Interactions of Photosynthetic Reaction Centers with Bc1 Complexes from Rhodobacter Sphaeroides Studied Using SEIRAS on a Nano-Structured Gold Surface
International Journal of Bioorganic Chemistry
Volume 2, Issue 2, June 2017, Pages: 61-69
Received: Jan. 29, 2017; Accepted: Feb. 21, 2017; Published: Mar. 22, 2017
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Authors
Vedran Nedelkovski, Austrian Institute of Technology GmbH, Vienna, Austria
Andreas Schwaighofer, Austrian Institute of Technology GmbH, Vienna, Austria; Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria
Andreas F. Geiss, Austrian Institute of Technology GmbH, Vienna, Austria
Christina Bliem, Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria; Center of Electrochemical Surface Technology, Wiener Neustadt, Austria
Renate L. C. Naumann, Austrian Institute of Technology GmbH, Vienna, Austria
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Abstract
Inter-protein reactions of photosynthetic reaction centers (RCs) with bc1 complexes from R. sphaeroides, have been investigated using surface-enhanced Infrared-absorption spectroscopy (SEIRAS). Surface enhancement was achieved by a nano-structured gold surface. The proteins were immobilized via his-tags attached to the P side of the RC and the C-terminal end of the cytochrome (cyt) b subunit and co-reconstituted into a lipid bilayer by in-situ dialysis. In this configuration, the cyt c binding site of the two proteins is located on opposite sides of the membrane. Light-minus-dark absorbance spectra under continuous illumination in the absence of an electron donor indicated a slow quinone/semiquinone exchange, allowing release of ubiquinol (QH2) into the membrane. The interaction of the bc1 with QH2 was indicated by the stationary state obtained but only in the presence of cyt c. The interaction is discussed in terms of a semiquinone species formed in the course of the Q cycle mechanism of the bc1.
Keywords
Photosynthetic Reaction Centers, Bc1 Complexes, R. Sphaeroides, Surface-Enhanced Infrared-Absorption Spectroscopy, Nano-Structured Gold Surface, Ubiquinol, Semiquinone, His-Tags
To cite this article
Vedran Nedelkovski, Andreas Schwaighofer, Andreas F. Geiss, Christina Bliem, Renate L. C. Naumann, Interactions of Photosynthetic Reaction Centers with Bc1 Complexes from Rhodobacter Sphaeroides Studied Using SEIRAS on a Nano-Structured Gold Surface, International Journal of Bioorganic Chemistry. Vol. 2, No. 2, 2017, pp. 61-69. doi: 10.11648/j.ijbc.20170202.13
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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