Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis
Volume 3, Issue 6, November 2017, Pages: 114-118
Received: Oct. 19, 2017;
Accepted: Nov. 1, 2017;
Published: Dec. 5, 2017
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Rongkai Zhang, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Guowei Li, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Dong Jiang, Department of Urology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Lukun Yang, Department of Anesthesiology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Dawei Zhang, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Zhe Wang, Department of Orthopaedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
Jun Liang, Department of Stomatology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China
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Osteoarthritis (OA) is characterized by the degeneration of affected joints leading to pain and reduced mobility. Periostin appears as a potential candidate in transducing mechanical signals to stimulate chondrocyte proliferation. 15 rats (E-group) underwent both medial meniscectomy and medial collateral ligament (MCL) transaction, while the other 15 rats (S-group) underwent sham surgery. Animals were killed at 1, 2, and 4 weeks postsurgery, and 5 animals were put into use per-time point in each treatment group. Cartilage was harvested from tibial plateau and femoral condyle. We examined the level of Postn expression in cartilage of the experimental OA, using real-time PCR and immunohistochemistry analysis. The expression of Postn was about 2-fold higher at 1 week (P=0.08<0.05), 4-fold higher at 2 weeks (P=0.003<0.05) and 4-fold higher at 4 weeks (P=0.034<0.05) post-surgery in E-group compared to S-group. Postn protein expression levels were markedly increased in the E-Group compared with the S-Group at 1, 2 and 4 weeks post-surgery. This study will be of benefit to those subsequent studies using the OA model to evaluate the outcomes of Postn epxpression in mechanism of OA. Postn may play prominent roles in pathogenic mechanisms of OA.
Osteoarthritis (OA), Periostin (Postn), Cartilage, Rat Model of Osteoarthritis
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Elevated Expression of Periostin in Cartilage in Early Experimental Osteoarthritis, Biomedical Sciences.
Vol. 3, No. 6,
2017, pp. 114-118.
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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