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Electron Microscopy (SEM-EDAX & TEM) Studies on Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus)
International Journal of Ecotoxicology and Ecobiology
Volume 2, Issue 3, September 2017, Pages: 119-124
Received: Jun. 9, 2017; Accepted: Jun. 16, 2017; Published: Jul. 25, 2017
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Authors
Hosam Saleh, Fish Diseases Lab., National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Fatma A. S. Mohamed, Fish Diseases Lab., National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Mohamed Abd Al-Razik, Fish Breeding & Production Department, National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Nader Mahmoud, Fish Diseases Lab., National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Abeer H. Younis, Biotechnology Lab., National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Madiha Salah Ibrahim, Department of Microbiology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt
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Abstract
A field study on Streptococcus infection as a major cause for epidemic infections in Nile Tilapia aquaculture in Kafr El-Shikh Governorate was conducted. This study was carried out to study the gross lesions and post mortem finding of Streptococcsis in Nile Tilapia. Streptococcus was isolated, then identified by Electron Microscopy (Scanning Electron Microscope (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscope (TEM)) to investigate the phenotypic characteristics, ultra-structure of the bacterial cells. Clinical signs of the infected fish showed presence of hemorrhagic patches on different external body regions, ascitis and exophthalmia. Post mortem examinations revealed presence of inflammation of the liver, spleen and kidney. Microbial identification by Gram stain showed Gram positive cocci in shape arranged in chains and the biochemical tests displayed negative catalase and oxidase tests. Electron Microscopy (SEM, EDAX & TEM) confirmed these results and showed the clear cocci shape of streptococcus species and the arrangement of the chains with a bacterial average length of 700 nm. The EDAX results revealed that the elemental structure of the bacterial sample were C,N,O with the concentration 46.93%, 16.61% and 36.46%, respectively.
Keywords
Streptococcus species, Oreochromis niloticus, SEM, TEM, EDAX
To cite this article
Hosam Saleh, Fatma A. S. Mohamed, Mohamed Abd Al-Razik, Nader Mahmoud, Abeer H. Younis, Madiha Salah Ibrahim, Electron Microscopy (SEM-EDAX & TEM) Studies on Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus), International Journal of Ecotoxicology and Ecobiology. Vol. 2, No. 3, 2017, pp. 119-124. doi: 10.11648/j.ijee.20170203.14
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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