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Evaluation of Metabolites of Clarias gariepinus Exposed to Sub-Lethal Concentrations of Oilfield Wastewater
International Journal of Ecotoxicology and Ecobiology
Volume 2, Issue 4, December 2017, Pages: 150-157
Received: Mar. 20, 2017; Accepted: Oct. 31, 2017; Published: Nov. 25, 2017
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Nedie Patience Akani, Department of Microbiology, Rivers State University, Port Harcourt, Nigeria
Adaobi Patricia Ugbomeh, Department of Animal and Environmental Biology, Rivers State University, Port Harcourt, Nigeria
Ugwemorubong Ujagwung Gabriel, Department of Fisheries and Aquatic Environment, Rivers State University, Port Harcourt, Nigeria
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Twenty-eight adult Clarias gariepinus (mean weight 205 ± 12.89g SD; mean length; 31.13 ± 3.82cm SD) were exposed to various concentrations (0. 10, 20, 30, 40, 50 and 60%) in quadruplicates of an oilfield wastewater and were investigated for its responses on metabolites in tissue (plasma, gill, liver, kidney and muscle) samples after 28 days. The result showed that in all the tissues tested the values of total protein, creatinin and total bilirubin in all the control (0%) were higher (p≤0.05) than at treatment level. Metabolites in the plasma differed (P≤0.05) between concentrations except for creatinine and total bilirubin. Albumin in the control and treated fish were similar (p≥0.05) except in plasma and liver which recorded lower values than their control (p≤0.05). Total urea recorded its highest value at the highest concentration (60%) ranging between 4.25±0.09 in kidney and 12.0±1.47 mmol/l in muscle. Generally, the values did not follow a particular trend except for albumin values that decreased with an increased concentration of the toxicant. Significant changes observed in the study suggest stress induced by the oilfield wastewater on C. gariepinus hence the advocating for proper treatment of the wastewater before discharge into the environment.
Clarias gariepinus, Oilfield Wastewater, Metabolites, Tissues
To cite this article
Nedie Patience Akani, Adaobi Patricia Ugbomeh, Ugwemorubong Ujagwung Gabriel, Evaluation of Metabolites of Clarias gariepinus Exposed to Sub-Lethal Concentrations of Oilfield Wastewater, International Journal of Ecotoxicology and Ecobiology. Vol. 2, No. 4, 2017, pp. 150-157. doi: 10.11648/j.ijee.20170204.13
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S. K. Gupta, and P. C. Gupta,. General and applied Ichthyology (Fish and Fisheries). Chand and Company, Ram Nager, New Delhi, 2008, 739pp.
FAO. Pattern of production of pulses and fish in Nigeria relative to the world (1965-1984). FAO, Rome, Italy. 1986, 74 pp.
E. O. Aina. Keynote Address, Towards Environmentally Sustainable development in the petroleum industry. Proceedings of the International Seminar on Environmental. Impact Assessment and waste management in the Petroleum industry. Fairchild designs and prints, Ibadan, 1996, pp 1-6.
P. G. C. Odeigah, O. Nuruddeen, and O. O. Amund. Genotoxicity of oilfield wastewater in Nigeria. Hereditas, 126, 161–167, 1997.
J. Wills. A survey of offshore oilfield drilling wastes and disposal techniques to reduce the ecological impact of sea dumping, the effect of discharges of produced waters. Ekologicheaya Vahktan Sakhalina (Sakhalina Environmental Watch) 25th May, Sakhalina, London, 2000, pp. 1-5.
H. J. Somerville, D. Benneth, J. N. Davenport, M. S. Holt, A. Lynes, A. Mahieu, B. Mccourt, J. G. Parker, R. R. Stephenson, R. J. Watkinson and T. G. Wilkinson. Environmental Effect of Produced Water from North Sea Oil Operations. Marine Pollution Bulletin, 18(10), 549-558. 1987.
O. Obire, P. C. Adigwe, R. P. Ramesh and R. N. Okigbo. Effect of oilfield wastewater on microbiota of a freshwater in Nigeria. Journal of Pharmacy Research, 1(2), 264–270, 2008.
C. B. Koons, C. D. Mcauliffe and F. T. Weiss. Environmental aspects of produced waters from oil and gas extraction operations in offshore and coastal waters. Journal of Petroleum Technology, 29, 723–729, 1977.
O. O. James. Effect of Produced Water discharged from Imo River Flow Station SPDC (East) on Aquatic Environment. M. Sc. Thesis. Federal University of Technology, Owerri, 1999, pp 1–6.
