Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon
European Journal of Clinical and Biomedical Sciences
Volume 5, Issue 3, June 2019, Pages: 46-50
Received: May 24, 2019; Accepted: Jul. 1, 2019; Published: Jul. 24, 2019
Views 690      Downloads 99
Jean Teghonong, Department of Biological Sciences, Distant Production House University, Uvira, Democratic Republic of Congo; Diagmed Laboratory, Douala, Cameroon
Cecile Okalla Ebongue, Department of Biological Sciences, Douala University, Douala, Cameroon
Callixte Yadufashije, INES-Ruhengeri - Institute of Applied Sciences, Musanze, Rwanda
Dieudonne Adiogo, Department of Biological Sciences, Douala University, Douala, Cameroon
Article Tools
Follow on us
Miniaturized galleries are essential for a good identification of bacteria. The aim of this study was to identify multi-resistant Klebsiellap neumoniae strains in the referral health structures of Cameroon littoral region and establish a digital profile. A cross-sectional and descriptivestudy was conducted during 2016 and early 2017, intermediate susceptibility isolates or resistant to cephalosporins third generation (C3G) were collected in the laboratories of referral hospitals of the Littoral (General Hospital, Laquintinie, Military, Gyneco-obstetrics and pediatrics of Douala and Saint John of Malta of Njombé) and in Diagmed laboratory. The confirmation of the strains was made by the gallery API 20E™ (BioMérieux). In this study, atotal of 412 strains of Klebsiellasp (species) were isolated. We obtained 125 strains of Klebsiellasp intermediate or resistant to (C3G) and 122 were confirmed to be Klebsiella pneumoniae. The sex ratio (F / M) was 1.26. Klebsiella pneumoniae pneumonia was the species mostly identified at 82.4% and the two digital profiles mainly found were 5215773 and 1215773 with respective frequencies of 52.8% and 18.4%. The biochemical profile: LDC (+), CIT (+), URE (+), IND (-), VP (+), GLU (+), MAN (+), INO (+), SOR (+), RHA (+), SAC (+), MEL (+), ARA (+) was found in all services. There is a high frequency of Klebsiella pneumoniae pneumonia with several different digital profiles, so it is still essential to make a good identification to facilitate the epidemiological data.
Gallery, Identification, Klebsiella Pneumoniae, Digital Profile
To cite this article
Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Dieudonne Adiogo, Digital Profile of Klebsiella Pneumoniae Strains Producing Extended Spectrum β-Lactamase Isolated in Hospitals in the Littoral Region of Cameroon, European Journal of Clinical and Biomedical Sciences. Vol. 5, No. 3, 2019, pp. 46-50. doi: 10.11648/j.ejcbs.20190503.12
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Kumamoto Y, Tsukamoto T, Matsukawa M, Kunishima Y, Hirose T, Imafuku Y et al. Comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2004). I. Susceptibility distribution. Jpn J Antibiot. 2006 Jun; 59 (3): 177-200.
Bush K, Jacoby GA, Medeiros AA. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother. 1995; 39 (6): 1211-1233.
Guessennd N, Kacou-N’doubaA, GbononV, YapiD, EkazaE, Dosso M et al. Prévalence et profil de résistance des entérobactéries productrices de beta-lactamasesa spectre élargi (BLSE) à Abidjan Côte d’ivoire de 2005 à 2006. J. sci. pharm. biol. 2008; 9 (1): 63-70.
Philippon A, Arlet G, and Lagrange PH. Origin and impact of plasmid-mediated extendedspectrum beta-lactamases. Eur J ClinMicrobiolInfect Dis. 1994; 13 (1): S17-29.
Freney J, Leclerq R, Riegel P. Précis de Bactériologie clinique. 2è édition. Paris: Editions ESKA; 2007. p. 1012.
Chafa-BetbeuiA, Gonsu-Kamga H, Toukam M, Mbakop CD, Enjema-Lyonga E, Bilong S et al. Phenotypic Detection of Extended Spectrum Beta-Lactamase and Carbapenemases Produced by Klebsiellaspp Isolated from Three Referrals Hospitals in Yaounde, Cameroon. BrMicrobiologyRes J. 2015; 9 (1): 1-9.
Zineb-Belbel. Evaluation de la résistance aux antibiotiques des souches deKlebsiellapneumoniaeisolées dans les hôpitaux de la ville d’Annaba. Thèse de Doctorat en microbiologie. Université Badji Mokhtar Annaba; 2014, 66-67.
Sekhri-Arafa. Fréquence et marqueurs épidémiologiques de Klebsiellapneumoniaedans les services à haut risque infectieux au niveau du CHU Benbadis de Constantine. Thèse de Doctorat en Biochimie et Microbiologie. Université Mentouri de Constantine; 2011, 92-96.
Decré D, Gachot B, Lucet JC, Arlet G, Régnier B. Surveillance épidémiologique des souches de Klebsiellapneumoniae productrices de β-lactamases à spectre étendu (K. p BLSE) dans un service de réanimation. Rev Française des laboratories. 2000; 2000 (320): 31-38.
Okalla-Ebongue C, Mayoudom-Nguatcheussi C, Nda-Mefo JP, Bollanga J, Adiogo D, Namme-Luma H. Microbial Ecology and Antibiotic Susceptibility Profile of Germs Isolated from Hospital Surfaces and Medical Devices in a Reference Hospital in Douala (Cameroon). Scientific Research. 2018; 8 (2): 125-137.
Feizabadi MM, Mahamadi-Yeganeh S, Mirsalehian A, Mirafshar SM, Mahboobi M, Nili F, et al. Genetic characterization of ESBL producing strains of Klebsiella pneumoniae fromTehran hospitals. J Infect Dev Ctries. 2010; 4 (10): 609-615.
Dedeic-Ljubovic A, Hukic M, Pfeifer Y, Witte W, Padilla E, López-Ramis I, Albertí S. Emergence of CTX-M-15 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina. Clin Microbiol Infect. 2010; 16 (2): 152-6.
Elhani D, Bakir L, Mahjoub A. Changement de l’épidémiologie de Klebsiella pneumoniae productrice de β-lactamases à spectre élargi. J Pat Bio. 2011; 69 (5): 523-9.
Djuikoue IC, Woerther PL, Toukam M, Burdet C, Ruppé E, Gonsu KH et al. Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon. J Infect Dev Ctries. 2016 Oct 31; 10 (10): 1135-1139.
Hailaji NSM, Ould-Salem ML, Ghaber SM. La sensibilité aux antibiotiques des bactéries Uropathogènes dans la ville de Nouakchott-Mauritanie. J. Purol. 2016; 26 (6): 346-352.
Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital. European Journal of Clinical and Biomedical Sciences. 2017; 3 (6): 101-108.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186