Onion, Orange and Prickly Pear Peel Extracts Mixed with Beef Meatballs Ameliorate the Effect of Alloxan Induced Diabetic Rats
American Journal of Pharmacology and Phytotherapy
Volume 1, Issue 1, December 2016, Pages: 15-24
Received: Oct. 17, 2016; Accepted: Nov. 14, 2016; Published: Dec. 12, 2016
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Yousif Elhassaneen, Nutrition and Food Science Department, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt
Samia Goarge, Division of Home Economics, Faculty of Specific Education, Port Said University, Port Saied, Egypt
Ryeaan Sayed, Division of Home Economics, Faculty of Specific Education, Port Said University, Port Saied, Egypt
Mayada Younis, Division of Home Economics, Faculty of Specific Education, Port Said University, Port Saied, Egypt
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The present study was carried out to investigate the effectiveness of three plant parts methanol extracts (orange peel extract, OPE, red onion skin powder, ROSE, and prickly pear extract, PPE) mixed with beef meatballs in modulating hyperglycemia using alloxan induced diabetic rats model. Treatment of animals with alloxan caused a significant increase (p≤0.05) in serum glucose concentration (49.47%) compared to normal control group. Compared to the diabetic control, hypoglycemic capability of beef meatballs supplemented with 0.1% plant parts methanolic extracts was demonstrated by significant (p≤0.05) decreasing of glucose concentration in serum. The rate of decreasing was recorded 29.91, 39.99, 31.15 and 41.75% for meatballs supplemented with OPE, ROSE, PPPE and their mixture, respectively. The same behavior was recorded for liver tissue malonaldialdehyde (MDA) level, the biomarker of oxidative stress in liver. Activities of AST, ALT and ALP were increased significantly (P<0.05) in alloxan-induced diabetic rats in comparison with normal control group. Treatment with meatballs mixed with selected methanolic extracts to diabetic rats significantly (P < 0.05) decreased the elevated AST, ALT and ALP almost near to normal levels. In conclusion, OPE, ROSE and PPPE have ameliorated the effect of alloxan induced diabetes in rats. So, we advise to use these plant parts extracts in human nutrition as natural food additives for their anti-hyperglycemic effects.
Plant Parts, Liver Functions, Aminotransferases, Glucose, Malonaldialdehyde, Glutathione
To cite this article
Yousif Elhassaneen, Samia Goarge, Ryeaan Sayed, Mayada Younis, Onion, Orange and Prickly Pear Peel Extracts Mixed with Beef Meatballs Ameliorate the Effect of Alloxan Induced Diabetic Rats, American Journal of Pharmacology and Phytotherapy. Vol. 1, No. 1, 2016, pp. 15-24. doi: 10.11648/j.ajpp.20160101.14
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WHO, (2006). Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia: report of a WHO/IDF consultation. Geneva, Switzerland.
Wild, S.; Roglic, G., Green, A.; Sicree, R. and King H. (2004): Global Prevalence of Diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care. 27: 1047-1053.
Stumvoll, M.; Goldstein, B. J. and van Haeften, T. W. (2005): Type 2 diabetes: principles of pathogenesis and therapy. Lancet, 365:1333-1346.
WHO, (1999). Definition, Diagnosis and Classification of Diabetes Mellitus and its Complications: Report of a WHO Consultation. Part 1: Diagnosis and Classification of Diabetes Mellitus. Geneva.
Levitan, E. B.; Song, Y.; Ford, E. S. and Liu, S. (2005): Is nondiabetic hyperglycemia a risk factor for cardiovascular disease? A meta-analysis of prospective studies. JAMA, 293: 194-202.
Matsui, T.; Ogunwande, L. A.; Abesundara, K. J. and Matsumoto K. (2006): Antihyperglycemic potential of natural products. Mini-Rev Med Chem, 6:109-120.
Dias, A.; Porawski, M.; Alonso, M.; Marroni, N.; Collado, P.S. and Gonzalez-Gallego, J. (2005): Quercetin decreases oxidative stress, NF-kappaB activation, and iNOS over expression in liver of streptozotocin-induced diabetic rats. J Nutr,135:2299-2304.
