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Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats
European Journal of Clinical and Biomedical Sciences
Volume 2, Issue 6, December 2016, Pages: 69-82
Received: Oct. 19, 2016; Accepted: Nov. 11, 2016; Published: Dec. 12, 2016
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Kouam Foubi Brice Bertrand, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Chuisseu Djamen Dieudonné Pascal, Faculty of Health Sciences, Université des Montagnes, Bangangté, Bangangté, Cameroon
Dzeufiet Djomeni Paul Désiré, Faculty of Sciences, University of Yaoundé I, Yaoundé, Cameroon
Samba Ngano Odette, Faculty of Sciences, University of Dschang, Dschang, Cameroon
Mouelle Sone, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Tiedeu Alain Bertin, National Advanced School of Engineering, University of Yaoundé I, Yaoundé, Cameroon
Gonsu Fotsin Joseph, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
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Ionizing radiations damage cells, tissues and organs among which the liver through a cascade of molecular events that are triggered by reactive oxygen species (ROS), lipid peroxidation and nitrogen species (NS). Aged Garlic Extract (AGE) has been demonstrated to possess free radical scavenging capacity and antioxidant activity. Therefore, the present study has been focused in analyzing the properties of AGE against the lipid peroxidation and oxidative damages of rat liver tissues induced by acute radiation. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. Then, biochemical analyses, lipid peroxidation, total Protein and antioxidants assessment were made from blood samples and liver tissue homogenates. Exposure of rats to gamma radiation caused a significant increase in the level of Malondialdehyde, Nitrite, transaminase enzymes, alkaline phosphatase, and Bilirubin (Total Serum Bilirubin, Direct Bilirubin and Unconjugated Bilirubin) level while a significant decrease was recorded in serum total proteins, glutathione content, superoxide dismutase, catalase activities and total protein level. AGE treated rats revealed a significant improvement in all previous parameters. From these results, it can be concluded that AGE may have significant anti-radiation properties in rat’s liver after radiation exposure.
Irradiation, Oxidative Stress, AGE, Liver, Antioxidant Enzymes, Rats
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Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, Tiedeu Alain Bertin, Gonsu Fotsin Joseph, Protective Effect of Aged Garlic Extract Against the Oxidative Stress Induced by Acute Ionizing Irradiation on Hepatic Antioxidant Enzymes in Rats, European Journal of Clinical and Biomedical Sciences. Vol. 2, No. 6, 2016, pp. 69-82. doi: 10.11648/j.ejcbs.20160206.13
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