International Journal of Microbiology and Biotechnology
Volume 4, Issue 4, December 2019, Pages: 128-132
Received: Aug. 22, 2019;
Accepted: Sep. 11, 2019;
Published: Oct. 23, 2019
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Prajakta Naval, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India
Chandra Sainathan, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India
A κ-carrageenan degrading bacterium was isolated from decaying red seaweeds. Based on biochemical characterization and 16S rRNA sequence the isolate was identified as Alteromonas macleodii KS62. To the best of our knowledge, this is the first study reporting κ-carrageenan degrading bacterium of species Alteromonas macleodii. κ-carrageenase enzyme production of 1.6U/ml (12.5U/mg protein) was obtained when Alteromonas macleodii KS62 was grown in a medium containing κ-carrageenan as carbon source. The κ-carrageenase production further increased to 2.9U/ml (14.1U/mg protein) when Alteromonas macleodii KS62 was grown in a medium supplemented with Kappaphycus red seaweed powder. This the first report on κ-carrageenase production using red seaweed biomass as substrate. Efficient κ-carrageenase production establishes the potential of red seaweed biomass to serve as a low cost substrate for economic production of κ-carrageenase enzymes. Enzymatic hydrolysis of κ-carrageenan was studied. κ-carrageenan tetrasaccharide and hexasaccharide were found to be the major end products of enzymatic hydrolysis.
Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism, International Journal of Microbiology and Biotechnology.
Vol. 4, No. 4,
2019, pp. 128-132.
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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