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Occupational Toxicological Characteristics of LLM-105 Explosives
International Journal of Ecotoxicology and Ecobiology
Volume 1, Issue 3, December 2016, Pages: 88-93
Received: Sep. 7, 2016; Accepted: Oct. 12, 2016; Published: Oct. 28, 2016
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Yu Weifei, Laboratory of Energetic Materials, Institute of Chemical Materials, China Academy of Engineering Physics, Mianyang, China
Liao Longyu, Laboratory of Energetic Materials, Institute of Chemical Materials, China Academy of Engineering Physics, Mianyang, China
Chen Feilan, Center of Experimental Animals, Chongqing Medical University, Chongqing, China
He Mingzhong, Center of Experimental Animals, Chongqing Medical University, Chongqing, China
Tan Dongmei, Center of Experimental Animals, Chongqing Medical University, Chongqing, China
Fan Guijuan, Laboratory of Energetic Materials, Institute of Chemical Materials, China Academy of Engineering Physics, Mianyang, China
Hao Shilong, Laboratory of Energetic Materials, Institute of Chemical Materials, China Academy of Engineering Physics, Mianyang, China
Lu Huanchang, Laboratory of Energetic Materials, Institute of Chemical Materials, China Academy of Engineering Physics, Mianyang, China
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LLM-105, a novel nitro-substituted explosive, was evaluated for its occupational toxicological characteristics. Acute oral toxicity tests showed that the maximum tolerated dose should be 8% concentration (gastric irrigation 20mL/kg) and the minimum lethal dose should be 10% (gastric irrigation 20mL/kg). Acute dermal toxicity tests showed that LD50 should be more than 2000 mg/kg. Acute eye irritation tests showed that only slight irritations were found. Skin sensitization tests showed that slight allergic reactions were found. It was suggested that LLM-105 should be generally slightly toxic under normal process which should be positive as preliminary evaluation for occupational protection.
2, 6-diamino-3, 5-dinitropyrazine-l-oxide (LLM-105), Toxicological Evaluations, Occupational Toxicological Characteristics
To cite this article
Yu Weifei, Liao Longyu, Chen Feilan, He Mingzhong, Tan Dongmei, Fan Guijuan, Hao Shilong, Lu Huanchang, Occupational Toxicological Characteristics of LLM-105 Explosives, International Journal of Ecotoxicology and Ecobiology. Vol. 1, No. 3, 2016, pp. 88-93. doi: 10.11648/j.ijee.20160103.15
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This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Guilherme R. Lotufo, William Blackburn, Sydney J. Marlborough, John W. Fleeger, Toxicity and bioaccumulation of TNT in marine fish in sediment exposures, Ecotoxicology and Environmental Safety, 73, 1720–1727 (2010).
Jenkins T. F., Hewitt A. D., Grant C. L., Thiboutot S., Ampleman G., Walsh, M. E., Identity and distribution of residues of energetic compounds at army live-fire training ranges. Chemosphere, 63, 1280-1290 (2006).
Neuwoehner J., Schofer A., Erlenkaemper B., Steinbach K., Hund-Rinke K., Eisentraeger A., Toxicological characterization of 2,4,6-trinitrotoluene, its transformation products, and two nitramine explosives. Environmental Toxicology and Chemistry, 26, 1090-1099 (2007).
Hawari J., Shen C. F., Guiot S. R., Greer C. W., Rho D., Sunahara G., Bioremediation of highly energetic compounds: a search for remediation technologies. Water Sci. Technol. 42, 385-393 (2000).
Ryu H., Han J. K., Jung J. W., Bae B., Nam K., Human health risk assessment of explosives and heavy metals at a military gunnery range. Environ. Geochem. Health, 29, 259-269 (2007).
B. R. Flokstra, B. V. Aken, J. L. Schnoor, Microtox toxicity test: Detoxification of TNT and RDX contaminated solutions by poplar tissue cultures. Chemosphere, 71, 1970-1976 (2008).
