Evaluation and Antimicrobial Susceptibility Pattern of Pathogenic Bacteria in Poultry Wastes
International Journal of Microbiology and Biotechnology
Volume 1, Issue 1, November 2016, Pages: 10-15
Received: Jul. 19, 2016; Accepted: Sep. 14, 2016; Published: Dec. 17, 2016
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Authors
Amechi Sampson Nwankwegu, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
Chima Victor Nzomiwu, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
Nwanneka Chinazor Nwozor, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
Herbert Chijioke Eneite, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
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Abstract
In the present study, the prevalence of pathogenic bacteria from poultry environment was investigated. Potentially pathogenic organisms were isolated and identified notably; Salmonella enteritidis, Shigella sp., Clostridium perfringens, Pseudomonas sp., E. coli, Staphylococcus aureus, Vibrio cholerae and Vibrio parahemolyticus. The susceptibility of the isolates to different antibiotics was tested and it was observed that Pseudomonas sp. was very sensitive to the antibiotics, Ceporex (10µg) and Tarivd (10µg) with 16mm zone of inhibition. Clostridium sp. was more susceptible to Levofloxacin (20µg) with 20 mm zone of inhibition, Staph. aureus was more susceptible to Streptomycin (30µg) and Levoflaxacin (20µg) with 18mm zone of inhibition, E. coli was more susceptible to Gentamycin (10µg) with 20mm zone of inhibition, Salmonella enteritidis was more sensitive to Augmentin (30µg) with 20mm zone of inhibition and Shigella sp. was more sensitive to Ciproflox (10µg) with 20mm zone of inhibition. The pathogenicity of these isolates was studied by infecting each on mice. There was death of two mice infected with Clostridium perfringens. Mice infected with Shigella sp., suffered swollen of the scrotum and scrotal sac which was observed after dissection. Mice infected with Pseudomonas sp., Staphylococcus aureus, and Salmonella enteritidis and E. coli, 25x108, 8x108, 20x108, 10x108 cfu/ml of the infected organisms were recovered from their intestine respectively. It seems that the organisms colonized their intestine at high level and they shed them in their faeces, though the infections were asymptomatic at the stage.
Keywords
Poultry, Prevalence, Pathogenic Bacteria, Sensitivity
To cite this article
Amechi Sampson Nwankwegu, Chima Victor Nzomiwu, Nwanneka Chinazor Nwozor, Herbert Chijioke Eneite, Evaluation and Antimicrobial Susceptibility Pattern of Pathogenic Bacteria in Poultry Wastes, International Journal of Microbiology and Biotechnology. Vol. 1, No. 1, 2016, pp. 10-15. doi: 10.11648/j.ijmb.20160101.12
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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