Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages
International Journal of Microbiology and Biotechnology
Volume 3, Issue 1, March 2018, Pages: 11-24
Received: Jan. 10, 2018; Accepted: Feb. 1, 2018; Published: Mar. 23, 2018
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Agnes Njoki Mwaura, Department of Biochemistry, University of Nairobi, Nairobi, Kenya
Betty Nyambura Mbatia, School of Pharmacy and Health Sciences, United States International University - Africa, Nairobi, Kenya
Edward Kirwa Muge, Department of Biochemistry, University of Nairobi, Nairobi, Kenya
Patrick Wafula Okanya, Department of Biochemistry and Biotechnology, Technical University of Kenya, Nairobi, Kenya
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Release of petroleum oil and its products into the environment is a worldwide concern. The present study focused on isolation, molecular identification, morphological and biochemical characterization of bacteria possessing hydrocarbon-degrading properties. The study also aimed at optimizing appropriate culture conditions for the isolates as well as screening for alkane hydroxylase enzyme. Out of twenty one microbes isolated, nine were selected based on their ability to utilize different hydrocarbons. The isolates were observed to mineralize heating oil, hexane, octane, toluene and diesel oil. PCR amplification of 16S rDNA gene revealed that the isolates belong to six different genera; Pseudomonas, Acinetobacter, Klebsiella, Enterobacter, Salmonella and Ochrobactrum. Based on their ability to degrade diesel oil, three isolates were selected and their growth conditions optimized. Optimum degradation was recorded at less than 1% substrate concentration, pH 7 and temperature range of between 30°C and 37°C and using yeast extract as nitrogen source. GC-MS analyses confirmed their diesel oil degrading properties. Alkane hydroxylase gene from one of the isolates (isolate 1C) was successfully amplified indicating its catabolic capabilities in degrading alkanes. Overall, the characterized isolates may constitute potential candidates for biotechnological application in environmental cleanup of petroleum contaminants.
Oil-Contaminated Soils, Bacteria, Biodegradation, Petroleum Hydrocarbons
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Agnes Njoki Mwaura, Betty Nyambura Mbatia, Edward Kirwa Muge, Patrick Wafula Okanya, Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages, International Journal of Microbiology and Biotechnology. Vol. 3, No. 1, 2018, pp. 11-24. doi: 10.11648/j.ijmb.20180301.13
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