Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect
American Journal of Chemical and Biochemical Engineering
Volume 1, Issue 1, December 2017, Pages: 7-16
Received: Sep. 8, 2016;
Accepted: Dec. 7, 2016;
Published: Jan. 7, 2017
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Li Huiduan, Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, P. R. China
Yu Jianzhong, Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, P. R. China
Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.
Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect, American Journal of Chemical and Biochemical Engineering.
Vol. 1, No. 1,
2017, pp. 7-16.
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