Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital
European Journal of Clinical and Biomedical Sciences
Volume 3, Issue 6, December 2017, Pages: 101-108
Received: Oct. 4, 2017; Accepted: Oct. 30, 2017; Published: Nov. 15, 2017
Views 1786      Downloads 179
Authors
Abd Elrahman Mustafa Abd Elrahman Osman, Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Shingray Osman Hashim, Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Mohammed Abdall Musa, Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Omer Mohammed Tahir, Medical Laboratory Sciences Division, Port Sudan Ahlia College, Port Sudan, Sudan
Article Tools
Follow on us
Abstract
The presence of ESBLs in many Gram negative strains are of serious concern, since these organisms are the most common cause of different human infections. ESBL positive phenotypically were tested for the presence of ESBL encoding genes using PCR with specific primers for the detection of CTX-M, TEM and SHV genes, then the amplicons were sequenced to characterized gene content. The presence of CTX-M, TEM and SHV genes was confirmed in 65/88 (73.9%) of the isolates. The ESBL genes were detected in 47 Escherichia coli, 14 Klebsiellapneumoniae, 2 Proteus mirabilis, 1 Serratiaodotifera and 1 Enterobactersakasaki. The nucleotide sequences were subjected to BLAST for sequences similarity and homology.
Keywords
E. coli, K. Pneumoniae, ESBLs, CTX-M Genes, TEM Gene, SHV Genes, Sudan
To cite this article
Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir, Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital, European Journal of Clinical and Biomedical Sciences. Vol. 3, No. 6, 2017, pp. 101-108. doi: 10.11648/j.ejcbs.20170306.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Ahmed AA, Osman H, Mansour AM, Musa HA, Ahmed AB, Karrar Z. Antimicrobial agents’ resistance in bacterial isolates from patients with diarrhea and urinary tract infection in the Sudan. The American Journal of Tropical Medicine and Hygiene 2000; 63: 259-263.
[2]
Ahmed, O. B., Omar, A. O., Asghar, A. H., Elhassan, M. M., & Al-Munawwarah, A. M. Prevalence of TEM, SHV and CTX-M genes in Escherichia coli and Klebsiella spp. Urinary Isolates from Sudan with confirmed ESBL phenotype. Life Sci. J 2013; 10(2), 191-195.
[3]
Ajiboye RM, Solberg OD, Lee BM, Raphael E, Debroy C and Riley LW. Global spread of mobile antimicrobial drug resistance determination in human and animal Escherichia coli and Salmonella strains causing community acquired infections: Clinical infection Diseases 2009; 49: 365-371.
[4]
Aktas Z., Kayacan C. B., Schneider I., Can B., Midilli K., Bauernfeind A. Carbapenem hydrolyzing oxacillinase, OXA-48, persists in Klebsiellapneumoniaein Istanbul, Turkey. Chemotherapy 2008; 54(2): 101–106.
[5]
Al Benwan K., Al Sweih N., Rotimi V. O. Etiology and antibiotic susceptibility patterns of community and hospital acquired urinary tract infections in a general hospital in Kuwait. Med. Princ. Pract. 2010; 19(6): 440–446.
[6]
Al-Agamy M. H. Mohamed, El-Din Ashour M. S., Wiegand I. First description of CTXM beta-lactamase-producing clinical Escherichia coli isolates from Egypt. Int. J. Antimicrob. Agents. 2006; 27(6): 545–548.
[7]
Al-azawy, A. F. K. A. S. Humodi."Phenotypic Detection and Molecular Characterization of Extended Spectrum B-Lactamases in Enterobacteriaceae Isolated from Khartoum Hospitals." PhD diss. Sudan Journal of Science and Technology 2012; ISSN: 1858—6716.
[8]
Alsterlund R., Carlsson B., Gezelius L., Haeggman S., Olsson-Liljequist B. Multi resistant CTX-M-15 ESBL-producing Escherichia coli in southern Sweden: description of an outbreak. Scand. J. Infect. Dis. 2009; 41(6–7): 410–415.
[9]
Al-Zahrani, A. J., Akhtar, N. Susceptibility Patterns of Extended Spectrum β-Lactamase (ESBL)-producing Escherichia coli and Klebsiellapneumoniae isolated in a teaching hospital. Pakistan J. Med. Res 2005; 44(2): 64-67.