George A Parker, Gunda Reddy, Michael A Major, Re-evaluation of a twenty-four-month chronic toxicity / carcinogenicity study of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in the B6C3F1 hybrid mouse. International Journal of Toxicology, 25(5), 373-378(2006).
Lynch J. C., Brannon J. M., Delfino J. J., Dissolution rates of three high explosive compounds: TNT, RDX, and HMX. Chemosphere, 47, 725–734 (2002).
Brunjes K. J., Severt S. A., Liu J., Pan X. P., Brausch J., Cox S. A., Effects of HMX exposure on reproduction and hatchling development in Northern bobwhite quail. J. Toxicol. Environ. Health A 70, 682-687 (2007).
Rosen G., Lotufo G. R., Toxicity of explosive compounds to the marine mussel, Mytilus galloprovincialis, in aqueous exposures. Ecotoxicol. Environ. Saf. 68, 228–236 (2007).
Smith J. N., Liu J., Espino M. A., Cobb G. P., Age dependent acute oral toxicity of hexahydro-1,3,5- trinitro-1,3,5-triazine (RDX) and two anaerobic N-nitroso metabolites in deer mice (Peromyscus maniculatus). Chemosphere 67, 2267–2273 (2007).
Kristin Elgh Dalgren, Sylvia Waara, Anders Duker, Thomas von Kronhelm, Patrick A. W. van Hees, Anaerobic bioremediation of a soil with mixed contaminants: explosives degradation and influence on heavy metal distribution, monitored as changes in concentration and toxicity. Water Air Soil Pollut. 202:301–313, (2009).
Jordan N. Smith, Jun Liu, Marina A. Espino, George P. Cobb, Age dependent acute oral toxicity of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and two anaerobic N-nitroso metabolites in deer mice (Peromyscus maniculatus). Chemosphere. 67, 2267–2273 (2007).
Kuperman R. G., Checkai R. T., Simini M., Phillips C. T., Kolakowski J. E., Kurnas C. W., Toxicities of dinitrotoluenes and trinitrobenzene freshly amended or weathered and aged in a sandy loam soil to Enchytraeus crypticus. Environmental Toxicology and Chemistry, 25, 1368-1375 (2006).
Sharon A. Meyer, Adam J. Marchand, Jennifer L. Hight, George H. Roberts, Lynn B. Escalon, Laura S. Inouye and Denise K. MacMillan, Up-and-down procedure (UDP) determinations of acute oral toxicity of nitroso degradation products of RDX. Journal of applied toxicity, 25: 427 - 434 (2005).
Meyer-SA Marchand-AJ Hight-JL Macmillan-DK, Acute Oral Toxicity of Nitroso Degradation-Products of Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (RDX), Toxicological sciences, 72(1), pp 780-780 (2003).
Fournier D., Halasz A., Spain J., Fiurasek P., Hawari J., Determination of key metabolites during biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine with Rhodococcus sp. strain DN22. Appl. Environ. Microbiol. 68, 166-172 (2002).
Dodard S. G., Renoux A. Y., Hawari J., Ampleman G., Thiboutot S., Sunahara, G. I., Ecotoxicity characterization of dinitrotoluenes and some of their reduced metabolites. Chemosphere, 38, 2071-2079 (1999).
Simini M., Wentsel R. S., Checkai R. T., Phillips C. T., Chester N. A., Major M. A., Amos J. C., Evaluation of soil toxicity at Joliet army ammunition plant. Environ. Toxicol. Chem. 14, 623-630 (1995).
Won W. D., DiSalvo L. H., Ng J., Toxicity and Mutagenicity of 2,4,6-Trinitrotoluene and its microbial metabolites. Appl. Envrion. Microbiol. 1976, 31, 576–580.
R G Kuperman, R T Checkai, C T Phillips, M Simini, J S Anthony, Effects of nitramine explosive CL-20 on the soil microinvertebrate community in a sandy loam soil, NTIS: ADA586378, 2013.
Sylvie Rocheleau a, Bernard Lachance a, Roman G. Kuperman, Toxicity and uptake of cyclic nitramine explosives in ryegrass Lolium perenne. Environmental Pollution. 156, 199-206 (2008).
