Evaluation and Antimicrobial Susceptibility Pattern of Pathogenic Bacteria in Poultry Wastes
International Journal of Microbiology and Biotechnology
Volume 1, Issue 1, November 2016, Pages: 10-15
Received: Jul. 19, 2016;
Accepted: Sep. 14, 2016;
Published: Dec. 17, 2016
Views 2815 Downloads 86
Amechi Sampson Nwankwegu, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
Chima Victor Nzomiwu, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
Nwanneka Chinazor Nwozor, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
Herbert Chijioke Eneite, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University PMB, Awka, Nigeria
In the present study, the prevalence of pathogenic bacteria from poultry environment was investigated. Potentially pathogenic organisms were isolated and identified notably; Salmonella enteritidis, Shigella sp., Clostridium perfringens, Pseudomonas sp., E. coli, Staphylococcus aureus, Vibrio cholerae and Vibrio parahemolyticus. The susceptibility of the isolates to different antibiotics was tested and it was observed that Pseudomonas sp. was very sensitive to the antibiotics, Ceporex (10µg) and Tarivd (10µg) with 16mm zone of inhibition. Clostridium sp. was more susceptible to Levofloxacin (20µg) with 20 mm zone of inhibition, Staph. aureus was more susceptible to Streptomycin (30µg) and Levoflaxacin (20µg) with 18mm zone of inhibition, E. coli was more susceptible to Gentamycin (10µg) with 20mm zone of inhibition, Salmonella enteritidis was more sensitive to Augmentin (30µg) with 20mm zone of inhibition and Shigella sp. was more sensitive to Ciproflox (10µg) with 20mm zone of inhibition. The pathogenicity of these isolates was studied by infecting each on mice. There was death of two mice infected with Clostridium perfringens. Mice infected with Shigella sp., suffered swollen of the scrotum and scrotal sac which was observed after dissection. Mice infected with Pseudomonas sp., Staphylococcus aureus, and Salmonella enteritidis and E. coli, 25x108, 8x108, 20x108, 10x108 cfu/ml of the infected organisms were recovered from their intestine respectively. It seems that the organisms colonized their intestine at high level and they shed them in their faeces, though the infections were asymptomatic at the stage.
Amechi Sampson Nwankwegu,
Chima Victor Nzomiwu,
Nwanneka Chinazor Nwozor,
Herbert Chijioke Eneite,
Evaluation and Antimicrobial Susceptibility Pattern of Pathogenic Bacteria in Poultry Wastes, International Journal of Microbiology and Biotechnology.
Vol. 1, No. 1,
2016, pp. 10-15.
Abakpa, G. O., Umoh, V. J., Ameh, J. B., Yakubu, S. E., Kwaga, J. K. P., Kamaruzaman, S. Diversity and antimicrobial resistance of Salmonella enterica isolated from fresh produce and environmental samples. Environmental Nanotechnology, Monitoring & Management. 2015. 3, 38–46.
Parker, C. T., Huynh, S., Quinones, B., Harris, L. J., Mandrell, R. E. Comparison of Genotypes of Salmonella enterica serova Entritidia phage type 30 and 9c strains isolated during three out breaks Associated with raw Almonds. Applied Environmental Microbiology. 2010. 76, 3723–3731.
Watson, C. H., and Edmunds, W. J. A review of typhoid fever transmission dynamic models and economic evaluations of vaccination. Vaccine. 2015. 33, 42–54.
Agblevor, F. A., Beis. S., Kim, S. S., Tarrant, R., Mante, N. O. Biocrude oils from the fast pyrolysis of poultry litter and hardwood. Waste Management and Research. 2010. 12, 2165–2172.
Adeoye, G. O., Sridhar, M. K. C, Mohammed, O. E. Poultry waste management for crop production: Nigerian experience. Waste Management and Research. 1994. 12, 2165–2172.
Kelleher, B. P., Leahy, J. J., Henihan, A. M, Dwyer, T. F., Sutton, D., Leahy, M. J. Advances in poultry litter disposal technology–a review. Elsevier. 2002. 83, 27–36.
Nwankwegu, A. S., Onwosi, C. O., Orji, M. U., Anaukwu, C. G., Okafor, U. C., Azi, F., Marins, P. E. Reclamation of DPK hydrocarbon polluted agricultural soil using a selected bulking agent. Journal of Environmental Management. 2016a. 172, 136–142.
