The Importance of Myeloid-Derived Suppressor Cells in the Regulation of Immunosuppressive Mechanisms by Severe Fever with Thrombocytopenia Syndrome
European Journal of Clinical and Biomedical Sciences
Volume 4, Issue 5, October 2018, Pages: 63-68
Received: Nov. 15, 2018; Accepted: Dec. 13, 2018; Published: Jan. 7, 2019
Views 175      Downloads 22
Authors
Liping Sun, Department of Infectious Diseases, Miyun Teaching Hospital, Capital Medical University, Beijing, PR China; Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China
Huixia Zhao, Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China
Yajiao Li, Center for Stem Cell Research and Application, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China
Lizhen Sun, Department of Infectious Diseases, Miyun Teaching Hospital, Capital Medical University, Beijing, PR China
Huiyu Li, Center for Stem Cell Research and Application, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China
Shenghua Jie, Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China
Article Tools
Follow on us
Abstract
Objective: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus (SFTSV) with a high fatality rate. The objective of our study was to assess the mechanisms of immunofunction through detecting the presence of myeloid-derived suppressor cells (MDSC), granulocyte-colony stimulating factor (G-CFS) and T cells in SFTS patients. Methods: Serum samples from 30 SFTS cases and 20 healthy donors were collected for the test with flow cytometry and sandwich ELISA which contains CD3+, CD4+ T lymphocytes, subsets of MDSC and G-CSF. Results: Granulocytic MDSC (G-MDSC) and monocytic MDSC (M-MDSC) were significantly elevated in SFTS compared to normal control, and G-CSF was expressed at increased frequency. In contrast, CD3+ and CD4+ T lymphocytes were significantly diminished. Further analysis revealed that G-MDSC and G-CSF were higher in severe SFTS infection compared to the patients in mild SFTS infection, and the numbers of CD3+ and CD4+ T lymphocytes showed a more robust pattern of depression. Conclution: In summary, we have characterized an immunosuppressive mechanism in SFTSV infection dependent on G-CSF induction on MDSC and MDSC suppressing T cells.
Keywords
Severe Fever with Thrombocytopenia Syndrome (SFTS), Myeloid-derived Suppressor Cells (MDSC), Granulocyte-colony Stimulating Factor (G-CFS), T cells, Immunosuppression
To cite this article
Liping Sun, Huixia Zhao, Yajiao Li, Lizhen Sun, Huiyu Li, Shenghua Jie, The Importance of Myeloid-Derived Suppressor Cells in the Regulation of Immunosuppressive Mechanisms by Severe Fever with Thrombocytopenia Syndrome, European Journal of Clinical and Biomedical Sciences. Vol. 4, No. 5, 2018, pp. 63-68. doi: 10.11648/j.ejcbs.20180405.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Nagaraj S, Schrum AG, Cho HI, Celis E, Gabrilovich DI. Mechanism of T cell tolerance induced by myeloid-derived suppressor cells. J Immunol. 2010, 184: 3106-3116.
[2]
Zea AH, Rodriguez PC, Atkins MB, Hernandez C, Signoretti S, Zabaleta J, McDermott D, et al. Arginase-producing myeloid suppressor cells in renal cell carcinoma patients: a mechanism of tumor evasion. Cancer Res. 2005, 65: 3044-3048.
[3]
Youn JI, Gabrilovich DI. The biology of myeloid-derived suppressor cells: the blessing and the curse of morphological and functional heterogeneity. Eur J Immunol. 2010, 40: 2969-2975.
[4]
Gabrilovich DI, Nagaraj S. Myeloid-derived suppressor cells as regulators of the immune system. Nat Rev Immunol. 2009, 9: 162-174.
[5]
Serafini P, Carbley R, Noonan K, Tan G, Bronte V, et al. High-dose GM-CSF-producing vaccines impair the immune response through the recruitment of myeloid suppressor cells. Cancer Res. 2004, 64: 6337-6343.
[6]
Sun LP, Hu YJ, Niyonsaba A, Tong QX, Li HY, Jie SH. Detection and evaluation of immunofunction of patients with Severe Fever with Thrombocytopenia Syndrome. Clin Exp Med. 2014, 14: 389-395.
