Comprehensive Assessment of Functional Changes in the Organism of Healthy Rats in External and Internal Use of Silicone Low-Mineralized Mineral Water
European Journal of Clinical and Biomedical Sciences
Volume 4, Issue 1, February 2018, Pages: 1-5
Received: Oct. 31, 2017;
Accepted: Dec. 28, 2017;
Published: Jan. 19, 2018
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Sergey Gushcha, Department of Basic Research, SE Ukrainian Research Institute of Medical Rehabilitation and Balneology Ministry of Health of Ukraine, Odessa, Ukraine
Boris Nasibullin, Department of Basic Research, SE Ukrainian Research Institute of Medical Rehabilitation and Balneology Ministry of Health of Ukraine, Odessa, Ukraine
Alexander Plakida, Department of Physical Rehabilitation, Sports Medicine, Physical Training and Valeology, Odessa National Medical University, Odessa, Ukraine
Irina Trubka, Department of Childhood Stomatology, National Medical Academy of Post-Secondary Education, Kiev, Ukraine
Veronika Volyanskaya, Department of Physical Rehabilitation, SE Ukrainian Research Institute of Medical Rehabilitation and Balneology Ministry of Health of Ukraine, Odessa, Ukraine
Nikolay Kalinichenko, Department of Physical Rehabilitation, SE Ukrainian Research Institute of Medical Rehabilitation and Balneology Ministry of Health of Ukraine, Odessa, Ukraine
Irina Balashova, Department of Physical Rehabilitation, SE Ukrainian Research Institute of Medical Rehabilitation and Balneology Ministry of Health of Ukraine, Odessa, Ukraine
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Therapeutic mineral waters (MW) are nonspecific natural modulators; they correct the state of numerous pathogenetic links in the formation of the pathological process in the organism, which allows obtaining long-term therapeutic effects (especially at the initial stages of pathology development). Positive effect from the use of MW is due to their physical and chemical composition: the presence of macro- and microelements and biologically active compounds (sodium, potassium, magnesium, silicon, boron, iron, hydrogen sulfide). MW can be dangerous, because, acting as stress factors of low intensity; they exhaust (with long-term use) adaptive-compensatory mechanisms of the organism and promote the development of disoriginal states. The purpose of the work was to determine the safety and availability of biological activity of thermal silicon low-mineralized water on the state of the organism of healthy white rats at its internal and external application. The influence of MW on the functional state of the nervous system, kidneys, liver and structural and functional organization of the internal organs of white rats was investigated due to the application of physiological, biochemical, histological and statistical methods of research. The experiment was conducted on white rats of the Wistar line, divided into three groups. I group was control, comparative. Animals of II group received silicon MW internally, and animals of group III in the form of external procedures. It was established that the external application of MW causes a more significant calming effect on the functional state of the central nervous system. The emotional state of rats of the third group contrary - activated. The use of MW in animals of group II causes significant stimulation of urinary and excretory renal function. In both groups, the biliary function of the liver is intensified (reducing the total bilirubin content of the blood due to its fractions - direct and indirect bilirubin) and reduces the activity of oxidative-reducing enzymes in the tissues of the stomach, liver and kidneys. Consequently, the use of silicon MW in animals of the II and III groups does not cause harmful or toxic phenomena. The obtained histological results indicate that the animal's organism is in a state of reduced energy supply, that is, it can be assumed that the MW forms the state of adaptation to the damaging factors. In this case, the external application of MW is not less determined than its traditional internal use.
Silicon Mineral Water, Biological Activity, White Rats, Functional State
To cite this article
Comprehensive Assessment of Functional Changes in the Organism of Healthy Rats in External and Internal Use of Silicone Low-Mineralized Mineral Water, European Journal of Clinical and Biomedical Sciences.
Vol. 4, No. 1,
2018, pp. 1-5.
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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Mineral waters of Ukraine / Ed. By Е. О. Кolesnik, К. D. Bабоv.—К.: Кuрrіyanov, 2005, 576 р.
Medical-hydrogeochemical factors of geological environment in Ukraine/ Ed. By H. I. Rud'ko. Kyiv—Chernivtsi: Bukrek, 2015, 724 p.
Nasibullin B. A., Gushcha S. G. Modern ideas about the biological role of silicon in organism of humans and animals//Medical Rehabilitation, Balneology, Physiotherapy.- 2006. No. 1 (45), P. 29-32.
