Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study
International Journal of Microbiology and Biotechnology
Volume 2, Issue 2, May 2017, Pages: 52-57
Received: Oct. 8, 2016; Accepted: Dec. 14, 2016; Published: Jan. 20, 2017
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Eman Zakaria Gomaa, Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Cairo, Egypt
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Introduction: Modern dentistry emphasizes the importance of dental plaque control to improve oral health. To that end the development of oral care formulations has been geared toward the incorporation of antiplaque agents that may play a crucial role in oral health maintenance. Aims: The aims of this work were to incorporate chitosan produced by Lactobacillus plantarum into a mouthwash matrix and assess its effect upon microbial adherence and biofilm formation of oral microorganisms. Additionally, the action of the chitosan mouthwash was compared with two commercially mouthwashes. Methods: A total of 38 lactic acid bacteria, belonging to Lactobacillus species, isolated from 24 samples of traditional Egyptian dairy products, were screened for chitin degradation. Lactobacillus plantarum is the best producer of the enzyme chitin deacetylase so as to release chitosan. Results: The chitosan containing mouthwashwes capable of interfering with all microorganisms’ growth, adherence and biofilm formation and showing vastly superior activity than both chitosan and commercial mouthwashes assayed. Conclusions: Chitosan mouthwashes show great potential as a natural and efficient alternative to traditional mouthwashes.
Shrimp Waste, Chitosan, Lactic Acid Bacteria, Anti-adherence, Biofilm Formation
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Eman Zakaria Gomaa, Improvement of Antimicrobial and Anti-biofilm Potentials of Mouthwashes by Chitosan Produced by Lactic Acid Bacteria: An in vitro Study, International Journal of Microbiology and Biotechnology. Vol. 2, No. 2, 2017, pp. 52-57. doi: 10.11648/j.ijmb.20170202.11
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T. F. Flemmig, T. Beikler, Control of oral biofilms. Periodontology, 2011, vol. 55 (1), pp. 9–15.
C. A. Franco Neto, C. C. Parolo, C. K. Rosing, M. Maltz, Comparative analysis of the effect of two chlorhexidine mouthrinses on plaque accumulation and gingival bleeding. Braz. Oral. Res., 2008, vol. 22, pp. 139-144.
R. Jayakumar, N. Nwe, S. Tokura, H. Tamura, Sulfated chitin and chitosanas novel biomaterials. Int. J. Biol. Macromol., 2007, vol. 40 (3), pp. 175–181.
E. M. Cost, S. Silva, M. R. Costa, M. Pereira, D. A. Campos, J. Odia, A. R. Madureira, A. Cardelle-Cobas, F. K. Tavaria, A. S. Rodrigues, M. M. Pintado, Chitosan mouthwash, pp. Toxicity and in vitro validation. Carbohyd. Polym., 2014; vol. 111, pp. 385-392.
A. A. Tayel, S. H. Moussa, K. Opwis, D. Knittel, E. Schollmeyer, S. Nickisch-Hartfiel. Inhibition of microbial pathogens by fungal chitosan. Int. J. Biol. Macromol., 2010; vol. 47, pp. 10–14.
E. M. Costa, S. Silva, C. Pina, F. K. Tavaria, M. M. Pintado, Evaluation and insights into chitosan antimicrobial activity against anaerobic oral pathogens. Anaerobe, 2012 vol. 18 (3), pp. 305-309.
M. E. Badawy, E. I. Rabea, Preparation and antimicrobial activity of o-(benzoyl) chitosan derivatives against some plant pathogens. Afr. J. Microbiol. Res., 2013, vol. 7 (20), pp. 2259-2268.
E. M. Costa, S. Silva, F. K. Tavaria, M. M. Pintado, Study of the effects of chitosan upon Streptococcus mutans adherence and biofilm formation. Anaerobe, 2013, vol. 20, pp. 27-31.
A. A. Rushdy, E. Z. Gomaa, Antimicrobial compounds produced by probiotic Lactobacillus brevis isolated from dairy products. Ann. Microbiol., 2013, vol. 63, pp. 81‒90.