Raghavendra, P., Halami, P. M. Screening, selection and characterization of phytic acid degrading lactic acid bacteria from chicken intestine. International Journal of Food Microbiology. 2009. 133, 129–134.
Resende, J. A., Silva, V. L., Rocha de Oliveira, T. L., de Oliveira Furtunato, S., de Casta Carneiro, J., Otenlo, M. H., Dinz, C. G. Prevalence and persistence of potentially pathogenic and antibiotic resistant bacteria during anaerobic digestion treatment of cattle manure. Bioresource Technology. 2014. 153, 284–291.
Ghaderpour, A., MohdNasori, K. N., Chew, L. L., Chong, V. C., Thong, K. L., Chaj, L. C. Detection of multiple potentially pathogenic bacteria in Matang mangrove estuaries, Malaysia. Marine Pollution Bulletin. 2014. 83, 324–330.
Alfa, M. I., Adje, D. B., Igboro, S. B., Oranusi, U. S., Dahunsi, S. O., Akali, D. M. Assessment of biofertilizer quality and health implications of anaerobic digestion effluent of cow dung and chicken droppings. Renewable Energy. 2014. 63. 681–686.
Agwu, E., Ihonobe, J. C., Ezeonwumelu, J. O., Loghi, M. M. Baseline burden and antimicrobial susceptibility of pathogenic bacteria recovered from oral lesions of patients with HIV/AIDS in South-WesternUganda. Oral Science International. 2015. 12, 59–66.
Loiki, M. R., dePaula, C. M. D., Langone, A. C. J., Rodrigues, R. Q., Cibulski, S., Rodrigues, R. O., Carmago, A. C., Nero, L. A., Mayer, F. Q., Tondo, E. C. Genotypic and antimicrobial characterization of pathogenic bacteria at different stages of cattle slaughtering in southern Brazil. Meat Science. 2016. 116,193–200.
Citterico, L., Franzvk, H., Palarasah, Y., Andersen, T. E., mateiu, R. V., Gram, L. Improved in vitro evaluation of novel antimicrobials: potential synergy between human plasma and antibacterial peptidomimetics, AMPs and antibiotics against human pathogenic bacteria. Research in Microbiology. 2016. 167, 72–82.
Assohoun-Dieni, N. M. C., Dieni, N. T., Messaoudu, S., Lhomme, E., Koussemon-Camara, M., Ouassa, T., Chobert, J. M., Onno, B., Dousset, X. Biodiversity, dynamics and antimicrobial activity of lactic acid bacteria involved in the fermentation of maize flour for dokluproduction in Côte d'Ivoire. Food Control. 2016. 62, 397–404.
Zwijnenburg, P. J. Experimental Pneumococcal Meningitis in Mice: Model of Intranasal Infection. The Journal of Infectious Disease. 2001. 183, 1143–1146.
Nwankwegu, A. S., Ikpe, E. M, Chukwura, E. I., Irondi, C. R., Obika, E. I. Ex Situ biodegradation of crude oil using bacterial isolates from palm oil mill effluent. American Journal of Life Sciences. 2016b. 4, 71–75.
Swai, E. S., and Schooman, L. A survey of Zoonotic Disease in trade cattle slaughtered at Tangia City abattoir: a cause of Public Health Concern. Asian Pacific Journal of Tropical Biomedicine. 2012. 2, 55–60.
Christian, S. H., Christa, M., Katrrin, S. M. Sabin, M., Stefanie, S., Kurin, S. Johann, B. Heavy metals in liquid pig manure in light of bacterial antimicrobial resistance. Environmental research. 2012. 113, 21–27.
Bollen, S. Presence of Wild-Type and Attenuated Salmonella enterica strains in Brain Tissues following Inoculation of Mice by Different Routes. The Journal of Infection and Immunity. 2008. 76, 3268–3272.
Fernandez- Miyakawa, E. M. Development and Application of an Oral Challenge Mouse Model for Clostridium perfringes type D. The Journal of Infection and Immunity. 2007. 75, 4282–4288.
Kondo, I., Kurosaka, K., Kimura, K., Masuda, S., Hasegawa, N. Studies on the mechanism of Staphylococcal infection by means of various mutants originated from Staphylococcus aureus no. 248.1. Comparison of the virulence of 248 α H and 248βH upon the intravenous infection of mice. Jikeikai Medical Journal. 1971. 86, 285–291.