Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile
International Journal of Ecotoxicology and Ecobiology
Volume 1, Issue 2, September 2016, Pages: 60-66
Received: May 24, 2016; Accepted: Jul. 12, 2016; Published: Aug. 26, 2016
Views 3307      Downloads 99
Authors
Khaled Mohammed-Geba, Department of Zoology, Faculty of Science, Menoufia University, 32511 Shebin El-Kom, Egypt
Aldoushy A. Mahdy, Department of Zoology, Faculty of Science, Al-Azhar University, Assiut branch, 71524 Assiut, Egypt
Ahmed S. A. Eissa, Department of Fisheries, Organization of World Fish, Aswan, Egypt
Alaa G. M. Osman, Department of Zoology, Faculty of Science, Al-Azhar University, Assiut branch, 71524 Assiut, Egypt
Article Tools
Follow on us
Abstract
Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.
Keywords
Africa, COI, PCR, Population Genetics, Pufferfish, Tetraodon lineatus
To cite this article
Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman, Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile, International Journal of Ecotoxicology and Ecobiology. Vol. 1, No. 2, 2016, pp. 60-66. doi: 10.11648/j.ijee.20160102.15
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Akinyi, E., Azeroual, A., Bousso, T., Getahun, A. and Lalèyè, P. 2010. Tetraodon lineatus. The IUCN Red List of Threatened Species 2010: e. T 181807A7740095.
[2]
Santini, F., and Tyler J. C. 2003. A phylogeny of the families of fossil and extant tetraodontiform fishes (Acanthomorpha, Tetraodontiformes), Upper Cretaceous to Recent. Zoological Journal of the Linnean Society, 139: 565-617.
[3]
Tavani, G., 1955. Osservazioni su alcuni Plectognathi (Gymnodonti). Atti della Societa` Toscana di Scienze Naturali, Memorie, Serie A 62, 177–200.
[4]
Fouda, F. M., 2005. Anti-tumor activity of tetrodotoxin extracted from the Masked Puffer fish Arothron diadematus. Egyptian Journal of Biology, 7: 1-13.
[5]
Farrag, M., El-Haweet, A. A., and Moustafa, M. A. 2016. Occurrence of puffer fishes (Tetraodontidae) in the eastern Mediterranean, Egyptian coast-filling in the gap. BioInvasions Record, 5 (1): 47-54.
[6]
Gaillard, C., 1923. Recherches sur les poissons representes dans quelques tombeaux Egyptiens de l'ancien empire. Mémoires de l'Institut français d'archéologie orientale du Caire, 51: 97-100.
[7]
Halstead, B. W., and Lively Jr, W. M. 1954. Poisonous Fishes and Ichthyosarcotoxism. Their Relationship to the Armed Forces. United States Armed Forces medical journal, 5 (2): 157-75.
[8]
Wink, M., 1998. A short history of alkaloids. In Alkaloids (pp. 11-44). Springer US.
[9]
Zaki, A. M. 2004. Tetrodoxin poisoning associated with eating puffer fish in Suez City (Egypt). In: First International Conference on Natural Toxins, October 6 University, 18-19 December 2004– Egypt, pp 72.
[10]
Arakawa, O., Hwang, D. F., Taniyama, S., and Takatani, T., 2010. Toxins of Pufferfish That Cause Human Intoxications. Coastal Environmental and Ecosystem Issues of the East China Sea, Eds., A. Ishimatsu and H.-J. Lie, 227–244.
[11]
Beköz, A. B., Beköz, S., Yilmaz, E., Tüzün, S., and Beköz, U. 2013. Consequences of the increasing prevalence of the poisonous Lagocephalus sceleratus in southern Turkey. Emergency Medicine Journal, 30 (11): 954 955.
[12]
Souissi, J. B., Rifi, M., Ghanem, R., Ghozzi, L., Boughedir, W., and Azzurro, E. 2014. Lagocephalus sceleratus (Gmelin, 1789) expands through the African coasts towards the Western Mediterranean Sea: a call for awareness. Management of Biological Invasions, 5 (4): 357-362.
[13]
Smith, J. Kevin, and Kirk W. Barron 1990. Cardiovascular effects ofl-glutamate and tetrodotoxin microinjected into the rostral and caudal ventrolateral medulla in normotensive and spontaneously hypertensive rats. Brain Research, 506 (1): 1-8.
[14]
Nieto, F. R., Cobos, E. J., Tejada, M. Á., Sánchez-Fernández, C., González-Cano, R., and Cendán, C. M. 2012. Tetrodotoxin (TTX) as a therapeutic agent for pain. Marine Drugs, 10 (2): 281-305.
[15]
Bishai, H. M.; Khalil, M. T. 1997: Freshwater Fishes of Egypt. Publication of National Biodiversity Unit 9. Cairo.
[16]
Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R. and Hebert, P. D. 2005. DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society. Biological Sciences, 360 (1462): 1847-1857.
[17]
Ewing, B., Hillier, L., Wendl, M. C., and Green, P. 1998. Base-calling of automated sequencer traces using Phred. I. Accuracy assessment. Genome Research, 8 (3): 175-185.
[18]
Kumar, S., Stecher, G., and Tamura K., 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7): 1870–1874.
[19]
Rozas, J., Sánchez-DelBarrio, J. C., Messeguer, X. and Rozas R., 2003. DnaSP. DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19 (18): 2496-2497.
