Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model
International Journal of Clinical and Experimental Medical Sciences
Volume 1, Issue 3, September 2015, Pages: 60-64
Received: Aug. 12, 2015;
Accepted: Sep. 8, 2015;
Published: Sep. 9, 2015
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Mohammad Rezaeisadrabadi, Yazd Cardiovascular Research Center, Afshar Hospital, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Mohammad Hossein Dashti-Rahmatabadi, Department of Physiology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Shamin Ghobadi, Shahrekord University of Medical Sciences, Shahrekord, Iran
Fatemeh Haddad, Yazd Cardiovascular Research Center, Afshar Hospital, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Shokouh Taghipour Zahir, Department of Pathology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Mansour Ahmadi, Department of Physiology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Mohammad Reza Lotfaliani, Department of Food and Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Aghdas Mirjalili, Department of Histopathology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
Ali Rezaei, Shahrekord University of Medical Sciences, Shahrekord, Iran
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Background. Dexamethasone is a type of steroid medication which enhances the rate of pericyte differentiation and mineralization in vitro with concomitant suppression of calcification inhibitory molecule matrix Gla-protein (MGP). Vitamin K is an essential cofactor in the carboxylation of glutamate residues in a small group of proteins, including MGP. This study tries to assess the efficacy of vitamin K1 on dexamethasone-induced media elastocalcinosis in aorta artery and heart muscle in a rat model. Materials and Methods. 110 male rats with a normal weight range of 270 ± 20 were enrolled in this study. They received a calcification-inducing diet containing both vitamin K1 and dexamethasone during 6 or 12 weeks and were randomly assigned into two groups; a basic group (n=30), and an experimental group (n=80). The experimental group was divided into two groups receiving treatment during 6 and 12 weeks. Administration of dexamethasone was 0.5 mg/kg, intraperitoneal (IP). Vitamin K intakes were different including 5, 10, and 20 µg/kg, which were considered as low, moderate, and high intake, respectively. Results. Plasma concentrations of calcium were not affected by the different regimes and ranged between 2.27 and 2.31 millimolar (mM) (mean ± SD: 2.29 ± 0.02). According to the findings of pathologic biopsy of aorta artery and heart muscle, treatment of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. Conclusion. Administration of 0.5 mg/kg dexamethasone during 6 and 12 weeks did not induce media elastocalcinosis at all. On the other hand, structure and histology of vessels did not change following intake of vitamin K1, therefore, different dosages of vitamin K could not affect the aorta artery status.
Media Elastocalcinosis, Dexamethasone, Vitamin K, Aorta Artery, Heart Muscle
To cite this article
Mohammad Hossein Dashti-Rahmatabadi,
Shokouh Taghipour Zahir,
Mohammad Reza Lotfaliani,
Effect of Phylloquinone on Dexamethasone-Induced Calcification of Heart Muscle and Media Elastocalcinosis in Aorta Artery in Rat Model, International Journal of Clinical and Experimental Medical Sciences.
Vol. 1, No. 3,
2015, pp. 60-64.
Schurgers LJ, Spronk HM, Soute BA, Schiffers PM, DeMey JG, Vermeer C. Regression of warfarin-induced medial elastocalcinosis by high intake of vitamin K in rats. Blood. 2007;109:2823-2831.
Vermer C, Braam L. Role of K vitamins in the regulation of tissue calcification. J Bone Miner Metab. 2001;19:201-206.
Wallin R, Schurgers L, Wajih N. Effects of the blood coagulation vitamin K as an inhibitor of arterial calcification. Thrombosis Research. 2008;122:411-417.
Spronk HM, Soute BA, Schurgers LJ, Thijssen HH, De Mey JG, Vermeer C. Tissue-specific utilization of menaquinone-4 results in the prevention of arterial calcification in warfarin-treated rats. J Vasc Res. 2003;40:531–537.
Thane CW, Paul AA, Bates CJ, Bolton-Smith C, Prentice A, Shearer MJ. Intake and sources of phylloquinone (vitamin K1): variation with socio-demographic and lifestyle factors in a national sample of British elderly people. Br J Nutr. 2002;87:605-613.
McKeown NM, Jacques PF, Gundberg CM, et al. Dietary and nondietary determinants of vitamin K biochemical measures in men and women. J Nutr. 2002;132:1329-1334.
Shanahan CM, Weissberg PL, Metcalfe JC. Isolation of gene markers of differentiated and proliferating vascular smooth muscle cells. Circ Res. 1993;73:193–204.
Davidson RT, Foley AL, Engelke JA, Suttie JW. Conversion of dietary phylloquinone to tissue menaquinone-4 in rats is not dependent on gut bacteria. J Nutr. 1998;128:220-223.
Willenberg T, Diehm N, Zwahlen M, et al. Impact of long-term corticosteroid therapy on the distribution pattern of lower limb atherosclerosis. Eur J Vasc Endovasc Surg. 2010;39:441-446.
Nashel DJ. Is atherosclerosis a complication of long-term corticosteroid treatment? Am J Med. 1986;80:925-929.
Miyahara T, Nemoto M, Tukamoto S, et al. Induction of metallothionein and stimulation of calcification by dexamethasone in cultured clonal osteogenic cells, MC3T3-E1. Toxicol Lett. 1991;57:257-267.
Kirton JP, Wilkinson FL, Canfield AE, Alexander MY. Dexamethasone downregulates calcification-inhibitor molecules and accelerates osteogenic differentiation of vascular pericytes: implications for vascular calcification. Circ Res. 2006;98:1264-1272.
Maas AH, van der Schouw YT, Beijerinck D, et al. Vitamin K intake and calcifications in breast arteries. Maturitas. 2007;56:273-279.
Rees K, Guraewal S, Wong YL, et al. Is vitamin K consumption associated with cardio-metabolic disorders? A systematic review. Maturitas. 2010; 67:121-128.