[7]
Jie SH, Zhou Y, Sun LP, Liang KW, Yi XL, Li HY. Close correlation between development of MODS during the initial 72h of hospitalization and hospital mortality in severe fever with thrombocytopenia syndrome. J Huazhong Univ Sci Technol. 2013, 33: 81-85.
[8]
Sun LP, Li HY, Yi XL, Niyonsaba A, Wang NF, Jie SH. Detection and clinical significance of circulating microvesicles in severe fever with thrombocytopenia syndrome. J infection international. 2013, 2: 49-54.
[9]
Baniyash M. TCR ζ-chain downregulation: curtailing an excessive inflammatory immune response. Nature Reviews Immunology. 2004, 4: 675-687.
[10]
Serafini P, De Santo C, Marigo I, Cingarlini S, Dolcetti L, et al.. Derangement of immune responses by myeloid suppressor cells. Cancer Immunol Immunother. 2004, 53: 64-72.
[11]
Greifenberg V, Ribechini E, Rößner S, Lutz MB. Myeloid-derived suppressor cell activation by combined LPS and IFN-gamma treatment impairs DC development. Eur J Immunol.2009, 39:2865-2876.
[12]
Waight JD, Hu Q, Miller A, Liu S, Abrams SI. Tumor-Derived G-CSF Facilitates Neoplastic Growth through a Granulocytic Myeloid-Derived suppressor cell-dependent mechanism. PLoS One. 2011, 6: e27690.
[13]
Marigo I, Bosio E, Solito S, Mesa C, Fernandez A, Dolcetti L, Ugel S, et al. Tumor-induced tolerance and immune suppression depend on the C/EBPbeta transcription factor. Immunity. 2010, 32: 790-802.
[14]
Trikha P, Carson WE 3rd. Signaling pathways involved in MDSC regulation. Biochim Biophys Acta, 2014, 1846: 55-65.
[15]
Luyckx A, Schouppe E, Rutgeerts O, Lenaerts C, Fevery S, et al.. G-CSF stem cell mobilization in human donors induces polymorphonuclear and mononuclear myeloid-derived suppressor cells. Clin Immunol. 2012, 143: 83-87.
[16]
Gabrilovich DI, Ostrand-Rosenberg S, Bronte V. Coordinated regulation of myeloid cells by tumours. Nat Rev Immunol.2012, 12: 253-268.
[17]
Bronte V, Apolloni E, Cabrelle A, Ronca R, Serafini P, Zamboni P, Restifo NP, Zanovello P. Identification of a CD11b (+)/Gr-1(+)/CD31(+) myeloid progenitor capable of activating or suppressing CD8(+) T cells. Blood. 2000, 96: 3838-3846.
[18]
Marhaba R, Vitacolonna M, Hildebrand D, Baniyash M, Freyschmidt-Paul P, Zöller M. The Importance of Myeloid-Derived Suppressor Cells in the Regulation of Autoimmune Effector Cells by a Chronic Contact Eczema. J Immunol. 2007, 179: 5071-5081.
[19]
Haverkamp JM, Crist SA, Elzey BD, Cimen C, Ratliff TL. In vivo suppressive function of myeloid-derived suppressor cells is limited to the inflammatory site. Eur J Immunol. 2011, 41: 749-759.
[20]
Rodriguez PC, Ernstoff MS, Hernandez C, Atkins M, Zabaleta J, et al.. Arginase I-producing myeloid-derived suppressor cells in renal cell carcinoma are a subpopulation of activated granulocytes. Cancer Res. 2009, 69: 1553-1560.
[21]
Zhuang Y, Peng LS, Zhao YL, Shi Y, Mao XH, et al.. CD8(+) T cells that produce interleukin-17 regulate myeloid-derived suppressor cells and are associated with survival time of patients with gastric cancer. Gastroenterology. 2012, 143: 951-962.
[22]
Heidema J, Lukens MV, van Maren WW, van Dijk ME, Otten HG, et al. CD8+ T cell responses in bronchoalveolar lavage fluid and peripheral blood mononuclear cells of infants with severe primary respiratory syncytial virus infections. J Immunol. 2007, 179:8410-8417.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186