Robidoux P Y, Sunahara G I, Savard K, Berthelot Y, Dodard S, Martel M, Gong P, Hawari J, Acute and chronic toxicity of the new explosive CL-20 to the earthworm (eisenia-andrei) exposed to amended natural soils, Environmental toxicology and chemistry, 23( 4), 1026-1034, 2004.
Kuperman R G, Checkai R T, Simini M, Phillips C T, Anthony J S, Kolakowski J E, Davis E A, Toxicity of Emerging Energetic Soil Contaminant CL-20 to Potworm Enchytraeus crypticus in Freshly Amended or Weathered and Aged Treatments. Chemosphere, 62, 1282–1293, 2006.
Bharat Bhushan, Louise Paquet, Jim C Spain, and Jalal Hawari, Biotransformation of 2,4,6,8,10,12-Hexanitro-2,4,6,8,10,12- Hexaazaisowurtzitane (CL-20) by Denitrifying Pseudomonas sp. Strain FA1, Applied and environmental microbiology, 69(7), pp 5216–5221, 2003.
P F Pagoria, Synthesis of LLM-105, NTIS: UCRL-JC-117228, 1997.
P F Pagoria, A R Mitchell, R D Schmidt, R L Simpson, F Garcia, J W Forb, Synthesis, Scale-up and Characterization of 2,6-Diamino-3,5-dinitropyrazine-l-oxide (LLM-105), NTIS: UCRL-JC- 130518, 1998.
R K Weese, A K Burnham, H C Turner, T D Tran, Physical Characterization of RX-55-AE-5 A formulation of 97.5% LLM-105 and 2.5% Viton A, NTIS: DE2006877793, 2005.
D M Hoffman, K T Lorenz, B Cunningham, F Gagliardi, Formulation and Mechanical Properties of LLM-105 PBXs, NTIS: LLNL-CONF-402822, 2008
Li Haibo, Cheng Bibo, Li Hongzhen, etc. Synthesis of 2,6-Diamino-3,5-dinitropyrazine-1-oxide, Chinese Journal of Organic Chemistry, 27(1), 112-115, 2007.
Li Haibo, Cheng Bibo, Liu Shijun, Recrystallization and Properties of LLM-105, Chinese journal of energetic materials, 16(6), 686-692, 2008.
Deng Mingzhe, Zhou Jiewen, Wang Bozhou, YE Zhihu, Tian Zhanhuai, Preparation Improvement of LLM-105 Explosive, Chinese journal of energetic materials, 21(3), 294-296, 2013.
Felichesmi Selestini Lyakurwa, Xianhai Yang, Xuehua Li, Xianliang Qiao, Jingwen Chen, Development of in silico models for predicting LSER molecular parameters and for acute toxicity prediction to fathead minnow, Chemosphere, 108,17–25, 2014.
Mei-Juan Wang, Xiao-Bo Zhao, Dan Wu, Ying-Qian Liu, Yan Zhang, Xiang Nan, Huanxiang Liu, Hai-Tao Yu, Guan-Fang Hu, and Li-Ting Yan, Design, Synthesis, Crystal Structure, Insecticidal Activity, Molecular Docking, and QSAR Studies of Novel N3-Substituted Imidacloprid Derivatives, Journal of Agricultural and Food Chemistry, 2014, 62, 5429−5442
Zhang Hui, Li Na, Ma Mei, Liu Guangbin, Quantitative Structure-Activity Relationship for Toxicity of Fifteen Substituted Phenols to Vibrio qinghaiensis sp.-Q67, Asian Journal of Ecotoxicology, 7(4), 373-380, 2012.
Huiping Zhao, Elisabetta Moroni, Bin Yan, Giorgio Colombo, and Brian S. J. Blagg, 3D QSAR Assisted Design, Synthesis, and Evaluation of Novobiocin Analogues, ACS Medicinal Chemistry Letters, 4, 57−62, 2013.
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