Coskun O., Kanter M., Korkmaz A. and Oter S. (2005): Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas. Pharmacol Res, 51:11.
Jung. J.;Yeni, L.; Min, S.; JiYeon, K. and Oran, K.(2011): Onion peel extracts ameliorate hyperglycemia and insulin resistance in high fat diet/streptozotocin-induced diabetic rats, Nutrition & Metabolism, 8:18:2-8.
Kobori, M.; Masumoto, S.; Akimoto, Y. and Takahashi Y.(2009): Dietary quercetin alleviates diabetic symptoms and reduces streptozotocin-induced disturbance of hepatic gene expression in mice. MolNutr Food Res, 53: 859-868.
Aguirre, L.; Maria, P.; Elizabeth, H. and Luis, B. (2014): Effects of resveratrol and other polyphenols in hepatic steatosis. World J Gastroenterol. 21; 20(23): 7366-7380.
Hertog, M. G.; Hollman, P. C. and Venema, D. P. (1992): Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits. J Agric Food Chem, 40:1591-1598.
Waldron, K. (2001): Useful ingredients from onion waste. Food Science and Technology, 15, 38–39.
Price, K. R.. and Rhodes, M. J. C. (1997): Analysis of the major flavonol glycosides present in four varieties of onion (Allium cepa) and changes in composition resulting from autolysis. Journal of the Science of Food and Agriculture, 74, 331–339.
Hollman, P.; Van Trijp, J.; Buysman, M.; Gaag, M.; Mengelers, M.; deVries, J. and Katan, M. (1997): Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. FEBS Letters. 418: 152-156.
Kanter, M.; Altan M. F.; Donmez S.; Ocakci A. and Kartal M. E. (2007): The effects of quercetin on bone minerals, biomechanical behavior, and structure in streptozotocin-induced diabetic rats. Cell Biochem Funct, 25: 747-752.
Nemeth, K. and Piskula, M. (2007): Food content, processing, absorption and metabolism of onion flavonoids. Crit Rev Food Sci Nutr, 47:397-409.
Abd El-Razek, F. H. and Hassan, A. H. (2011): Nutritional Value and Hypoglycemic Effect of Prickly Cactus Pear (OpuntiaFicus-Indica) Fruit Juice in Alloxan-Induced Diabetic Rats. Australian Journal of Basic and Applied Sciences, 5(10): 356-377.
Mashal, R. (2016). Technological and chemical studies on the fortification of bakery products with phytochemicals. Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt.
Elhassaneen, Y.; Ragab, S. and and Saleh A. (2016). Effect of Selected Plant Parts Extracts on Liver Injuries Induced by CCl4 in vitro. Pyrex Journal of Medicinal Plant Research, 2 (2): 8-20.
Cardador-Martínez, A.; Jiménez-Martínez, C. and Sandoval, G. (2011): Revalorization of cactus pear (Opuntia spp.) wastes as a source of antioxidants. Ciê e Tecn. Alim, 31: 782-788.
Abou-Elella, F. and Ali, R. (2014): Antioxidant and Anticancer Activities of Different Constituents Extracted from Egyptian Prickly Pear Cactus (OpuntiaFicus-Indica) Peel. Biochem Anal Biochem, 3: 158.
Askar, A. and Treptow, H. (1998): Nebenproduktebei der Verarbeitungtropischer Fruchte. Industrielle Obst- und Gemüseverwertung; 83, 7–13.
Amin, I.; Zamaliah, M. and Chin, W. (2004): Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry; 87(4): 581-586.
Badawy, R. (2016). The effect of phytochemical extracts of some plant parts in liver cancer initiation induced by benzo(a)pyrene. Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Egypt.
Saleh, A. (2016). Study the effect of nutritional antioxidant on liver injuries induced by carbon tetrachloride in vitro". Ph.D. Thesis in Nutrition and Food Science, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt.
AIN, American Institute of Nutrition. (1993): Purified diet for laboratory Rodent, Final report. J. Nutrition. 123:1939-1951.
Campbell, J. A. (1963): Methodology of Protein Evaluation.RGA Nutr. Document R. Led. 37. June meeting, New York.