[20]
Excoffier, L., and Lischer, H. E., 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10 (3): 564-567.
[21]
Fu, Y. X., 1997. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 147 (2): 915-25.
[22]
Borrell, Y. J., Piñera, J. A., Sánchez Prado, J. A. and Blanco, G. 2012. Mitochondrial DNA and microsatellite genetic differentiation in the European anchovy Engraulis encrasicolus L.. ICES Journal of Marine Science; 69 (8): 1357 1371.
[23]
Harpending, H. C., 1994. Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution. Human Biology, 66: 591 600.
[24]
Ward, R. D., Woodwark, M., and Skibinski, D. O. F., 1994. A comparison of genetic diversity levels in marine, freshwater, and anadromous fishes. Journal of Fish Biology, 44 (2), 213-232.
[25]
Cooke, G. M., Chao, N. L., & Beheregaray, L. B., 2012. Natural selection in the water: freshwater invasion and adaptation by water colour in the Amazonian pufferfish. Journal of evolutionary biology, 25 (7): 1305-1320.
[26]
White, G. F., 1988. The environmental effects of the high dam at Aswan Environment. Science and Policy for Sustainable Development, 30 (7): 4-40.
[27]
Darwall, W., Smith, K., Allen, D., Holland, R., Harrison, I., Brooks, E., 2008. The diversity of life in African freshwaters: underwater, under threat. An analysis of the status and distribution of freshwater species throughout mainland Africa (No. 333.95096 D618div). Universidad de El Salvador, San Salvador (El Salvador).
[28]
Yamanoue, Y., Miya, M., Doi, H., Mabuchi, K., Sakai, H., and Nishida, M., 2011. Multiple invasions into freshwater by pufferfishes (Teleostei: Tetraodontidae): a mitogenomic perspective. PLoS One, 6 (2): e 17410.
[29]
Carnevale, G., and Tyler, J. C., 2015. A new pufferfish (Teleostei, Tetraodontidae) from the Middle Miocene of St. Margarethen, Austria. Paläontologische Zeitschrift, 89 (3): 435-447.
[30]
Guiraud, R., Bosworth, W., Thierry, J., Delplanque, A., 2005. Phanerozoic geological evolution of Northern and Central Africa: An overview. Journal of African Earth Sciences, 43: 83–143.
[31]
Vonhof, H. B., Wesselingh, F. P., Kaandorp, R. J. G., Davies, G. R., van Hinte J. E., Guerrero, J., Rasanen, M., Romero-Pittman, L., and Ranzi, A., 2003. Paleogeography of Miocene Western Amazonia: isotopic composition of molluscan shells constrains the influence of marine incursions. Geological Society of America Bulletin, 115: 983–993.
[32]
Lovejoy, N. R., Albert, J. S., Crampton, W. G. R., 2006. Miocene marine incursions and marine/freshwater transitions: Evidence from Neotropical fishes. Journal of South American Earth Sciences, 21: 5–13.
[33]
Wilson, A. B., Teugels, G. G., and Meyer, A. 2008. Marine incursion: the freshwater herring of Lake Tanganyika are the product of a marine invasion into West Africa. PLoS ONE 3: e 1979.
[34]
Santini, F., Nguyen, M. T. T., Sorenson, L., Waltzek, T. B., Lynch Alfaro, J. W., Eastman, J. M., and Alfaro, M. E., 2013. Do habitat shifts drive diversification in teleost fishes? An example from the pufferfishes (Tetraodontidae). Journal of Evolutionary Biology, 26 (5): 1003-1018.
[35]
Igarashi, Y., Doi, H., Yamanoue, Y., Kinoshita, S., Ishibashi, T., Ushio, H., Asakawa, S., Nishida, M., and Watabe, S., 2013. Molecular phylogenetic relationship of Tetraodon pufferfish based on mitochondrial DNA analysis. Fisheries science, 79 (2): 243-250.
[36]
Briggs, J. C., 2005. The biogeography of otophysan fishes (Ostariophysi: Otophysi): a new appraisal. Journal of Biogeography, 32 (2): 287–294.
[37]
Stewart, K. M., 2003. Fossil fish remains from Mio-Pliocene deposits at Lothagam, Kenya. Lothagam, the Dawn of Humanity in Eastern Africa, 3: 76-111.
[38]
Feibel, C. S., 1993. Freshwater stingrays from the Plio-Pleistocene of the Turkana Basin, Kenya and Ethiopia. Lethaia, 26 (4): 359-366.
[39]
Feibel, C. S., 2011. A geological history of the Turkana Basin. Evolutionary Anthropology: Issues, News, and Reviews, 20 (6): 206-216.
[40]
Drake, N., and Bristow, C., 2006. Shorelines in the Sahara: geomorphological evidence for an enhanced monsoon from palaeolake Megachad. The Holocene, 16 (6): 901-911.
[41]
Greenwood, P. H., 1951. CXI.—Fish remains from Miocene deposits of Rusinga Island and Kavirondo Province, Kenya. Journal of Natural History, 4 (48): 1192-1201.
[42]
Danley, P. D., Husemann, M., Ding, B., DiPietro, L. M., Beverly, E. J., and Peppe, D. J., 2012. The impact of the geologic history and paleoclimate on the diversification of East African cichlids. International Journal of Evolutionary Biology, 2012, doi: 10.1155/2012/574851.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186