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Interactive Effect of Trichoderma viride on Broad Bean (cv. Vicia faba L.) Genotypes Grown Under Different Salinity Stress Conditions
International Journal of Ecotoxicology and Ecobiology
Volume 1, Issue 3, December 2016, Pages: 11-141
Received: Nov. 23, 2016; Accepted: Dec. 7, 2016; Published: Jan. 5, 2017
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Abdel Kareem S. H. Mohamed, Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt
Mahmoud G. Mahmoud, Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt
Abd El-Monem M. Sharaf, Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cario, Egypt
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An investigation was undertaken to evaluate the interaction effect of Trichoderma viride for their possible role in imparting stress resistance and provide insight in to the potential of broad bean (cv. Vicia faba L.) genotypes to adapt to saline conditions. For this, broad bean genotypes (Assiut1, Assiut16 and Assiut159) were treated with different salinity stress levels (00, 75, 150 and 250 mM NaCl) singly or in combination with Trichoderma in the presence of salinity. In the obtained results, the overall plant growth parameters such as shoot fresh, dry weight and physiological, bio-chemical activities and antioxidant enzyme activities (catalase and peroxidase) were measured after 27 days of plant harvest. The interaction results showed that the effect of salinity stress was significantly reduced due to application of Trichoderma in terms of plant growth or in the case of Na+ accumulation in plant cells. In defense related physiological, biochemical and antioxidant enzyme activity also showed marked increase due to single or in combination of Trichoderma with salinity. Moreover, the interactive effects of Trichoderma were more pronounced in increasing overall growth, reducing transport of Na+ from root to shoot to save cytoplasm from the toxic effect of salinity and bringing about defense related physiological, biochemical, antioxidant enzyme activities in the tested-broad bean genotypes.
Broad Bean, Trichoderma viride, Antioxidant Enzymes, H2O2, MAD, Salinity Stress
To cite this article
Abdel Kareem S. H. Mohamed, Mahmoud G. Mahmoud, Abd El-Monem M. Sharaf, Interactive Effect of Trichoderma viride on Broad Bean (cv. Vicia faba L.) Genotypes Grown Under Different Salinity Stress Conditions, International Journal of Ecotoxicology and Ecobiology. Vol. 1, No. 3, 2016, pp. 11-141. doi: 10.11648/j.ijee.20160103.21
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
M. Ashraf and M. R. Foolad “Improving plant abiotic-stress resistance by exogenous application of osmoprotectants glycinebetaine and prolin”, Env. Exp. Bot., 2007, 59: 206-216.
A. S. H Mohamed and M. A. K. Shaddad “Effect of salinity on dry matter production, ion accumulation, some metabolites and antioxidant enzymes in wheat and broad bean”, Al Azhar Bull., 2013, 24, No. 2: 149-164.
R. Fortmeier and S. Schubert “Salt tolerance of maize (Zea mays L.): The role of sodium exclusion”, Plant Cell Environ., 1995, 18: 1041–1047.
C. Zörb, A. Noll, S. Karl, K. Leib, F. Yan, and S. Schubert “Molecular characterization of Na+/H+ antiporters (ZmNHX) of maize (Zea mays L.) and their expression under salt stress”, J. Plant Physiol., 2005, 162: 55–66.
B. Pitann, A. Mohamed, A. Neubert and S. Schubert “Tonoplast Na+/H+ antiporters of newly developed maize (Zea mays) hybrids contribute to salt resistance during the second phase of salt stress”, J. Plant Nutr. Soil Sci., 2013, 176 (2): 148-156.
R. Munns “Genes and salt tolerance: bringing them together”, New Phytol., 2005; 167: 645–663.
H. X. Zhang and E. Blumwald “Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit”, Nat. Biotech., 2001; 19, 765-768.
R. K. Sairam., K. V. Rao and G. C. Srivastava “Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration”, Plant Sci., 2002, 163, 1037–1046.
A. Sabra, F. Daayf and S. Renault “Differential physiological and biochemical responses of three Echinacea species to salinity stress”, Scientia Hort., 2012, 135 (24): 23-31.
F. Eyidogan and M. T. Öz “Effect of salinity on antioxidant responses of chickpea seedlings”, Acta Physiol Plant., 2007, 29:485-493.
K. Apel and H. Hirt “Reactive oxygen species: metabolism, oxidative stress, and: metabolism, oxidative stress, and signal transduction”, Annu. Rev. Plant Biol., 2004, 55: 373–399.
S. Masood, L. Saleh, K. Witzel C. Plieth and K. H. Mühling “Determination of oxidative stress in wheat leaves as in fluenced by boron toxicity and NaCl stress”, Plant Physiol. Biochem., 2012, 56: 56 - 61.
A. Parida, A. B. Das and P. Das “NaCl stress causes changes in photosynthetic pigments, proteins, and other metabolic components in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures”, J. Plant Biol., 2002, 45: 28–36.