[10]
Cheesbrough M. District Laboratory Practiceintropical Countries, Part II, Cambridge university press, UK, 2000; chapter 7, pp 36-37-38-167.
[11]
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests; twenty first Informational supplements. CLSI document M100-S21, Wayne, Pa: Clinical and Laboratory Standards Institute 2013; 31(1).
[12]
Coque T. M., Baquero F., Canton R. Increasing prevalence of ESBL-producing Enterobacteriaceae in Europe. Euro. Surveill. 2008; 13(47): 1-11.
[13]
Delela G, GuptaS, Jain DK, Mehta P. Antibiotic Resistance Pattern in Uropathologensis at a Tertiary Care Hospital at Jhalawar with special Reference to ESBL, Ampc b-lactamases and Mrsa Production. J Clin DR 2012; 6(4): 645-651.
[14]
Dereeper A., Audic S., Claverice J. M., Blanc G. BLAST-EXPLORER helps you building datasets for phylogenetic analysis. BMC EvolBiol 2010; (PubMed).
[15]
Moyo SJ, Aboud S, Kasubi M, Lyamuya EF, Maselle SY. Antimicrobial resistance among producers and non-producers of extended spectrum beta-lactamases in urinary isolates at a tertiary Hospital in Tanzania. BMC Res Notes 2010; 3: 348.
[16]
Tschudin-Sutter S, Frei R, Battegay M, Hoesli I, Widmer AF. Extended spectrum β-lactamase-producing Escherichia coli in neonatal care unit. Emerg Infect Dis 2010; 16(11): 1758-1760.
[17]
Al-azawy, A. F. K. A. S. Humodi."Phenotypic Detection and Molecular Characterization of Extended Spectrum B-Lactamases in Enterobacteriaceae Isolated from Khartoum Hospitals." PhD diss. Sudan Journal of Science and Technology 2012; ISSN: 1858—6716.
[18]
Mekki AH, Hassan AN, Elsayed DE. Extended spectrum beta lactamases among multi drug resistant Escherichia coli and Klebsiella species causing urinary tract infections in Khartoum. Journal of Bacteriology Research 2010; 2 (3): 18-21.
[19]
Mohamed Al-Agamy MH, El-Din Ashour MS, Wiegand I. First description of CTX-M beta-lactamase-producing clinical Escherichia coli isolates from Egypt. Int J Antimicrob Agents 2006; 27(6): 545-548.
[20]
Delela G, GuptaS, Jain DK, Mehta P. Antibiotic Resistance Pattern in Uropathologensis at a Tertiary Care Hospital at Jhalawar with special Reference to ESBL, Ampc b-lactamases and Mrsa Production. J Clin DR 2012; 6(4): 645-651.
[21]
Eltayeb HN and Hamedelnil, F. Y. Molecular detection of Extended Spectrum β-lactamases (ESBLs) genes in E. coli isolated from urine specimen, Sennar State, Sudan. International J of advanced scientific and technical research 2012; 5(2): ISSN 2249—9954.
[22]
Kader, A. A., Angamuthu, K, K., Kamath, K. A, and Zaman, M. N. Modified double-disc test for detection of extended-spectrum β-lactamases in Escherichia coli and Klebsiellapneumoniae. British Journal of Biomedical Science 2006; 63: 4; 51-54.
[23]
Dhillon RH, Clark J. ESBLs: A Clear and Present Danger? Crit Care Res Pract 2012; 625170.
[24]
Ahmed AA, Osman H, Mansour AM, Musa HA, Ahmed AB, Karrar Z. Antimicrobial agents’ resistance in bacterial isolates from patients with diarrhea and urinary tract infection in the Sudan. The American Journal of Tropical Medicine and Hygiene 2000; 63: 259-263.
[25]
AminizadehZandKashi MS. Prevalence of multi-drug resistance and pan drug resistance among multiple gram- negative species: experience in one teaching hospital, Tehran, Iran: International Research Journal of Microbiology 2011; 2: 90-95.
[26]
Namboodiri SS, Opintan JA, Lijek RS, Newman MJ and Okeke IN. Quinolone resistance in Escherichia coli from Accra, Ghana: BMC Microbiology 2011; 11: 44.