Hegested, D.; Mills, R. and Perkins, E. (1941): Salt mixture. J. Biol. Chem, 138:459.
NRC, National Research Council (1996): Guide for the Care and Use of Laboratory Animals Washington: National Academy Press.
Lazarow, A. and Palay, B. (1954):"Experimental Diabetes and its relation to the Disease" A symposium. Black wells scientific Publication, 14: 66 – 69.
Wohaieb, S. A. and Godin, D. V. (1987): Alterations in free radical tissue defense mechanisms in streptozotocin induced diabetes in rat. Effects of insulin treatment. Diabetes, 36:1014 – 18.
Kakkar, R.; Mantha, S. V.; Radhi, J. and Prasad, K. (1998): Increased oxidative stress in rat liver and pancreas during progression of streptozotocin – induce diabetes. Clinical Science, 94: 623-32.
Stroev, E. A. and Makarova, V. G. (1989): Laboratory Manual in Biochemistry, MIR Publishers, Moscow, USSR.
Yound, D. S. (1975): Determination of GOT. Clin. Chem., 22(5):1-21.
Tietz, N. W. (1976): Fundamental of Clinical Chemistry. Philadelphia, W.B. Saunders, P. 243.
Moss, D. W. (1992): Alkaline phosphatase isoenzymes. Clin. Chem., 28: 2007-2016.
Dohnal L.; Kalousová M. and Zima T. (2010). Comparison of three methods for determination of glucose. Prague Medical Report / 111 (1): 42–54.
Tietze, F. (1969): Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem. 27: 502-522.
Sheela, C.; Kumud, K., and Augusti, K. (1995). Anti-diabetic effects of onion and garlic sulfoxide amino acids in rats. Planta Med. 61: 356-357.
Suresh Babu, P. and Srinivasan, K. 1997. Influence of dietary capaicin and onion an the metabolic abnormalities associated with streptozotocin induced diabetes mellitus. Molecular and Cellular Biochemistry, 175: 49-57.
Mohammadian, M.; Zeinab M. and Reza, H. (2011): Bioactive compounds and antioxidant capacities in the flavedo tissue of two citrus cultivars under low temperature. Braz. J. Plant Physiol., 23(3): 203-208.
Gil, M. I.; Tomas-Barberan, F. A.; Hess-Pierce, B.; Holcroft, D. M., and Kader, A. A. (2000): Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48, 4581–4589.
Noda, Y.; Kaneyuki, T.; Mori, A. and Packer, L. (2002): Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin. Journal of Agricultural and Food Chemistry, 50, 166–171.
Pagana, K. D. and Pagana, T. J. (1997): Mosby's diagnostic and laboratory test references. 3 rd ed., Mosby-year Book, Inc., New York.
Harris, E. H. (2005): Elevated liver function tests in type 2 diabetes. Clinical Diabetes, 23 (3): 115-119.
Onyeneho, S. N. and Hettiarachchy, N. S. (1993): Antioxidant activity, fatty acids and phenolic acids composition of potato peels. Journal of the Science of Food and Agriculture, 62, 345–350.
Beattic, J.; Crozier, A. and Duthie, G. (2005): Potential Health Benefits of berries. Current Nutrition & Food Science. 1: 71-86.
El-Nashar, N. G. (2007): Development of primary liver cell culture from fish as a valuable tool in nutrition and biotechnology research. Ph.D. Thesis, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt.
Elhassaneen, Y.; Ragab, S.; El-Beltagi, A. and Emad, A. (2013): Mango peel powder: A potential source of phenolics, carotenoids and dietary fiber in Biscuits preparations 2nd International-16th Arab Conference of Home Economics "Home Economics in the Service of Industry"10-11 September, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt.
Dawson, R. M. (1998): The toxicology of microcystins. Toxicon., 36: 953- 962.
Sayed- Ahmed, S. (2016): Nutritional and technological studies on the effect of phytochemicals on obesity injuries and their related diseases by using experimental animals Ph.D. Thesis in Home Economics (Nutrition and Food Science), Faculty of Specific Education, Port Said University, Egypt.
Tiwari, A. K. and Madhusudana, J. R. (2002): Diabetes mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects. Current Science, 83 (1): 30-38.
