International Journal of Ecotoxicology and Ecobiology
Volume 1, Issue 3, December 2016, Pages: 103-110
Received: Oct. 31, 2016;
Accepted: Nov. 17, 2016;
Published: Dec. 17, 2016
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Samy A. Saber, Departmentof Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt
Boshra A. ElSalkh, Departmentof Zoology, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt
Aml S. Said, Departmentof Zoology, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt
Rashad E. M. Said, Departmentof Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt
Ali G. Gadel-Rab, Departmentof Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt
Many amphibians occupy appositional roles between different animal taxa; despite this, many species are threatened with decline and extinction. Several and different contributors and factors were previously reviewed to contribute amphibian disappearance. Between those factors are chemical pollution, heavy metals, climate change, disease, etc. The most commonly used index of developmental instability is fluctuating asymmetry (FA), as a feature of environmental impact was also reviewed. F.A is the variance in right minus left values of bilaterally symmetrical structures. The results indicate that individuals exposed to atrazine or that exposed to both atrazine and nitrate exhibit a significantly higher degree of FA (p<0.01) while FA measured in individuals treated only with nitrate was not significant (p<0.05) These results constitute the first assessment of FA in this species in Egypt and suggest that the degree of FA in skeletal traits cane be a useful indicator of the degree of environmental stress experienced by amphibian populations.
Samy A. Saber,
Boshra A. ElSalkh,
Aml S. Said,
Rashad E. M. Said,
Ali G. Gadel-Rab,
Limbs Asymmetry as Biomarker in the Egyptian Toad Amietophrynus regularis Exposed to Atrazine and Nitrates, International Journal of Ecotoxicology and Ecobiology.
Vol. 1, No. 3,
2016, pp. 103-110.
Z. M. Zhelev, A. D, Arnaudov, G. S. Popgeorgiev, and H. A. Dimitrov, “Assessment of ecological status of two rivers with different types of anthropogenic pollution in Southern Bulgaria based on the level of fluctuating asymmetry in the populations of marsh frog Rana ridibunda (Amphybia: Ranidae),” Acta. Zool. Bulg., 4, 2012: 225-231.
Z. M. Zhelev, G. S. Popgeorgiev, Z. K. Georgieva, “Fluctuating Asymmetry in the Populations of Pelophylax ridibundus and Pseudepidalea viridis (Amphibia: Anura) in the Region of the Lead and Zinc Plant “Kardzhali” (South Bulgaria),” Acta. Zool. Bulg., 2014, 66 (1): 83-87.
Z. M. Zhelev, Georgi S. Popgeorgiev, Atanas D. Arnaudov, Katerina N. Georgieva and Nikolay H. Mehterov, “Fluctuating asymmetry in Pelophylax ridibundus (Amphibia: Ranidae) as a response to anthropogenic pollution in south Bulgaria,” Arch. Biol. Sci., Belgrade,2015, 67(3): 1009-1023.
R. G. Wetzel, “Limnology: Lake and River Ecosystems” 3rd ed. Academic, New York, NY, USA., 2001.
G. R. Smith, C. J. Dibble, A. J Terlecky, C. B. Dayer; A. B. Burner, and M. E Ogle, “Effects of mosquito fish and ammonium nitrate on activity of green frog (Lithobates clamitans) tadpoles,”: a mesocosm experiment, J. Freshwater Ecol., 2011, 26: 1, 59-63.
W. H. Schlesinger, “Biogeochemistry: An Analysis of Global Change” 2nd ed. Academic, Burlington, MA, USA. Schlesinger, 1997.
APHA “Standard methods for the examination of water and wastewater,” 17th ed. Am. Publi. Health. Ass., 1989, Washington, DC.
A. G. M. Osman, A. M. Abd El Reheem, A. M Moustafa, W. Kloas, U. M. Mahmoud, and K. Y. Abuel-Fadl, “In situ evaluation of the genotoxic potential of the river Nile: I. Micronucleus and nuclear lesion tests of erythrocytes of Oreochromis niloticus niloticus” (Linnaeus, 1758) and Clarias gariepinus (Burchell, 1822), Toxicol.& Environ.Chem., 2011, 93(5): 1002-1017.
E. Sherman, K. Tock, and C. Clarke, “Fluctuating asymmetry in Ichthyophonus-sp. infected newts, Notophthalmus viridescens, from Vermont,” Appl. Herpetol., 2009, 6(4): 369–378.