[27]
Sahuquillo-Arce JM, Selve M, Perpin H, Gobernado M, Armero C, Lopez-Quilez A. Antimicrobial Resistance in More than 100,000 Escherichia coli Isolates According to Culture Site and Patient Age, Gender, and Location: Antimicrobial agents and Chemotherapy 2011; 1222-1228.
[28]
Al-Zahrani, A. J., Akhtar, N. Susceptibility Patterns of Extended Spectrum β-Lactamase (ESBL)-producing Escherichia coli and Klebsiellapneumoniae isolated in a teaching hospital. Pakistan J. Med. Res 2005; 44(2): 64-67.
[29]
Mohammadtaheri Z, Pourpaki M, Mohammadai F, Namdar R and Masjedi MR. Surveillance of Antimicrobial Susceptibility among Bacterial Isolates from Intensive Care Unit Patients of a Tertiary-Care University Hospital in Iran: Chemotherapy 2010; 56: 478-484.
[30]
Moosavian M and Deiham B. Distribution of CTX-M, TEM and SHV gene among ESBL-producing Enterobacteriaceae Isolate in Iran Afr. J Microbiol. Res 2012; 6 (26): 5433-5439.
[31]
Quinteros, M, Radice M, Gardella N, Rodriguez M. M, Costa N, Korbenfeld D, Couto E, and Gutkind G. Extended spectrum beta-lactamases in Enterobacteriaceae in Buenos Aires, Argentina, public hospitals. Antimicrob Agents Chemother 2003; 47: 2864-2867.
[32]
Feizabadi MM, Delfani S, Raji N, Majnooni A, Aligholi M, Shahcheraghi F, Parvin M and Yadegarinia D. Distributionof bla (TEM), bla (SHV), bla (CTX-M) genes among clinical isolates of Klebsiella pneumonia at Labbafinejad Hospital, Tehran, Iran. Microbial drug resistance 2010; 16(1): 49-53.
[33]
Chaibi EB., Sirot D., Paul G. and Labia R. Inhibitor Resistant TEM beta–lactamases: phenotype, genetic and biochemical characteristics. J AntimicrobChemother 1999; 43: 447-458.
[34]
Sekar B, Shwetha R, Arunagiri K, Menaka K, Lalitha P, Aparna V. Detection and characterization of bla CTX-M gene by PCR-RFLP analysis among third generation cephalosporin resistant gram negative isolates. XXX National Congress of Indian Association of Medical Microbiologists. Microconi 2006; P. 27.
[35]
Eltayeb HN and Hamedelnil, F. Y. Molecular detection of Extended Spectrum β-lactamases (ESBLs) genes in E. coli isolated from urine specimen, Sennar State, Sudan. International J of advanced scientific and technical research 2012; 5(2): ISSN 2249—9954.
[36]
Kola A, Holst M, Chaberny IF, Ziesing S, Suerbaums, Gastmeier P. Surveillance of extended-spectrum beta-lactamase-producing bacteria and routine use of contact isolation: experience from a three year period. J Hosp Infect 2007; 66: 46-51.
[37]
Ruppe, E., Hem, S., Lath, S., Gautier, V., Ariey, F., Sarth OV, J. L., Monchy, D., Arlet G. CTX-M β-lactamases in E. coli from community acquired Urinary Tract Infection. Cambodia. EmergInfet Dis 2009; 15 (5): 741-745.
[38]
Hosoglu S, Gundes S, kolayli F, karadenizli A, Demirdag K, Gunaydin M, Altindis M, Caylan R, and Ucmak H. Extended-spectrum beta-lactamases in ceftazidime - resistant Escherichia coli and Klebsiella pneumonia isolates in Turkish hospitals. Indian J Med Microbiol 2007; 25(4): 346-350.
[39]
Eltayeb HN, M. El Amin. Nagwa. Maowia M. Mukhtar. A M Siddig. Mohamed. Molecular Characterization and in Silico Analysis of a Novel Mutation in TEM-1 Beta-Lactamase Gene among Pathogenic E. coli infecting a Sudanese Patient. American Journal of Microbiological Research 2014; 6(2) 217-223.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186