Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal
International Journal of Microbiology and Biotechnology
Volume 3, Issue 1, March 2018, Pages: 25-30
Received: Jan. 17, 2018;
Accepted: Mar. 19, 2018;
Published: Apr. 10, 2018
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Kamal Rai, Department of Biological Protein Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P. R. China
Sujan Khadka, Department of Life Sciences, Biochemistry and Molecular Biology, Central China Normal University, Wuhan, P. R. China
Bidya Shrestha, Department of Microbiology, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal
With the objective to isolate, identify and determine the antimicrobial activity of the soil actinomycetes, this study was done in the laboratory of Nepal Polytechnic Institute, Chitwan (September 2015- April 2016). A total of 30 soil samples were randomly collected and processed from forest, garden, cultivated lands and river side (Narayani and Rapti River) of Chitwan, Nepal. Isolates were identified by colony characterizations, microscopy and biochemical tests. Primary and secondary screening for antibiotic producers was done by single line cross streak and agar cup assay respectively against test organisms, S. Typhi, S. aureus and E. coli. Altogether 25 different actinomycetes from two genera viz; Streptomyces (n=15) and Micromonospora (n=10) were identified from selected sites (n=10; river side, n=5 each from garden, cultivated and forest soil). Primary screening of n=12 isolates was done by inhibition to at least one test bacterium. Of them only n=9 (n=6 Streptomyces spp., n=3 Micromonospora spp.) isolates showed promising antibacterial activity (zone of inhibition above 8mm) on secondary screening. Maximum zone of inhibition was 14mm observed against S. aureus and whereas 18mm against E. coli and S. Typhi respectively. This finding indicates potent antibiotic producer actinomycetes were isolated from soil of Chitwan, study furthering strain selection and identification would pave the way for commercial antibiotic production.
Actinomycetes: Isolation, Characterization and Screening for Antimicrobial Activity from Different Sites of Chitwan, Nepal, International Journal of Microbiology and Biotechnology.
Vol. 3, No. 1,
2018, pp. 25-30.
Agarawal VP “Micorbial Biodiversity of Khumbu region: Study of antibiotic activity of Actinomycetes”, http://www.evk2cnr.org/files/PAgrawal01.pdf.
Magarvey NA, Keller JM, Bernanetal V. Isolation and characterization of novel marine derived Actinomycetes taxa rich in bioactive metabolites. Applied and Environmental Microbiol. 2004; 70: 7520-7529.
Okami Y, Hotta K. Search and discovery of new antibiotics”, In: Actinomycetes in biotechnology (Eds. M. Good Fellow, S. T. Williams and M. Mordarski). Academic press, London1988: 37-67.
Sathi ZS, Md AA Rahman, Gafur MA. Identification and in vitro anti-microbial activity of a compound isolated from Streptomyces species. Pakistan J Biol Sci. 2001; 4 (12): 1523-1525.
Zhao H, Parry RL, Ellis DI, Griffith GW, Goodacre R. The rapid differentiations of Streptomyces using fourier transform infrared spectroscopy. Vibrat Spectr. 2006; 40: 213–218.
Valli S, Suvathi SS, Aysha OS, Nirmala P, Vinoth P, Reena A. Antimicrobial potential of Actinomycetes species isolated from marine environment. Asian Pac J Trop Biomed. 2012; 6: 469-473.
Nathan AM, Jessica MK, Valerie B, Martin D, David HS. Isolation and Characterization of Novel Marine-Derived Actinomycete Taxa Rich in Bioactive Metabolites. Appl. Environ. Microbiol. 2004; 70 (12): 7520-7529.
Sengupta S, Pramanik A, Ghosh A, Bhattacharyya M. Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem. BMC Microbiol. 2015; 15: 170.
Rahman A, Islam MZ, Islam AUI. Antibacterial Activities of Actinomycete Isolates Collected from Soils of Rajshahi, Bangladesh. Biotechnol Res Int’l. 2011 (2011): 1-6.
Gurung TD, Sherpa C, Agrawal VP, Lekhak B. Isolation and characterization of antibacterial Actinomycetes from soil samples of Kalapatthar, Mount Everest Region. Nepal J Sci Technol. 2009; 10: 173-182.