F. Sِöderman, S. Van Dongen, S. Pakkasmaa, and J. Merilä, “Environmental stress increases skeletal fluctuating asymmetry in the moor frog Rana arvalis,” Oecologia, 2007, 1(51):593–604.
R. E. M. Said, " Using of some amphibians as bioindicator of aquatic pollution of Egyptian river Nile" PhD thesis, Department of Zoology–Faculty of Science, Al-Azhar University, Assiut, Egypt.
A. R. Alford, and J. S. Richards, Global amphibian declines: a problem in applied ecology. Annu. Rev. Ecol. Evol. Syst., 1999, 300:133–165.
A. R. Blaustein, and J. M. Kiesecker, “Complexity in conservation: Lessons from the global decline of amphibian populations,” Ecol Lett., 2002, 5:597–608.
K. R. Solomon, J. A. Carr, L. H. Du Preez, J. P. Giesy, R. J. Kendall, E. E. Smith, G. J. Van Der Kraak, “Effects of atrazine on fish, amphibians, and aquatic reptiles: a critical review,” Crit. Rev. Toxicol., 2008,38(9):721-72.
K. R. Solomon, D. B. Baker, R. P. Richards, D. R. Dixon, S. J. Klaine, T. W. LaPoint, R. J. Kendall, C. P. Weisskopf, J. M. Giddings, J. P. Giesy, L. W. Hall, and W. M. Williams, “Ecological risk assessment of atrazine in North American surface waters,” Environ. Toxicol. Chem., 1996, 15:31–74.
T. B. Hayes, V. Khoury, A. Narayan, M. Nazir, A. Park, T. Brown, L. Adame, E. Chan, D. Buchholz, T. Stueve, and S. Gallipeau, “Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis). Proc. Natl. Acad. Sci. U S A., 2010, 107(10): 4612-4617.
M. Marco, C. Quilchano, and A. R. Blaustein, “Sensitivity to nitrate and nitrite in pond–breeding amphibians from the Pacific Northwest,” USA. Environ. Toxicol. Chem., 1999, 18:2836–2839.
J. D. Rouse, C. A. Bishop, J. Struger, “Nitrogen pollution. An assessment of its threat to amphibian survival. Environ. Health Perspect., 1999, 107(10):799–803.
A. A. Burgett, C. D. Wright, G. R. Smith, D. T. Fortune, S. J. Johnson, “Impact of ammonium nitrate on Wood Frog (Rana sylvatic) tadpoles: Effects on survivorship and behavior,” Herpetol. Conserv. Biol., 2007, 2(1):29-34.
J. N. Galloway, F. J. Dentener, D. G. Capone, E. W. Boyer, R. W. Howarth, S. P. Seitzinger, G. P. Asner, C. Cleveland, P. Green, E. Holland, Karl, D. M, A. F. Michaels, J. H. Porter, A. Townsend, C. Vo¨ro¨smarty, “Nitrogen cycles: Past, present, and future,” Biogeochemistry, 2004., 70:153–226.
S. G. Diana, and V. R. Beasley, “Amphibian toxicology” In Lannoo M. J, ed, Status and Conservation of Midwestern Amphibians. University of Iowa, Iowa City, IA, USA, 1998, pp 266–277.
G. S. Schuytema, and A. V. Nebeker, “Comparative toxicity of ammonium and nitrate compounds to Pacific treefrogand African clawed frog tadpoles” Environ. Toxicol.Chem., 1999. 18: 2251–2257.
K. L. Gosner, “A simplifed table for staging anuran embryos and larvae with notes on identication. Herpetol” 1960, 16:183–190.
M. Tandy, M. Rödel, M. Largen, J. Poynton, S. Lötters, S. Baha El Din, and H. Gerson,"Amietophrynus regularis". IUCN Red List of Threatened Species. Version 2013.2. International Union for Conservation of Nature (IUCN)., 2004, Retrieved 2014-09-26.
L. Ezemonye, and I. Tongo, “Acute toxic effects of Endosulfan and Diazinon pesticides on adult amphibians ( Bufo regularis),” J. Environ. Chem. Ecotoxicol., 2010, 2(5):73-78.
Robins, A. and L. J. Rogers, “Limb preference and skeletal asymmetry in the cane toad, Bufo marinus (Anura: Bufonidae),” Laterality, 2002, 7(3):261–275.