R. S. Vadake, Biotransformation of chitin to chitosan, United States Patent 1998, 5739015.
H. J. Bader, E. Birkholz, Chitin Handbook, Atec Edizioni, Grottammare, Italy, 1997.
S. Ennahar, T. Zendo, K. Sonomoto, A. Ishizaki, Investigation of bacetriocin production and purification from Nukadoko isolates displaying anitimicrobial activity. Jpn. J. Lactic Acid Bact., 1999, vol. 10, pp. 29–36.
S. Stepanovic, D. Vukovic, I. Dakic, B. Savic, M. Svabic-Vlahovic, A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J. Microbiol. Meth., 2000 vol. 40 (2), 175–179.
S. N. Kulikov, Y. Shumkova, Effect of chitosan on lysostaphin lysis of staphylococcal cells. Bull. Exp. Biol. Med., 2014 vol. 157 (2), pp. 243-245.
K. Tokuyasu, M. Ohnishi-Kameyama, K. Hayashi, Purification and characterization of extracellular chitin deacetylase from Collecotrichum lindemuthianum. Biosci. Biotechnol. Biochem., 1996 vol. 60 (10), pp. 1598–1603.
S. J. Jeon, M. Oh, W. K. Yeo, K. N. Galvao, K. C. Jeong, Underlying mechanism of antimicrobial activity of chitosan microparticles and implications for the treatment of infectious diseases. PLoS One, 2013, vol. 9 (3), pp. 92723.
I. Younes, S. Sellimi, M. Rinando, K. Jellonli, M. Nasri, Influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities. Int. J. Food Microbiol., 2014, vol. 185, pp. 57-63.
D. Raafat, H. G. Sahl, Chitosan and its antimicrobial potential-a critical literature survey. Microb. Biotechnol., 2009, vol. 2 (2), pp. 186-201.
M. E. Frank, J. F. Gent, T. P. Hettinger, Effects of chlorhexidine on humantaste perception. Phys. Behav., 2001, vol. 74 (1-2), pp. 85–99.
W. Krzyściak, A. Jurczak, D. Kościelniak, B. Bystrowska, A. Skalniak, The virulence of Streptococcus mutans and the ability to form biofilms. Eur. J. Clin. Microbiol. Infect. Dis., 2014, vol. 33, pp. 499–515.
Q. X. Ji, D. Y. Zhong, R. Lu, W. Q. Zhang, J. Deng, X. G. Chen, In vitro evaluation of the biomedical properties of chitosan and quaternized chitosan for dental applications. Carbohyd. Res., 2009, vol. 344 (17), pp. 2279–2424.
M. J. Verkaik, H. J. Busscher, D. Jager, A. M. Slomp, F. Abbas, H. C. van der Mei, Efficacy of natural antimicrobials in toothpaste formulations against oral biofilm in vitro. J. Dent., 2011, vol. 39, pp. 218-224.
V. Archana, M. L. Prabhuji, B. V. Karthikeyan, A. Selvan, Control of Streptococcus sanguinis oral biofilm by novel chlorhexidine-chitosan mouthwash, pp. an in vitro study. J. Exp. Integr. Med., 2013, vol. 3 (2), pp. 165-169.
N. Cerca, S. Martins, G. B. Pier, R. Oliveira, J. Azeredo, The relationship between inhibition of bacterial adhesion to a solid surface by sub-mics of antibiotics and subsequent development of a biofilm. Res. Microbiol., 2005, vol. 156, pp. 650–655.
C. Hannig, M. Hannig, The oral cavity – A key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin. Oral. Invest., 2009, vol. 13 (2), pp. 123–139.
A. H. Nobbs, H. F. Jenkinson, N. S. Jakubovics, Stick to your gums. J. Dent. Res., 2011, vol. 90 (11), pp. 1271–1278.
P. D. Marsh, Controlling the oral biofilm with antimicrobials. J. Dent., 2010, vol. 38, pp. 11–15.
Z. Terefework, C. L. Pham, A. C. Prosperi, M. M. Entius, A. Errami, R. J. Spanning, MLPA diagnostics of complex microbial communities, pp. Relative quantification of bacterial species in oral biofilms. J. Microbiol. Meth., 2008, vol. 75 (3), 558–565.
L. Dong, Z. Tong, D. Linghu, Y. Lin, R. Tao, J. Liu, Effects of sub-minimum inhibitory concentrations of antimicrobial agents on Streptococcus mutans biofilm formation. Int. J. Antimicrob. Agent, 2012, vol. 39 (5), pp. 390–395.
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