International Journal of Microbiology and Biotechnology
Volume 2, Issue 3, August 2017, Pages: 116-120
Received: Dec. 30, 2016;
Accepted: Feb. 24, 2017;
Published: Mar. 21, 2017
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Sushil Kumar Barolia, Cytogenetic & Endocrinology Research Laboratory Department of Zoology, College of Science, M. L. Sukhadia University, Udaipur Rajasthan, India
Preeti Singh, Cytogenetic & Endocrinology Research Laboratory Department of Zoology, College of Science, M. L. Sukhadia University, Udaipur Rajasthan, India
Deepak Kumar Sharma, Department of Microbiology C. V. A. S. Navaniya, Vallabhanagar, Udaipur, Rajasthan, India
Previous and ongoing studies have incriminated bats as reservoirs of several emerging and re-emerging zoonoses. Most of these studies, however, have focused on viral agents and neglected important bacterial pathogens. To date, there has been no report investigating the prevalence of Burkholderia multivorans spp. in bats. The Burkholderia genus, being the largest, consists of Gram-negative, forms part of the Burkholderia complex, a group of Gram negative organisms which are commonly found in soil and water. And can survive for prolonged periods in moist environments. These bacteria can act as a powerful pesticide, capable of eliminating many soil-borne plant pathogens. Many species of Burkholderia are of considerable economic importance as these serve as insecticides, cause food poisoning, produce antibiotics etc. Hence in the present study an effort has been made to elucidate the presence of Burkholderia multivorans BPSS isolated, characterized and identified from the faeces of Pteropus giganteus from Udaipur, Rajasthan India. Its phylogenetic tree has also been derived, which showed evolutionary relationship of eleven related taxa. This is the first report from Indian subcontinent correlating the role of this megachiropteran as a carrier of Burkholderia multivorans BPSS.
Sushil Kumar Barolia,
Deepak Kumar Sharma,
Molecular Characterization and Identification of Burkholderia Multivorans BPSS Isolated from Fecal Contents of Pteropus Giganteus in Udaipur, Rajasthan, India, International Journal of Microbiology and Biotechnology.
Vol. 2, No. 3,
2017, pp. 116-120.
Adesiyun, A. A. (2009) Isolation of enteric pathogens from bats in Trinidad. Journal of Wildlife Diseases, 45 (4): 952–961.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990; 5; 215 (3):403–410.
Aneja KR. Experiments in microbiology, plant pathology and Biotechnology. New age international publication. (4th) Edition New Delhi, India; 2003.
Bergey's DH. Bergey's manual of Determinative Bacteriology 1105-1139; 1939.
Calisher CH. recent recognition of bats as reservoir hosts of emerging. Journal of Infection. 2006; 26:149-155.
Chhabra, M. B. and Pathak, K. M. L. (2008) Journal of parasiticdisease. 3 (2): 77-86.
Clare EL, Fraser EE, Braid HE, Fenton MB, Herbert PDN. Species on the menu of a generalist predator, the eastern red bat (Lasiurus borealis): using a molecular approach to detect arthropod prey. Molecular Ecology (2009); (18): 2532-2542.
Constantine DG. Transmission of pathogenic organisms by vampire bats. Natural history of vampire bats, Boca Raton, Florida, USA, CRC Press; 1988; 167-189.
Constantine, D. G., Green hall, A. M., Schmidt, U. (1988) Transmission of pathogenic microorganisms by vampire bats. Natural History of Vampire Bats Descriptors: rabies, reviews, vectors, transmission, parasites, 167-189.
Cox, P. A., Elmqvist, T., Pierson, E. D. and Rainey, W. E. (1991) Flying foxes as strong inter actors in south Pacific island ecosystems: a conservation hypothesis. Conservation Biology, 5 (4): 448-454.
Darryl, J. H. Jennifer., Young, L. D., Barbara Goodyear, B. S., and Georgeann, A. Ellis., B. S. (1997) Comparative rectal bacterial flora of four species of flying fox (Pteropus sp.). Journal of Zoo and Wildlife Medicine, 28 (4): 471-475.
Felsenstein J. Confidence limits on phylogenies an approach using the bootstrap. Evolution1985; 39: 783-791.
Ferdinand JC. Studies on bacteria. Contributions to the biology of plant No. 2, 1872; 1: 127-224.
Fleming, T. H. (1986) Opportunism versus specialization: the evolution of feeding strategies in frugivorous bats. 105-118.
Fleming, T. H. (1988) The short-tailed fruit bat: A study in plant animal interactions. University of Chicago Press, Chicago, Illinois.
Hasebe F Mai. Surveillance of bats as reservoir host of emerging zoonotic in Vietnam. Tropical Medicine and Health. 2007; 35: 51-53.
Hill, J. E. and Smith, J. D. (1984) Bats: a natural history. University of Texas press, Austin, Texas, pp.243.
Klite, P. D. (1965) Intestinal bacterial flora and transit time of three Neotrophical bat species. J Bacteriol. 90: 375-379.
Klite, P. D. (1965) Isolation of Salmonella from a Neotrophical bat. J Bacteriol., 90: 831.138
Klite, P. D. and Dierks, F. H. (1965) Histoplasma capsulatum in fecal contents and organs of bat in the canal Zone- Amer. J trop Med Hyg., 14: 433-439.
Kimura M. A Simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. 1980; 16: 111-120.
Pierson, E. D. and Rainey, W. E. (1992) the biology of flying foxes of the genus Pteropus: a review. Pacific Island flying foxes: proceedings of an international conservation conference. U.S. 90: 1-72.
Pinus M, Muller HE. Enterobacteria of bat (Chiroptera, Zentralbl, Bakteriol A. 1980; 247 (3): 315 -322.
Preeti S, Barolia SK, Deora K, Mogra P, Bano H, Javeria S. Studies on gut microbial parasites from faecal contents of Pteropus giganteus: short review. World Journal of Environmental Biosciences 2012; 1 (1): 5-8.
Saitou N, Nei M. The neighbor-joining method a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 1987; (4): 406-425.
Servat, A., Herr, J., Picard-Meyer, E., Schley, L., Harbusch, C., Michaux, C., Pir, J., Robardet, E., Engel, E., Cliquet, F. and Meyer Frist. (2015) isolation of Rabid Bat infected with European Bat lyssa virus in Luxembourg.
Singh P, Barolia SK, Sharma DK. Identification, isolation and characterization of Enterococcus species (GenBank accession no: KF777815) from fecal contents of Pteropus giganteus International Journal of Current Microbiology and Applied Sciences 2015; 4 (3): 143-149.
Singh P, Barolia SK, Sharma DK. Isolation, Characterization and Identification of Bacillaceae bacterium spp. (GenBank Accession No: KF777817) from Fecal Contents of Pteropus giganteus in Udaipur, Rajasthan, India. International Journal of Advanced Research 2015; 3: (8): 331-335.
Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0: Molecular Biology and Evolution 2007; (24): 1596-1599.
Turnbull P, Kramer J, Melling. J Bacillus In: Topley and Wilson Principles of Bacteriology, Virology and Immunity 8th ed Edward Arnold, London pp. 1990; 185-210.
Wong S, Lau S, Woo, P, Yuen KY. Bats as a continuing source of emerging infections in humans. (Rev Med Viro) 2007; (17): 67-91.