Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests
International Journal of Microbiology and Biotechnology
Volume 2, Issue 4, November 2017, Pages: 156-160
Received: Apr. 27, 2017; Accepted: May 22, 2017; Published: Jun. 16, 2017
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Irina Marinova Gotova, R&D Center LB Bulgaricum Plc, Sofia, Bulgaria
Zhechko Panayotov Dimitrov, R&D Center LB Bulgaricum Plc, Sofia, Bulgaria
Hristo Miladinov Naidenski, The Stefan Angelov Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
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Lactobacillus gasseri G4 strain isolated from healthy human volunteer was selected as one of the best adhesive strain among a large group of lactobacilli. In-vitro tests for establishment of immunomodulatory potential of selected lactic acid bacteria were carried out. Three different analytical models were used for evaluation, according to the specifics of each different cytokine and to compare, where possible. The induction of cytokines like: interleukin-1 (IL-1), IL-10 and tumor necrosis factor alpha (TNF-α) were determined by widely used laboratory approach with mouse splenocytes as well as on combined (well-in-well) monocytic and epithelium human cell lines (THP-1 and HT-29). Other cytokines like IL-8 and IL-4 were evaluated by only one analytical model, while transforming growth factor beta (TGF-β) was evaluated by using all of the three models. The selected strain shows significant increase of IL-10, interferon gamma (IFN-γ) and TGF-β production in-vitro. Therefore, this strain was directed to clinical trials with 38 healthy volunteers, randomized and placebo-controlled. The results demonstrate statistically significant increase of TGF-β production (25%). Immunoglobulin A (IgA) and IFN-γ were also increased. Stable levels of IL-10 were observed after the in-vivo experiments with L. gasseri G4 strain. The selected strain does not increase in-vivo the pro-inflammatory cytokine IL-8. In this study the immunomodulatory properties of L. gasseri G4 were confirmed in-vivo therefore it is suitable for development of probiotic formulas.
Probiotics, Lactobacillus gasseri, Immunomodulation, Cytokines, Immunoglobulin
To cite this article
Irina Marinova Gotova, Zhechko Panayotov Dimitrov, Hristo Miladinov Naidenski, Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests, International Journal of Microbiology and Biotechnology. Vol. 2, No. 4, 2017, pp. 156-160. doi: 10.11648/j.ijmb.20170204.11
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