A. Hashem, E. F Abd-Allah, A. A. Alqarawi, A. A. Al Huqail and D. Egamberdieva “Alleviation of abiotic salt stress in Ochradenus baccatus (Del.) by Trichoderma hamatum (Bonord.) Bainier”, J. Plant Interact., 2014, 9: (1) 857-868.
G. E. Harman, C. R. Howell, A. Viterbo, I. Chet, and M. Lorito “Trichoderma species-Opportunistic, avirulent plant symbionts ”, Nat. Rev. Microbiol., 2004, 1, 43-56.
A. Fini, L. Guidi, F. Ferrini, C. Brunetti, M. Di Ferdinando, S. Biricolti, S. Pollastri, L. Calamai, and M. Tattini “Drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in Fraxinus ornusleaves: an excess light stress affair?”, J. Plant Physiol., 2012, 169: 929–939.
F. Eyidogen, and M. T. Oz “Effect of salinity on antioxidant responses on chickpea seedlings”, Acta. Physiol. Plant., 2007, 29, 485-493.
U. Deinlein, A. B. Stephan, T. Horie, W. Luo, G. Xu and J. I. Schroeder “Plant salt-tolerance mechanisms”. Trends in Plant Sci., 2014, 19, 371-379.
M. L. Dionisio-sese and S. Tobita “Effects of salinity on sodium content and photosynthetic responses of rice seedlings differing in salt tolerance”, J. Plant Physiol., 2000, 157, 54-58.
M. Shoresh, G. E. Harman and F. Mastouri “Induced systemic resistance and plant responses to fungal biocontrol agents”, Annu. Rev. Phytopathol., 2010, 48: 21–43.
V. Balbi and A. Devoto “Jasmonate signaling network in Arabidopsis thaliana: crucial regulatory nodes and new physiological scenarios”, New Phytol., 2008, 177, 301–318.
R. Hermosa, A. Viterbo, I. Chet and E. Monte “Plant-beneficial effects of Trichoderma and of its genes”, Microbiol., 2012, 158, 17–25.
K. D. Rawat, M. Chahar, P. V. Reddy, P. Gupta, N. Shrivastava, U. D. Gupta, M. Natrajan, V. M. Katoch, K. Katoch, and D. S. Chauhan “Expres- sion of CXCL10 (IP-10) and CXCL11 (I-TAC) chemokines during Mycobacterium tuberculosis infection and immune prophylaxis with Mycobacterium indicus pranii (Mw) in guinea pig”, Infect Genet Evol., 2013, 13, 11-17.
I. Gal-Hemed, L. Atanasova, Z. M. Komon, I. S. Druzhinina, A. Viterbo, and O. Yarden “Marine Isolates of Trichoderma spp. as Potential Halotolerant Agents of Biological Control for Arid-Zone Agriculture”, Appl Environ Microbiol., 2011, 77: (15), 5100–5109.
H. O. Egberongbe, A. K. Akintokun, O. O. Babalola, and M. O. Bankole “The effect of Glomus mosseae and Trichoderma harzianum on proximate analysis of soybean (Glycine max (L.) Merrill.) Seed grown in sterilized and unsterilized soil”, J. Agri. Ext. Rural Develp., 2010, 2: (4), 54-58.
H. Evelin, R. Kapoor and B. Giri “Arbuscular mycorrhizal fungi in alleviation of salt stress”, a review, Annals of Bot., 2009, 104: 1263–1280.
G. N. Al-Karaki “Growth of mycorrhizal tomato and mineral acquisition under salt stress”, Mycorrhiza., 2000, 10: 51–54.
P. R John, R. D Tyagi, D. Prévost, B. Satinder, P. K. Stéphan, and R. Y. Surampalli “Mycoparasitic Trichoderma viride as a biocontrol agent against Fusarium oxysporum f. sp. adzuki and Pythium arrhenomanes and as a growth promoter of soybean Crop Protection”, 2010, 29: 1452-1459.
A. N. Shahzad “The Role of Jasmonic Acid (JA) and Abscisic Acid (ABA) in Salt Resistance of Maize (Zea mays L.)”, 2011,; VVB Laufersweiler Verlag Staufenbergring 15 D-35396 GIESSEN; ISBN: 978-3-8359-5829-6.
A. Sümer, C. Zörb, F. Yan, and S. Schubert “Evidence of sodium toxicity for the vegetative growth of maize (Zea mays L.) during the first phase of salt stress”, J. Appl. Bot., 2004, 78, 135–139.
H. Aebi “Catalase in Vitro. Methods in Enzymology” 1984, 105: 121–126.
S. P. Mukherjee and M. A. Choudhuri “Implications of water stress-induced changes in the level of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings”, Physiol. Plant., 1983, 58: 166–170.
R. K. V. Madhava and T. V. S. Sresty “Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses”, Plant Sci. 2000, 157: 113-128.
R. Munns “Physiological process limiting plant growth in saline soil: some dogmas and hypothesis”, Plant Cell Enviro., 1993, 16: 15-24.