Bizuye A, Moges F, Andualem B. Isolation and screening of antibiotic producing Actinomycetes from soils in Gondar town, North West Ethiopia. Asian Pac J Trop Dis 2013; 3 (5): 375-381.
Panwar D, Vipin SK. Antagonistic activity of soil actinomycetes against common Human pathogens. Int J Pharm Bio Sci, 2012; 4: 500-506.
Attimarad SL, Ediga GN, Karigar AA, Karadi R, Chandrashekhar N, Shivanna C. Screening, isolation and purification of antibacterial agents from marine Actinomycetes. Int’l Current Pharmaceut J. 2012; 1 (12): 394-402.
Ceylan O, Okmen G, Ugur A. Isolation of soil Streptomyces as source antibiotics active against antibiotic-resistant bacteria. Eur Asia J Biol Sci. 2008; 2 (9): 73-82.
Rana S, Salam MD. Antimicrobial Potential of Actinomycetes Isolated from Soil Samples of Punjab, India. J Microbiol Exp. 2014; 1 (2): 00010.
Padalkar RR, Peshwe SA. Isolation and characterization of new anti-MRSA producing Actinomycete. Indian J App Res. 2013; 3 (6): 433-436.
Thenmozhi M, Kannabiran K. Studies on isolation, classification and phylogenetic characterization of novel antifungal Streptomyces sp. VITSTK7 in India. Curr Res J Biol Sci. 2010; 2 (5): 306-312.
Selvameenal L, Radhakrishnan M, Balagurunathan R. Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. Indian J Pharm Sci. 2009; 71 (5): 499-504.
Alanis AJ. Resistance to antibiotics: are we in the post-antibiotic era. Archives of Medical Research. 2005; 36 (6): 697-705, 2005.
Abo-Shadi M. Abd. AR, Sidkey NM, Al-Mutrafy AM. Antimicrobial agent producing microbes from some soils rhizosphere. J Am Sci, 2010; 6 (10): 915-925.
Khandan ND, Janardhana GR. Diversity and Antimicrobial activities of Actinomycetes isolated from soil samples of Kodagu, Karnataka State (India). Int’l J Microbiol Res. 2013; 5: 404-409.
Gopala krishnan S, Sunder J, Sasidharan V, Subramanian SE. Antibacterial activity of actinobacteria isolated from mangroves of Andaman and Nicobar Islands, India. Adv. Anim. Vet. Sci. 2016; 5: 230-236.
Pathalam G, Appadurai DR, Rajendran HAD, Munusamy RG, Michael GP, Naif AAD, Savarimuthu I. Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India. Alexandria Journal of Medicine. 2017; 53: 101–110.
Oskay M. Antifungal and antibacterial compounds from Streptomyces strains. African Journal of Biotechnology. 2009; 8 (13): 3007-3017.
Kumar N, Singh RK, Mishra SK, Singh AK, Pachouri UC. Isolation and screening of soil Actinomycetes as source of antibiotics active against bacteria. Int’l J of MicrobiolRes. 2010; 2 (2): 12-16.
Kumari M, Myagmarjav BE, Prasad B, Choudhary M. Identification and Characterization of antibiotic-producing Actinomycetes isolates. Amer J of Microbiol. 2013; 4 (1): 24-31.
Emelda EJ, Vijayalakshmi N, Santhanakrishnan T. Antimicrobial activity of antibiotic producing Streptomyces macrospores. J of Pharma and Biol Sci. 2012; 2 (3): 20-23.
Jeffrey LSH. Isolation, characterization and identification of actinomycetes from agriculture soils at Semongok, Sarawak. Afr J of Biotech. 2008; 7 (20): 3697-3702.
Vijayakumar R, Panneer SK, Muthukumar C, Thajuddin A, Panneerselvam A, Saravanamuthu R. Antimicrobial potentiality of a halophilic strain of Streptomyces sp. VPTSA18 isolated from saltpan environment of Vedaranyam, India. Ann Microbiol. 2012; 62: 1039–47.
Saravanakumar P, Preetjam Raj JP, Duraipandiyan V, Ignacimuthu S. Antibacterial activity of some actinomycetes from Tamil Nadu, India. Asian Pac J Trop Biomed. 2012; 2:936–46.