V. E. Forbes, A. Palmqvist and L. Bach, The use and misuse of biomarkers in ecotoxicology. Environ. Toxicol. Chem., 25(1), 272-280, 2006.
V. Luskova, M. Svoboda and J. Kolarova. The effects of diazinon on blood plasma biochemistry in Carp (Cyprinus carpio L.). Acta Veterinaria Brno, 71, 117–123, 2002.
H. O. Nwamba, C. E. Akinanu and K. C. Oyekwelu. Effect of crude oil and its products on bilirubin of African Catfish (Clarias gariepinus). Animal Research International, 3(3), 531–533, 2006.
T. O. Sunmonu and O. B. Oloyede. Haematological Response of African Catfish (Clarias gariepinus) and Rat to Crude Oil Exposure. The Internet Journal of Hematology, 4, 1, 2008.
K. M. Adamu and O. Kori-Siakpere. Effects of sub-lethal concentrations of tobacco (Nicotiana tobaccum) leaf dust on some biochemical parameters of Hybrid catfish (Clarias gariepinus and Heterobranchus bidorsalis). Brazilian Archives of Biology and Technology, 54(1), 183-196, 2011.
S. M. Monteiro, J. M. Mancera, A. F. Fernandes and M. C. Sousa. Opper induced alterations of biochemical parameters of the gill and plasma of Oreochromis niloticus. Comparative Biochemistry and Physiology, 141, 375–383, 2005.
I. R. Iyang, and S. Thomas. Toxicity of Fluazifop-p-butyl on blood cells and metabolites of a common African Catfish (Clarias gariepinus). Nigerian Journal of Agriculture, Food and Environment. 12(2), 128–132, 2016.
N. P. Akani, and O. Obire. Mycological flora of Clarias gariepinus exposed to an oilfield wastewater in Nigeria. International Journal of Microbiology and Mycology. 3(1), 1–9. 2015.
N. P. Akani, and O. Obire. Bacterial Populations of Clarias gariepinus (Burchell 1822) exposed to an oilfield wastewater in Rivers State, Nigeria. Asian. Journal of Biological Sciences 7(5): 208-216, 2014.
N. P. Akani and U. U. Gabriel. Haematological responses of the African catfish Clarias gariepinus exposed to sublethal concentrations of an oilfield wastewater in Nigeria. Journal of Aquatic Sciences, 30(1B), 193–206, 2015.
N. P. Akani and E. R. Daka. Evaluation of weight changes, condition factor and Organosomatic indices of Clarias gariepinus exposed to sub-lethal concentrations of an Oilfield wastewater. Current Studies in Comparative Education, Science and Technology, 2(2), 338–355, 2015.
N. P. Akani and U. U. Gabriel. Enzymatic Responses of Clarias gariepinus (Burchell, 1822) exposed to sub-lethal concentrations of an oilfield wastewater. Journal of Applied Science and Biotechnology, 4(05), 026–032, 2016.
FAO. Food and Agricultural Organization. Meeting on the toxicity and bioaccumulation of selected substances in marine organisms. FAO Fisheries Report, No 334, Rovinj, Yugoslavia, 5–9 Nov. 1984 FIR/R334.
R. M. Gurure. Influence of two organo-chloride pesticides, thiodan and lindance on survival of fingerlings of O. niloticus and T. zilli. African Regional Aquaculture Centre Working Paper, 1978. ARAC/WP.6/87.
APHA. American Public Health Association. Standard Methods for the Examination of Water and Wastewater. 20th edition, American Public Health Association, American Water Works Association and Water Environment Federation, Washington D. C., U.S.A. 1998.
H. P. Sterling. Chemical and Biological Methods of Water Analysis for Aquaculturists. Pisces Press Limited Scotland, 1999.
C. T. I. Odu, O. F. Esuruoso, L. G. Nwaboshi and J. A. Ogunwale. Environmental Study of the Nigerian Agip Oil Company operational Area. Soil and Fresh Water Vegetation. Union Grafts Publics. Milan, 1985.
J. O’chei and A. Kolhatkar. Medical Laboratory Science: Theory and Practice. Tata Mcgraw Hill Publishing Co. Ltd. 2008, 1338pp.
A. C. Camus, R. M. Burrow, W. G. Hemstreet, R. L. Thure and J. P. Hawke. Aeromonas Bacterial infections – Motile Aeromonas septicemia. Southern Regional Aquaculture Centre Publication No.478, 1998.
