Edition, Thomson PDR at Montvale, 283-284.
Mimica-Dukić, N. and Božin, B. (2008). Mentha L. Species (Lamiaceae) as promising sources of bioactive secondary metabolites, Curr. Pharm. Design. 14: 3141-3150.
Khanuja, S. P. S., Shasany, A. K., Pawar, A., Lal, R. K., Darokar, M. P., Naqvi, A. A., Rajkumar, S., Sundaresan, V., Lal, N., Kumar, S. (2005). Essential oil constituents and RAPD markers to establish species relationship in Cymbopogon Spreng. (Poaceae). Biochem. Syst. Ecol., 33: 171–186.
Noudogbessi, J. P., Alitonou, G. A., Djenontin, T. and Avlessi, F. (2013). Chemical Compositions and Physico-chemical Properties of Three Varieties Essential oils of Cymbopogon giganteus Growing to the Spontaneous State in Benin. Orient. J. Chem., 29 (1): 59-67.
Boulos, L. (1999). In: Flora of Egypt, vol. 1. Al Hadara Publishing, Cairo, Egypt, pp. 291–301.
Xiangtao, C., Ke, Y., Hualiang, L. (2010). Phenolic contents and antioxidant activities in ethanol extracts of Citrus reticulata Blanco cv. Ougan fruit. Journal: Food, Agriculture and Environment (JFAE), 8 (2): 150-155.
Feás, X., Estevinho, L. M., Carmen, S., Pilar, V., Sainz, M. J., Vázquez-Tato M. P. (2013). Triacylglyceride, antioxidant and antimicrobial features of Virgin Camellia oleifera, C. reticulata and C. sasanqua Oils. Molecules, 18 (4): 4573-4587.
Phetkul, U., Nutthakran, W., Wilawan, M., Souwalak, P., Carroll, A. R., (2013). New acridone from the wood of Citrus reticulata Blanco. Natural Product Research, 27 (20): 1922-1926.
Jun, L., Teng, X., Yun-Hai, L., Shi-Zhong, W. (2012). A new limonoid from the seeds of Citrus reticulata Blanco. Natural Product Research, 26 (8): 756-761.
Khalil AT, Galal TM, El Sayed AK, (2003). Limonoids from Citrus reticulata. Zeit. Naturforsch C 2003; 58 (3): 165- 170.
Sun Y, Jianhua W, Shubo G, Zhengbo L, Yujie Z, Xiaoxia Z, (2010). Simultaneous determination of flavonoids in different parts of Citrus reticulata ‘Chachi’ fruit by high performance liquid chromatography-photodiode array detection. Molecules, 15 (8): 5378-5388.
Baayen R. P., O'donnell K., Bonants P. J. M., Cigelnik E., Kroon Laurens P. N. M., Roebroeck E. J. A. and Waalwijk C. (2000) Gene Genealogies and AFLP Analyses in the Fusarium oxysporum Complex Identify Monophyletic and Nonmonophyletic Formae Speciales Causing Wilt and Rot Disease. Phytopathology. 90: 891-900.
Haware MP, Nene YL, Rajeshwari R (1978) Eradication of Fusarium oxysporum f. sp. ciceri transmitted in chickpea seed. Phytopathology 68: 1364–1367.
Haware MP, Nene YL, Natrajan M (1986) Survival of Fusarium oxysporum f. sp. ciceri in soil in the absence of chickpea. National seminar on management of soil-borne diseases of crop plants (abstract). TNAU Coimbatore, 8–10 January.
Nene, Y. L., V. K. Sheila and S. B. Sharma. (1984). A world list of chickpea (Cicer arietinum L.) and pigeonpea (Cajanus cajan (L.) Millsp.) pathogens. ICRISAT Pulse Pathology Progress Report 32, pp. 19.
Kaiser, W. J., A. R. Alcalá-Jiménez, A. Hervas-Vargas, J. L. Trapero-Casas and R. M. Jiménez-Díaz. (1994). Screening of wild Cicer species for resistance to race 0 and 5 of Fusarium oxysporum f. sp. ciceris. Plant Disease, 78: 962-967.
National Committee for Clinical Laboratory Stan-dards (NCCLS 1999). Performance standards for antimicrobial susceptibility testing; ninth infor-mational supplement. Wayne, Pensilvaniadocu-ment M100-S9, Vol. 19.
Duru, M. E., Cakir, A., Kordali, S., Zengin, H., Harmandar, M., Izumi, S., Hirata, T. (2003). Chemical composition and Antifungal properties of Essential Oil of three Pistacia species. Fitoterapia, 74: 170-176.
Mukhtar, S. and Ghori, I. (2012). Antibacterial Activity of Aqueous and Ethanolic Extracts of Garlic, Cinnamon and Turmeric against Escherichia coli ATCC 25922 And Bacillus subtilis Dsm 3256. International Journal of Applied Biology and Pharmaceutical Technology, 3 (2): 131-136.
Jalali BL, Chand H (1992) Chickpea wilt. In: Singh US, Mukhopadhayay AN, Kumar J, Chaube HS (eds) Plant diseases of international importance, vol 1. Diseases of cereals and pulses. Prentice Hall, Englewood Cliffs, N. Y., pp 429–444.
El-Assiuty, E. M., Bekheet, F. M., Fahmy, Z. M., Ismael, A. M. and El-Alfy, T. S. M. (2006). Potentiality of some isolated compounds from Halfa barr Cymbopogon proximus Stapf. Against the toxigenic fungi Fusarium verticilliodes and Aspergillus flavus. Egypt. J. Phytopathol., 342: 75-84.
Chutia, M., P. Deka, Bhuyan, M. G. Pathak, T. C. Sarma and P. Boruah (2009). Antifungal activity and chemical composition of Citrus reticulata Blanco essential oil against phytopathogens from North East India. LWT - Food Science and Technology. 42: 777–780).
Zohra, H. F., Rachida, A., Malika, M., Benali, S., Smir, A. A. and Bourai, M. (2015). Chemical Composition and Antifungal Activity of Essential Oils of Algerian Citrus. African Journal of Biotechnology, 14 (12): 1048-1055.
Amini M, Safaie N, Salmani MJ, And Shams-Bakhsh M. (2012). Antifungal activity of three medicinal plant essential oils against some phytopathogenic fungi. Trakia J Sci, 10: 1–8.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186