Cameron, N. E. and Cotter, M. A. (1994) The Relationship of Vascular Changes to Metabolic Factors in Diabetes Mellitus and Their Role in the Development of Peripheral Nerve Complications. Diabetes/Metabolism Reviews, 10, 189-224.
Hotamisligil, G. S. (2006). Inflammation and metabolic disorders. Nature, 444:860-867.
Defronzo, R. A. and Tripathy, D. (2009). Skeletal muscle insulin resistance is the primary defect in type 2 diabetes. Diabetes Care, 32:S157-S163.
Nguyen, M. T.; Favelyukis, S.; Nguyen, A. K.; Reichart, D.; Scott, P. A.; Jenn, A.; Liu- Bryan, R.; Glass, C. K.; Neels J. G. and Olefsky, J. M. (2007): A subpopulation of macrophages infiltrates hypertrophic adipose tissue and is activated by free fatty acids via Toll-like receptors 2 and 4 and JNK-dependent pathways. J Biol Chem, 282:35279-35292.
Cieslik E.; Greda A. And Adamus, W. (2006): Contents of polyphenols in fruit and vegetables. Food Chem 94: 135-142.
El-safty, S. M. and Al-Masri, S. A. (2009): Effect of prickly pear consumption on some biological and biochemical changes in hyperglycemic injured rats. Bulletin of the National Nutrition Institute, 34: 46-61.
Akacha, A.; Tarek, R., Lazhar Z. and Mohamed A. (2015): Preventive effect of ethanolic extract of cactus (Opuntia ficus-indica) cladodes on methotrexate induced oxidative damage of the small intestine in wistar rats. Archives of Medicine, 7 (6): 1-7.
Motawe, E. H.; Ebeid, Hanan, H. M.; AL-Sayed, M. A. and Elbehairy, S. A. (2012): Biological Evaluation of Natural Antioxidants from Residual Sources. World Journal of Dairy & Food Sciences 7 (2): 174-180.
Tesoriere, L.; Butera, D.; Pintaudi, A. M.; Allegra, M and Livrea, M. A. (2004): Supplementation with cactus pear (Opuntia ficus-indica) fruit decreases oxidative stress in healthy humans: a comparative study with vitamin C. Am J Clin Nutri 80: 391-395.
Oboh, G. and Ademosun, A. (2012): Characterization of the antioxidant properties of phenolics extracts from some citrus peels. J Food Sci Technol., 49(6):729–736.
Lu, S. C. (1999): Regulation of hepatic glutathione synthesis: current concepts and controversies, FASEB J., 1999, 13, 1169–1183.
Gregus, Z.; Fekete, T.; Halaszi, E. and Klaassen, C. D. (1996): Lipoic acid impairs glycine conjugation of benzoic acid and renal excretion of benzoylglycine, Drug Metab. Dispos., 24: 682–688.
Jain, S. K. and McVie, R. (1994): Effect of glycemic control, race (white versus black) and duration of diabetes on reduced glutathione content in erythrocytes of diabetic patients, Metabolism, 43: 306–309.
Ceriello, A. (2000): Oxidative stress and glycemic regulation, Metabolism, 49: 27–29.
Seghrouchni, I.; Jocelyne, D.; Edith B.; Rivière, P.; Calmard, J. and Orgiazzi, A. (2002): Oxidative stress parameters in type I, type II and insulin-treated type 2 diabetes mellitus; insulin treatment efficiency, Clinica Chimica Acta, 321: 89–96.
Moussa, S. A. (2008): Oxidative stress in diabetes mellitus. Romanian J. Biophys., 18 (3): 225-236.
Paglia, D. E. and Valentine, W. N. (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase, J. Lab. Clin. Med., 70: 158-69.
Lee, A. Y. and Chung S. S. (1999): Contributions of polyol pathway to oxidative stress in diabetic cataract, FASEB J., 13: 23-30.
Fayez, S. (2016): The effect of turmeric and curcumin on liver cancer induced by benzo[a]pyrene in rats. M.Sc. Thesis in Home Economics, Faculty of Specific Education, Port Said University, Port Said, Egypt.
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