Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria
International Journal of Microbiology and Biotechnology
Volume 5, Issue 2, June 2020, Pages: 48-54
Received: Feb. 7, 2020; Accepted: Feb. 21, 2020; Published: Apr. 21, 2020
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Authors
Florence Zion Uyanga, Department of Microbiology, Akwa Ibom State University, Mkpat Enin, Nigeria
Emmanuel Olufemi Ekundayo, Departments of Microbiology, Michael Okpara University of Agriculture, Umudike, Nigeria
Emmanuel Onwubiko Nwankwo, Departments of Microbiology, Michael Okpara University of Agriculture, Umudike, Nigeria
Inimfon Akaninyene Ibanga, Department of Microbiology, Akwa Ibom State University, Mkpat Enin, Nigeria
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Abstract
Extended spectrum beta-lactamase prevalence is still on the increase across the world and has been implicated in urinary tract infections in Nigeria. TEM, SHV and CTX-M are becoming more common with CTX-M-15 becoming more significant as it is associated with complicated urinary tract infections. This study was conducted to evaluate distribution of blaTEM, blaCTX-M-15 and blaSHV genes among Enterobacter cloacae, E. coli and Klebsiella pneumoniae. A total of 660 urine samples were collected from pregnant women in 3 general hospitals from Akwa Ibom state. Enterobacter cloacae, E. coli and Klebsiella pneumoniae were identified using Microbact 24E. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility. The presence of ESBL was detected using Double Disk Synergy Test (DDST) and CHROMagar ESBL, respectively. Plasmid extraction was carried out following the protocol of ZR Plasmid Miniprep-Classic extraction kit. The blaCTX-M-15, blaTEM and blaSHV was identified by PCR with specific primers in selected 50 ESBL producing isolates. A total of 252 clinical isolates were collected from three General Hospitals in South-South, Nigeria. ESBLs were found in 231 (92%) isolates. blaCTX-M-15 was the commonest genotype (84%), followed by blaSHV (60%) and blaTEM (60%). ESBL positive strains of E. cloacae, E. coli and K pneumoniae are increasingly found in isolates from pregnant women. The current study demonstrated the predominance of gene encoding blaCTX-M-15 with a percentage frequency of (84%) commonly with plasmid in the setting.
Keywords
ESBL blagene, PCR, UTI
To cite this article
Florence Zion Uyanga, Emmanuel Olufemi Ekundayo, Emmanuel Onwubiko Nwankwo, Inimfon Akaninyene Ibanga, Molecular Characterization of Extended Spectrum Beta-lactamase from Enterobacter cloacae, E. coli and Klebsiella pneumoniae from Pregnant Women in South-south Nigeria, International Journal of Microbiology and Biotechnology. Vol. 5, No. 2, 2020, pp. 48-54. doi: 10.11648/j.ijmb.20200502.12
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Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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