Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia
International Journal of Microbiology and Biotechnology
Volume 2, Issue 3, August 2017, Pages: 135-138
Received: Oct. 3, 2016; Accepted: Mar. 19, 2017; Published: Apr. 18, 2017
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Authors
Vladimir Mihajlovich Sorokin, Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Ruslan Vjacheslavovich Pisanov, Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Elena Vadimovna Golubkina, Department of Biochemistry, Astrakhan State Medical University, Ministry of Health of Russia, Astrahan', Russia
Elena Aleksandrovna Bereznyak, Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Lubov Aleksandrovna Prozorova, Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
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Abstract
High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.
Keywords
Helicobacter Pylori, VNTR Genotyping, MLVA, Cluster Analysis, Molecular Epidemiology
To cite this article
Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova, Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia, International Journal of Microbiology and Biotechnology. Vol. 2, No. 3, 2017, pp. 135-138. doi: 10.11648/j.ijmb.20170203.15
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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