J. B. Passioura “Water transport in and to roots”. Annu. Rev. Plant. Physiol. Plant. Mol. Biol., 1988, 39:245- 265.
Q Gong, P. Li, S. Ma, S. I. Rupassara and H. Bohnert “Salinity stress adaptation competence in the extremophile T. halophila in comparison with its relative A. thaliana”. Plant J. 2005, 31: 826-839.
I. Schwob, M. Ducher, H. Sallanon, and A. Coudret “Trees Strucr. Funcr”, 1998, 12: 236-240.
M. S. A. Saleh Increased heavy metal tolerance of cowpea plants by dual inoculation of an arbuscular mycorrhizal fungi and nitrogen-fixer Rhizobium bacterium”, Afr. J. Biotechnol. 2006, 5: 132–144.
A. A. Radi, F. A Faraghaly and M. A. Hamada “Physiological and biochemical responses of salt-tolerant and salt-sensitive wheat and bean cultivars to salinity”, 2012, 78: 135-139.
M. Tester and R. Davenport “Na+ tolerance and Na+ transport in higher plant”, Annal. Bot., 2003, 91: 523-527.
S. Eker, G. Cömertpay, Ö. Konufikan, A. ÜLGER, L Öztürk, and I. Çakmak “Effect of salinity stress on dry matter production and ion accumulation in hybrid maize varieties”, Turkish J. of Agricult. Forest. 2006, 30: 365-373.
G. J. Alberico, and G. R. Cramer “Is the salt tolerance of maize related to sodium exclusion? I. Preliminary screening of seven cultivars”, J. Plant Nutr. 1993, 16: 2289-2303.
L. Erdei and E. Taleisnik “Changes in water relation parameters under osmotic and salt stresses in maize and sorghum”, Physiol. Plant. 1993, 89: 381-387.
A. D. Neto and J. N. Tabosa “Salt stress in maize seedlings: I. Growth analysis”, Revista Brasileira de Engenharia agrícolae Ambiental., 2004, 159-164.
A. R. Yeo and T. J. Flowers “Accumulation and localization of sodium ions within the shoot of rice (Oryza sativa) varieties differing in salinity resistance”, Physiol. Plant., 1982, 56: 343–348.
A. R. Yeo, S. J. M. Capom and T. J. Flowers “The effect of salinity upon photosynthesis of rice (Oryza sativa L.): gas exchange by individual leaves in relation to their salt content”, J. exp. Bot. 1985, 36: 1240-1248.
Z. Aslam, M. Salim, R. H. Quresh, and G. R. Sandhu “Salt tolerance of Echinochloa crusgalli”, Biol. Plant., 1987, 29: 66-69.
H. Wang, Z. Wu, Y. Zhou, J. Han and D. Shi “Effects of salt stress on ion balance and nitrogen metabolism in rice”, Plant Soil Environ., 2012, 58: 62-67.
F. J. M. Maathuis and A. Amtmann “K+ nutrition and Na+ toxicity: The basis of cellular K+/Na+ ratios”, Annal. Bot., 1999, 84: (2), 123-133.
A. Wakeel, S. Hanstein, B. Pitann and S. Schubert “Hydrolytic and pumping activity of H+- TPase from leaves of sugar beet (Beta vulgaris L.) as affected by salt stress”, J. of Plant Physiol., 2010, 167: 725-731.
J. Q. Song, X. R. Mei, and H. Fujiyama “Adequate internal water status of NaCl-salinized rice shoots enhanced selective calcium and potassium absorption”, Soil Sci. Plant Nutr., 2006, 52: 300-304.
E. Blumwald, S. A. Gilad, and P. M. Apse “Sodium transport in plant cells”, Biochimica et Biophysica Acta., 2000, 140-151.
C. Zörb, A. Noll, S. Karl, K. Leib, F. Yan, S. Schubert “Molecular characterization of Na +/H+ antiporters (ZmNHX) of maize (Zea mays L.) and their expression under salt stress”, J. Plant Physiol., 2005, 162: 55–66.
A. Wakeel, M. Gul, and M. Sanaullah “Potassium Dynamics in three alluvial soils differing in Clay Contents”, Emir. J. Food Agric., 2013, 25: 39-44.
M. P. Apse, G. S. Aharon, W. A. Snedden and E. Blumwald “Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis”, Science, 1999, 285: 1256–1258.
M. P. Apse, J. B. Sottosanto and E. Blumwald “Vacuolar cation/H+ exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of AtNHX1, the Arabidopsis vacuolar Na+/H+ antiporter”, J. Plant., 2003, 36: 229–239.
M. L. Dionisio-sese, and S. Tobita “Effects of salinity on sodium content and photosynthetic responses of rice seedlings differing in salt tolerance”, J. Plant. Physiol., 2000, 157: 54-58.
F. Mastouri, T. Bjorkman and G. E. Harman “Trichoderma harzianum enhances antioxidant defense of tomato seedlings and resistance to water deficit”, Mol. Plant Microbe Interact., 2012, 25: 1264–1271.
R. G. Alscher, N. Erturk and L. S. Heath “Role of superoxide dismutases (SODs) in controlling oxidative stress in plants”, J. Exp. Bot., 2002, 53: 1331–1341.
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