A. R. Palmer, and C. Strobeck, “Fluctuating asymmetry: measurement, analysis, patterns,” Ann Rev.Ecol.Syst., 1986, 17(1):391-421.
M. E. Ortiz-Santaliestra, A. Marco, M. J. Fernández, and M. Lizana, Inﬂuence of developmental stage on sensitivity to ammonium nitrate of aquatic stages of amphibians,” Environ. Toxicol. Chem., 2006, 25:105–111.
M. L. Harris, L. Chora, C. A. Bishop, and J. P. Bogart, “Species- and age-related differences in susceptibility to pesticide exposure for two amphibians, Rana pipiens, and Bufo americanus,” Bull. Environ. Contam. Toxicol., 2000, 64:263–270.
G. R. Smith, “Effects of acute exposure to a commercial formulation of glyphosate on the tadpoles of two species of anurans,” 2001, Bull. Environ. Contam. Toxicol., 67:483-488.
S. J. Hecnar, “Acute and chronic toxicity of ammonium nitrate fertilizer to amphibians from southern Ontario,” Environ. Toxicol. Chem., 14:2131–2137.
P. J. Watt, and R. S. Oldham, “The effect of ammonium nitrate on the feeding and development of larvae of the Smooth Newt, Triturus vulgaris (L.), and on the behaviour of its food source, Daphnia,” Freshwater Biol., 1995, 33:319-324.
B. R. Anholt, S. Negovetic, C. Ranta, and C. Som, “Predator complement determines the relative success of tadpoles of the Rana esculenta complex,” Evol.Ecol.Res., 2005, 7:733-741.
G. H. Dayton, and L. A. Fitzgerald, “Competition, predation, and the distributions of four desert anurans,” 2001, Oecologia, 129:430-435.
R. D. Semlitsch, “Asymmetric competition in mixed populations of tadpoles of the hybridogenetic Rana esculenta complex,” Evolution, 1993, 47:510-519.
J. Baker, and V. Waights, “The effect of sodium nitrate on the growth and survival of toad tadpoles (Bufo bufo) in the laboratory,” Herpetol. J., 1993, 3: 147–148.
J. Baker, and V. Waights, “The effects of nitrate on tadpoles of the tree frog (Litoria caerulea).,” Herpetol. J., 1994, 4: 106–108.
D. W. Huey, and T. L. Beitinger, “Hematological responses of larval Rana catesbiana to sublethal nitrite exposures,” Bull. Environ. Contam. Toxicol., 1980, 25: 574–577.
G. E. Howe, R. Gillis, and R. C. Mowbray, “Effect of chemical synergy and larval stage on the toxicity of atrazine and alachlor to amphibian larvae,” Environ. Toxicol. Chem., 1998, 17: 519–525.
J. W. Allran, and W. H. Karasov, “Effects of atrazine and nitrate on northern leopard frog (Rana pipiens) exposed in the laboratory from posthatch through metamorphosis,” Environ. Toxicol. Chem., 2000, 19:2 850 –2855.
G. Van Der Kraak, J. Matsumoto, M. Kim, and A. J. Hosmer” Atrazine and its degradates have little effect on the corticosteroid stress response in the zebrafish,” Comp. Biochem. Physiol., 2015, c, 170:1-7.
M. Levin, “Left -right asymmetry in embryonic development: a comprehensive review,” Mech. Dev., 2005, 122: 3- 25.
B. A. Pierce, “Acid tolerance in amphibians," Bioscience, 1985, 35, 239-243.
K. Räsänen, A. Laurila, and J. Merilä, “Geographic variation in acid stress tolerance of the moor frog, Rana arvalis," I. Local adaptation. Evolution, 2003, 57: 352-362.
K. Räsänen, A. Laurila, and J. Merilä, “Geographic variation in acid stress tolerance of the moor frog, Rana arvalis," II. Adaptive maternal effects. Evolution, 2003, 57, 363-371.
K. Räsänen, A. Laurila, and J. Merilä, “Maternal investment in egg size: environment- and population-specific effects on off springperformance,” Oecologia, 2005,142: 546-553.
C. H. Jagoe, and T. A. Haines Fluctuating asymmetry in fishes inhabiting acidified and unacidified lakes. J. Zool., 1985, 63, 130-138.
S. N. Stuart, J. S. Chanson, N. A. Cox, B. E. Young, A. S. L. Rodrigues, D. L. Fischman, and R.W. Waller, “Status and trends of amphibian declines extinctions worldwide Science 2004, 306:1783-1786.