Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause
International Journal of Clinical and Experimental Medical Sciences
Volume 3, Issue 3, May 2017, Pages: 30-35
Received: Apr. 29, 2017; Accepted: May 9, 2017; Published: May 19, 2017
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Authors
Mohamad Yosof Rezk, Medical Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Hany Ahmed Elkatawy, Medical Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Ahmed Mohamed Shata, Department of Clinical Pharmacology, Mansoura University, Mansoura, Egypt
Khalid Mohammad Mohany, Biochemistry Department, Faculty of Medicine, Assuit University, Assuit, Egypt
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Abstract
Background: Testosterone facilitates various metabolic processes in the bones, muscles, bone marrow, brain and immune system. Testosterone deficiency may contribute to cardiovascular diseases, central obesity and metabolic dysfunction. Aim: to investigate the effects of testosterone deficiency on blood glucose levels, insulin sensitivity, vitamin D, Homeostatic model assessment of insulin resistance (HOMA-IR), Homeostatic model assessment for beta function (HOMA-IR) and serum lipids in rat models of andropause. Methods, 48 male rats divided into 4 equal groups (n=12/group); a) Control (C) group. b) Orchiectomy (O) group underwent bilateral removal of the testicles. c) Orchiectomy and treated (O+T) group given daily IM testosterone replacement therapy for 7 days, d) Sham and treated (Sh+T) group in which sham operated rats were given the same daily dose of T as O+T group for 7 days. Results: we found that plasma glucose concentration increased significantly (P<0.001) in O group. This increase was corrected by testosterone injection in O+T group. In (Sh+T) group, there is a significant reduction in serum glucose (P<0.001). We also found that insulin level reduced significantly (P<0.001) in O group. This reduction in insulin level was corrected by testosterone. In (Sh+T) group, insulin level increased significantly. 25-OHVD3 was reduced significantly (P<0.05) in O group and increased significantly in O+T group. HOMA-IR level decreased significantly (P<0.001) and this reduction was corrected by Testosterone. HOMA-β reduced significantly (P<0.001) and this reduction was corrected by Testosterone in O+T group. In SH group, there is a marked increase in HOMA-β. We also found insignificant differences between the four groups in serum triglycerides. However, we found very highly significant differences in total cholesterol, LDL and VLDL between C group and O group and very highly significant differences between O+T group and Sh+T group in comparison with O group. We also found significant differences in serum HDL-c in (O) group in comparison with (C) group and between O+T and Sh+T groups in comparison with O group. In conclusion, we found significant reduction in 25-OH(VD), Testosterone, Insulin, HOMA-IR and HOMA-B and significant increase in glucose serum levels in O group and these changes were corrected by Testosterone injection in O+T group. We also found insignificant differences between the four groups in serum triglycerides. However, we found highly significant differences in total cholesterol, LDL, HDL-c and VLDL between C group and O group and very highly significant differences between O+T group and Sh+T group in comparison with O group.
Keywords
Testosterone, Orchiectomized, Insulin, HOMA-IR, HOMA-B, Glucose
To cite this article
Mohamad Yosof Rezk, Hany Ahmed Elkatawy, Ahmed Mohamed Shata, Khalid Mohammad Mohany, Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause, International Journal of Clinical and Experimental Medical Sciences. Vol. 3, No. 3, 2017, pp. 30-35. doi: 10.11648/j.ijcems.20170303.11
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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