Effects of Optimization Condition on Solid State Fermentation of Various Agro-Allied Waste for Production of Amylase Enzyme
International Journal of Bioorganic Chemistry
Volume 2, Issue 1, March 2017, Pages: 22-29
Received: Nov. 15, 2016; Accepted: Dec. 16, 2016; Published: Mar. 1, 2017
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Authors
Anaegbu Chinonso Joel, Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria
Orukotan Abimbola Ayodeji, Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria
Mohammed Sani Sambo Datsugwai, Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria
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Abstract
The effects of optimization condition on solid state fermentation of agro-allied waste for production of amylase using Bacillus sp was investigated. The amylase producing specie, was isolated from soil samples, rotten potatoe and spoiled fruit waste. Optimization of the amylase production using various agro-allied waste such as wheat bran, potatoe peel, Banana peel and rice bran were carried out. All the Bacillus sp isolates were screened by activity zone techniques with iodine solution. Out of 6 bacterial specie, only four (4) of the isolates showed positive results for amylase production. Bacterial strain-3 was found maximum at pH 6. The result showed that potatoe peel produced more enzyme activity under optimization conditions using Tap water, maltose, at pH 6, enzyme activity in the range of 0.555 iu/ml, 0.365 iu/ml, and 0.364 iu/ml respectively. Rice bran produced more enzyme activity of 0.342 iu/ml at 30°C while wheat bran showed an increased enzyme activity using peptone as source of nitrogen at 0.371 iu/ml, than any of the other agro-allied waste used for the amylase production. Owing to the prolific use of thermostable alpha amylase in various industries like paper, food, detergent, brewing and starch liquefaction process, the production of alpha amylase is still going on.
Keywords
Alpha Amylase, Optimization, Fermentation, Solid State Fermentation, Substrate Activity
To cite this article
Anaegbu Chinonso Joel, Orukotan Abimbola Ayodeji, Mohammed Sani Sambo Datsugwai, Effects of Optimization Condition on Solid State Fermentation of Various Agro-Allied Waste for Production of Amylase Enzyme, International Journal of Bioorganic Chemistry. Vol. 2, No. 1, 2017, pp. 22-29. doi: 10.11648/j.ijbc.20170201.14
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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