R. Richards. Disease of Aquarium Fish The Veterinary Record, 101(9), 166–167, 1977.
S. A. Wemedo, O. Obire and N. P. Akani. Bacterial population of an Oilfield Wastewater in Nigeria. Asian Journal of Biological Sciences, 5(10), 46-51, 2012.
O. Obire and F. O. Amusan. The environmental Impact of Oilfield Formation Water on a Freshwater stream in Nigeria. Journal of Applied Science and Environmental Management, 7(1), 61–66, 2003.
FEPA. Guidelines and Standards for Environmental Pollution Control in Nigeria. Federal Environmental Protection Agency. Abuja. Nigeria. 1991, Pp.54–58.
R. B. Clerk. Marine Pollution. Clarandan Press, Oxford, 1986, 120-131.
M. Ozmen, A. Güngördü, F. Z. Kucukbay and R. E. Gülar. Monitoring the effect of water pollution in Cyprinus carpio in Karakara Lake, Turkey. Ecotoxicology, 15, 157-169, 2006.
B. L. Umminger. Relation of the whole blood sugar concentration in vertebrate to standard metabolic rate. Comparative Biochemistry and Physiology, 55, 457–460, 1977.
B. K. Das and S. C. Mukherjee. Toxicity of cypermethrin in Labeo rohita fingerlings, Biochemical enzymatic and haematological consequences. Comparative Biochemistry and Physiology c-Toxicology and Pharmacology, 134, 109-121, 2003.
S. Tiwari and A. Singh. Metabolic changes in the Snake head fish Channa punctatus, due to lattices of Euphorbia royleana, Asian Fisheries Science, 16, 147–155, 2003.
P. Reeta, S. Bhargava and D. K. Saraf. Toxic effects of some biocides on total serum protein in Heteropneustes fossilis. Indian Journal of Environmental Toxicologyy, 3, 5–6, 1993.
S. Ravichandran, K. Midhunashanthi and N. Indira. Impact of phenol on protein metabolism in the freshwater fish Oreochromis mossambicus. Journal of Ecotoxicology and Environmental Monitoring, 4, 33–37. 1994.
S. P. Bradbury, D. M. Symonic, J. R Coats and G. J. Atchison. Toxicology of fenvalerate and its constituent's isomers to the fathead minnow (Pimephales promelas) and blue gill minnow (Lepomis macrochirus). Bulletin of Environment, Contamination and Toxicology, 38, 727-735, 1987.
P. Y. Ram, S. Digvijay, S. K. Singh and A. Singh. Metabolic changes in fresh water fish Channa punctatus due to stem-bark extract of Crton tiglium. Pakistan Journal of Biological Sciences, 6, 1223–1228, 2003.
L. H. Jee, F. Marsroor and J. Ch. Kang. Response of cypermethrin- induced stress in haematological parameters of Korean rockfish, Sebastes schlegeli (Hilgendrof). Aquaculture Research, 36, 898-905, 2005.
O. O. Fafioye, S. O. Fagade and A. A. Adebisi. Toxicity of Raphia vinifera P. beauv fruit extracts on biochemical composition of Nile tilapia (Oreochromis niloticus, Trewavas). Biokemistri, 17 (2), 137-142, 2005.
R. K. Murray, D. K. Sranner, P. A. Mayes and V. W. Rodwell.. Biochemia Harpera (Harpers Biochemistry) 1995, PZWL, Warszawa.
M. T. Yakubu, L. S. Bilbis, M. Lawal and M. A. Akanji. Evaluation of selected parameters of rats' liver and kidney function following repeated administration of yohimbie. Biokemistri, 15 (2), 50–56, 2003.
K. Khleifat, J. Shakhanbeh and K. Tarawneh. The chronic effects of Teuerium pollum on some blood parameters and Histopathology of the liver and kidney in the rat. Turkish Journal of Biology, 26, 65–71, 2002.
U. M. Mahmoud, I. A. Mekkawy and A. A. Ibrahim. Biochemical response of the African catfish, Clarias gariepinus (Burchell, 1822) to sublethal concentrations of mercury chloride with supplementation of selenium and vitamin E. Toxicology and Environmental Health Sciences, 4(4), 218–234. 2013.
O. Kori-Siakpere. Acute toxicity of Potassium permanganate to fingerlings of the African catfish, Clarias gariepinus (Burchell1822). African Journal of Biotechnology, 7(14), 2514–2520, 2008.
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