Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites
International Journal of Microbiology and Biotechnology
Volume 2, Issue 3, August 2017, Pages: 129-134
Received: Mar. 4, 2017; Accepted: Mar. 18, 2017; Published: Apr. 18, 2017
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Authors
Iyobosa Benedicta Idahosa, Department of Biology, College of Education, Ekiadolor, Benin City, Nigeria
Henrietta Omokhele Obueh, Department of Biology, College of Education, Ekiadolor, Benin City, Nigeria
Millicent Omoyemhe Aigbekaen, Department of Biology, College of Education, Ekiadolor, Benin City, Nigeria
Eunice Asiboroko Odesiri - Eruteyan, Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Warri, Nigeria
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Abstract
Antimicrobial susceptibility and toxigenicity tests were carried out on bacterial species isolated from the soil samples collected from an urban market (Oba market) and a rural market (Ekiadolor market) waste dumpsites. The bacterial species included Escherichia coli, Shigella sp, Staphylococcus aureus, Salmonella sp, Bacillus sp, Enterococcus sp, Clostridium sp, Proteus sp, Klebsiella sp and Pseudomonas sp. E. coli had the highest percentage occurrence of 56.0% in the urban market waste dumpsite and Enterococcus sp had the highest percentage occurrence of 28.13% in the rural market waste dumpsite. Toxigenicity test was carried out on S. aureus, Salmonella sp and E. coli isolates to ascertain enterotoxin production using the rabbit ileal loop assay. S. aureus (60%), Salmonella sp (50%) and E. coli (62.5%) isolates were positive to enterotoxin production. Ciprofloxacin (CIP), Peflacin (PEF), Tetracycline (TET) and Gentamycin (GEN) were the most effective antibiotics against the bacterial isolates. Antimicrobial susceptibility of the bacterial isolates from the urban waste dumpsite was CIP (40.74%), PEF (35.19%) and GEN (33.33%) and antimicrobial susceptibility of the bacterial isolates from the rural waste dumpsite was CIP (51.43%), TET (40.00%) and GEN (42.86%). The antibiotics were more effective against the bacterial isolates from the rural market dump site. Pollution of the environment by wastes from markets is of public health concern because the presence of pathogenic bacteria in the wastes has the potential of contaminating air, soil and ground water.
Keywords
Bacterial Isolates, Dumpsites, Market, Pathogenic, Rural, Urban
To cite this article
Iyobosa Benedicta Idahosa, Henrietta Omokhele Obueh, Millicent Omoyemhe Aigbekaen, Eunice Asiboroko Odesiri - Eruteyan, Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites, International Journal of Microbiology and Biotechnology. Vol. 2, No. 3, 2017, pp. 129-134. doi: 10.11648/j.ijmb.20170203.14
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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