International Journal of Science and Qualitative Analysis
Volume 1, Issue 1, May 2015, Pages: 6-10
Received: May 22, 2015;
Accepted: May 27, 2015;
Published: May 28, 2015
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Alma Daja, Clinical and Biochemical Laboratory, Health Centre nr 2, Tirana, Albania
Entela Treska, University Hospital of Obstetrics and Gynaecology “Queen Geraldine”, Tirana, Albania
The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.
The Impact of Enzymes in the Hepatic Function, International Journal of Science and Qualitative Analysis.
Vol. 1, No. 1,
2015, pp. 6-10.
Bennett, T. P., and Frieden, E.: Modern Topics in Biochemistry, pg. 43-45, Macmillan, London (1969).
Holum, J.: Elements of General and Biological Chemistry, 2nd ed., 377, Wiley, NY (1968).
Martinek, R.: Practical Clinical Enzymology: J. Am. Med. Tech., 31, 162 (1969).
Harrow, B., and Mazur, A.: Textbook of Biochemistry, 109, Saunders, Philadelphia (1958).
Pfeiffer, J.: Enzymes, the Physics and Chemistry of Life, pg 171-173, Simon and Schuster, NY (1954)
Tietz, N. W. Ed., Fundamentals of Clinical Chemistry (W. B. Saunders Co., Philadelphia, Pa., 1970).
Stryer L, Berg JM, Tymoczko JL (2002).Biochemistry (5th ed.). San Francisco: W.H. Freeman. ISBN 0-7167-4955-6.
Schomburg I, Chang A, Placzek S, Söhngen C, Rother M, Lang M et al. (Jan 2013). "BRENDA in 2013: integrated reactions, kinetic data, enzyme function data, improved disease classification: new options and contents in BRENDA". Nucleic Acids Research 41 (Database issue): D764–72. doi: 10.1093/ nar/gks 1049. PMID 23203881.
Radzicka A, Wolfenden R (Jan 1995). "A proficient enzyme". Science 267 (5194): 90–931. doi:10.1126/science.7809611. PMID 7809611.
Callahan BP, Miller BG (Dec 2007). "OMP decarboxylase--An enigma persists".Bioorganic Chemistry 35 (6): 465–9. doi: 10.1016/j.bioorg. 2007.07.004 PMID 17889251.
Horton, R. Moran, L.A. Scrimgeour, G. Perry, M. Rawn, D. (2005) Principles of Biochemistry 4th Edition, Pearson Education (US)
Becker, W. Kleinsmith, L.J. Hardin, J. Bertoni, G.P. (2008) The World of the Cell, Benjamin Cummings
Anon. , Clini cal Me thods Manual, Bausch & Lomb , Inc., Rochester, N.Y. (1965)
Johnston DE (1999). "Special considerations in interpreting liver function tests". Am Fam Physician 59 (8): 2223–30. PMID 10221307.
McClatchey, Kenneth D. (2002). Clinical laboratory medicine. Lippincott Williams & Wilkins. pp. 288–. ISBN 978-0-683-30751-1. Retrieved 5 August 2011.
Mengel, Mark B.; Schwiebert, L. Peter (2005). Family medicine: ambulatory care & prevention. McGraw-Hill Professional. pp. 268–. ISBN 978-0-07-142322-9. Retrieved5 August 2011.
Kennedy I.W. “User evaluation of precision performance of clinical chemistry devices”, NCCLS, Vol 4, No 8, page 185-195, 1989
Wachtel M. “Creation and verification of reference intervals laboratory medicine”, No 26, page 593-597, 1995.