Cholestasis Detection Enzyme Tests, in Pregnant Women with Different Hospitalization Diagnosis
International Journal of Science and Qualitative Analysis
Volume 1, Issue 1, May 2015, Pages: 1-5
Received: May 11, 2015;
Accepted: May 18, 2015;
Published: May 22, 2015
Views 3989 Downloads 147
Entela Treska, University Hospital of Obstetrics and Gynaecology “Queen Geraldine”, Tirana, Albania
Alma Koci, Clinical and Biochemical Laboratory, Health Centre nr 2, Tirana, Albania
The aim of the study is the evaluation of serum Alkaline Phosphatase and Gamma-glutamyl Transferase levels in pregnant women as indicators of cholestasis. Cholestasis is defined as a decrease in bile flow due to the obstruction of it through intra/extra hepatic bile ducts. GGT enzyme was previously thought to be helpful in confirming a hepatic source of ALP however, GGT elevations lack the necessary specificity to be a useful confirmatory test for ALP. We analyzed 500 cases of pregnant women from year 2011-2013. Serum enzyme levels (ALP and GGT) were measured respectively using Beckman Synchron LX 20 and Enzymatic method Activity Colorimetric Kit, at the Medical Laboratory “PhD. Stelijan Buzo” in Tirana. According to the laboratory reference values of ALP (100-290 U/L): 50% of the cases resulted normally pregnant women, 50% suffering from liver disease (<100 U/L or >290 U/L); whereas according to values of GGT (8-31 U/L): 75% resulted normally pregnant women, 25% with liver damage (<8 U/L or >31 U/L). Micronutrients deficiency leads to irreversible disorders of development; Nutritional advice would have been more effective either for the mother and the future of her child. There are few reported cases of young mothers, with sufficient income, which limit some foods due to fear of increasing body weight, while forgetting that the lack of appropriate micro-nutrients, directly affects the health of growing babies.
Cholestasis Detection Enzyme Tests, in Pregnant Women with Different Hospitalization Diagnosis, International Journal of Science and Qualitative Analysis.
Vol. 1, No. 1,
2015, pp. 1-5.
PDB 1ALK: Kim EE, Wyckoff HW "Reaction mechanism of alkaline phosphatase based on crystal structures. Two-metal ion catalysis". J. Mol. Biol. 218 (2): 449–64. doi:10.1016/0022-2836(91)90724-K. PMID 2010919, (March 1991).
Tamás L, Huttová J, Mistrk I, Kogan G "Eﬀect of Carboxymethyl Chitin-Glucan on the Activity of Some Hydrolytic Enzymes in Maize Plants" (PDF).Chem. Pap. 56 (5): 326–329 (2002).
Tamás L, Huttová J, Mistrk I, Kogan G "Effect of Carboxymethyl Chitin-Glucan on the Activity of Some Hydrolytic Enzymes in Maize Plants". Chem. Pap. 56 (5): 326–329, (2002).
Chernecky CC, Berger BJ Laboratory Tests and Diagnostic Procedures, 5th ed. St. Louis: Saunders (2008).
Fischbach FT, Dunning MB III, eds. Manual of Laboratory and Diagnostic Tests, 8th ed. Philadelphia: Lippincott Williams and Wilkins (2009).
Beckman Synchron LX Systems Chemistry Information Manual, 2001.
Tietz, N.W. Textbook of Clinical Chemistry, W.B. Saunders, Philadelphia, PA (1986).
Tietz, N.W., “Specimen Collection and Processing; Sources of Biological Variation,” Textbook of Clinical Chemistry, 2nd Edition, W.B. Saunders, Philadelphia, PA (1994).
National Committee for Clinical Laboratory Standards, Procedures for the Handling and Processing of Blood Specimens, Approved Guideline, NCCLS publication H18-A, Villanova, PA (1990).
Tietz, N.W., ed., Clinical Guide to Laboratory Tests, 3rd Edition, W.B. Saunders, Philadelphia, PA (1995).
National Committee for Clinical Laboratory Standards, How to Define, Determine, and Utilize Reference Intervals in the Clinical Laboratory, Approved Guideline, NCCLS publication C28-A, Villanova, PA (1995).
Tietz, N.W., ed., Fundamentals of Clinical Chemistry, 3rd Edition, W.B. Saunders, Philadelphia, PA (1987).
Henry, J.B., ed., Clinical Diagnosis and Management by Laboratory Methods, 18th Edition, W.B. Saunders, Philadelphia, PA (1991).
Young, D.S., Effects of Drugs on Clinical Laboratory Tests, 4th Edition, AACC Press, Washington, D.C. (1995).
Friedman, R.B. and D.S. Young, Effects of Disease on Clinical Laboratory Tests, 3rd Edition, AACC Press, Washington, D.C. (1997).
Young, D.S., Effects of Preanalytical Variables on Clinical Laboratory Tests, 2nd Edition, AACC Press, Washington, D.C. (1997).
National Committee for Clinical Laboratory Standards, Method Comparison and Bias Estimation Using Patient Samples; Approved Guideline, NCCLS publication EP9-A, Villanova, PA (1995).
National Committee for Clinical Laboratory Standards, Precision Performance of Clinical Chemistry Devices, Tentative Guideline, 2nd Edition, NCCLS publication EP5-T2, Villanova, PA (1992).
Tate SS, Meister A "gamma-Glutamyl transpeptidase from kidney".Methods in Enzymology 113: 400–419. doi:10.1016/S0076-6879(85)13053-3.ISBN 978-0-12-182013-8. PMID 2868390, (1985).
Courtay C, Oster T, Michelet F, Visvikis A, Diederich M, Wellman M et al. "Gamma-glutamyltransferase: nucleotide sequence of the human pancreatic cDNA. Evidence for a ubiquitous gamma-glutamyltransferase polypeptide in human tissues". Biochemical Pharmacology 43 (12): 2527–2533. doi:10.1016/0006-2952(92)90140-E. PMID 1378736, (Jun 1992).
Vázquez-Medina, J. P., et al. (April 2011) Prolonged fasting increases glutathione biosynthesis in post weaned northern elephant seals. J. Exp. Biol., 214: 1294 – 1299
Tate SS, Meister A "gamma-Glutamyl transpeptidase from kidney". Meth. Enzymol. Methods in Enzymology 113: 400–419. ISBN 978-0-12-182013-8, (1985).