Experimental evaluation of the effect on the organism of the low-mineralized sodium chloride mineral water of the Ketkinsky deposit and the mud extract from the Paratunskoye field / С. В. Калинин // Questions of balneology, physiotherapy and medical physical training.—2002.—№ 6.—P. 35—37.
Kim M. H. Silicon supplementation improves the bone mineral density of calcium-dcficicnt ovaricctomizcd rats by reducing bone resorption / M. H. Kim, Y. J. Bae, M. X. Choi // Biol Trace F. lcm Res. 2009. Vol. I28 (3). Р. 239-247.
A. P. Avcyn, A. A. Zhavoronkov, M. A. Risch, L. S. Strochkova. Microelementoses of man: etiology, classification, organopathology.–М.: Меdicina, 1991.—496 p.
T. A. Zolotareva, B. A. Nasibullin, S. G. Gushcha, N. A. Alexeenko, E. I. Bakholdina, A. Ya. Oleschko. Influence of boric mineral medicinal-table water on the functional state of the liver of experimental animals with chronic alcoholization // Physiotherapy, balneology and rehabilitation.—2014.—№ 1.—P. 4–7.
Pavlova E. S., Bakholdina E. I., Batsko E. S. Influence of mineral waters of various macro-composition and the content of biologically active substances on nonspecific mechanisms of formation of adaptive processors//Medical Rehabilitation, Balneology, Physiotherapy.- 2008, No. 3, P. 19―22.
Tolmacheva N. V. On the role of auto-microflora and microelements in the formation of ecological-physiological features of adaptation.// The success of modern natural science. 2003, No. 5, P. 89-90.
Soetan K. O., Olaiya C. O., Oyewole O. E. The importance of mineral elements for humans, domestic animals and plants: A // African Journal of Food Science. 2010. Vol. 4 (5). Р. 200-222.
Jugdaohsingh R., Simon H. C., Anderson, K. L. Dietary silicon intake and absorption / American Journal Clinical Nutrition. 2002. Vol. 75 (5). Р. 887-893.
A. V. Scalny. Mikroelementozy of human: diagnosis and treatment. 2 nd ed. - М.: КМК,—2001.—96 p.
Nzietchueng R. M., Dousset В., Frank P. Mechanisms implicated in the effects of boron on wound healing / Journal Trace Elements Medical Biology. 2002. Vol. 16 (4). Р. 239–244.
Davenwarda S., Benthamb P., Wrightb J. Silicon-rich mineral water as a non-invasive test of the "aluminum hypothesis’ in alzheimer’s disease // Journal of Alzheimer’s Disease. 2013. Vol. 33. Р. 423–430.
Rehabilitation of victims in conditions of emergencies and hostilities. Post-traumatic stress disorder/Ed. К. Д. Babov, І. Ya. Pinchuk, V. V. Steblyuk.—Оdessa, «Polygraph», 2015—240 p.
Directive 2010/63/ EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (Text with EEA relevance). Official Journal. 2010, L. 276, pp. 0033—0079.
Kozhemyakin Yu. N., Khromov OS., Boldyreva NE., Dobrelya NV., Sayfetdinova GA. Scientific and practical recommendations for the maintenance of laboratory animals and work with them: monograph.— K.: Interservice. 2017: 182 р.
Celis M. E. Measurement of Grooming Behaviour / M. E. Celis, E. Torre/In: Methods in Neurosciences, New York: Academic Press. - 1993. P. 359-378.
Aleksyeyenko N. O., Pavlova O. S., Nasibullin B. A., Ruchkina A. S. Manual methods of research and preformed natural treatment means: natural mineral therapeutic table water and therapeutic water, drinks based on them; artificially mineralized water; peloids, brines, clay, waxes and preparations based thereon. Part 3. Experimental and clinical researches]. Odesa, 2002, 120 p.
Goryachkovsky AM. Clinical biochemistry in laboratory diagnostics. - ed. 3rd. - Odessa: Ecology. 2005–459p.
Order of MH of Ukraine No. 692 from 28.09.2009. «On approval of the methodological recommendations on research methods of biological effects of natural medicinal resources and preformed medicines.
Glants S. Biomedical Statistics / Trans. English.—М.: Praktika. 1999: 459 p.