Anti-diabetic Effect of Myrtenal on Plasma and Tissue Glycoproteins Components in STZ Induced Experimental Diabetic Rats
Journal of Diseases and Medicinal Plants
Volume 2, Issue 1-1, February 2016, Pages: 11-16
Received: Aug. 14, 2015; Accepted: Sep. 1, 2015; Published: Feb. 23, 2016
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Leelavinothan Pari, Phytopharmacology and Molecular Biology Research Laboratory Annamalai University, Annamalainagar Tamilnadu, India; Department of Biochemistry & Biotechnology Faculty of Science, Annamalai University, Annmalainagar, Tamilnadu, India
Ayyasamy Rathinam, Phytopharmacology and Molecular Biology Research Laboratory Annamalai University, Annamalainagar Tamilnadu, India
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The aim of present study was to evaluate the effect of myrtenal, a monoterpene on plasma and tissues glycoprotein components in streptozotocin-induced diabetic rats. Diabetes was induced in overnight fasted experimental rats by a single intraperitoneal injection of streptozotocin (STZ; 40 mg/kg body weight) dissolved in 0.1 M citrate buffer at pH 4.5. STZ-injected animals were given 20 % glucose solution for 24 h to prevent initial drug-induced hypoglycemia mortality. The levels of plasma glucose, plasma and tissues glycoproteins such as hexose, hexosamine, fucose and sialic acid were significantly increased whereas plasma insulin levels were significantly decreased in diabetic rats. On oral administration myrtenal (80 mg/kg b.w.) once daily to diabetic rats for the period of 28 days brought back the levels of plasma and tissues glycoprotein components. Based on the present study, we propose that myrtenal possesses significant protective effect on glycoprotein metabolism.
Myrtenal, Glycoproteins, Wistar Rat, Streptozotocin, Hyperglycemia
To cite this article
Leelavinothan Pari, Ayyasamy Rathinam, Anti-diabetic Effect of Myrtenal on Plasma and Tissue Glycoproteins Components in STZ Induced Experimental Diabetic Rats, Journal of Diseases and Medicinal Plants. Special Issue: Pharmacological Action of Medicinal Plants: Health and Diseases. Vol. 2, No. 1-1, 2016, pp. 11-16. doi: 10.11648/j.jdmp.s.2016020101.12
Ghoul JE, Smiri M, Ghrab S, Boughattas NA, Ben-Attia M. Antihyperglycemic, antihyperlipidemic and antioxidant activities of traditional aqueous extract of Zygophyllum album in streptozotocin diabetic mice. Pathophysiol 2012; 19: 35-42.
Giacco F, Brownlee M. Oxidative stress and diabetic complications Circulation research, 2010; 107: 1058–1070.
Mittal N, Keaur J, Mahmood A. Changes in tubular membrane glycosylation in diabetic insulin and thyroxin treated rat kidneys. Ind J Exp Biol 1996; 34: 782–785.
Knecht KT, Bradford BU, Mason RP, Thurman RG. In vivo formation of a free radicals metabolite of ethanol. Mol Pharmacol 1990; 38: 26–30.
Sharma C, Dalferes FR, Radhakrishnamurthy B, De-Paolo CJ, Berenson GS. Hepatic glycoprotein synthesis in streptozotocin diabetic rats. Biochem Int 1987; 15–19.
Konukoglu D, Serin O, Akcay T, Hatemi H. Relationship between diabetic angiopathic complications and serum total and lipid associated sialic acid concentrations. Med Sci Res 1999; 27: 53–55.
Patti ME, Virkamaki A, Landaker EJ, Kahn CR, Yki-Jarvinen H. Activation of the hexosamine pathway by glucosamine in vivo induces insulin resistance of early post receptor insulin signaling events in skeletal muscle. Diabetes 1999; 48: 1562–1571.
Sundaram R, Naresha R, Shanthi P, Sachdanandam P. Efficacy of 20-OH-ecdysone on hepatic key enzymes of carbohydrate metabolism in streptozotocin induced diabetic rats. Phytomedicine 2012; 19: 725–729.
Tang L, Wei W, Chen L, Liu S. Effects of berberine on diabetes induced by alloxan and a high-fat/high-cholesterol diet in rats J. Ethnopharmacol 2006; 108: 109–115.
Verges B. Lipid modification in type 2 diabetes: the role of LDL and HDL. Fundam Clin Pharmacol 2009; 23: 681–685.
Marica Lindmark H, Dan I, Tomas V, Irena V, Hans-Erik H, Kristina S. Transformation of terpenes using a Picea abies suspension culture. J Biotechnol 2004; 107: 173–184.
Vibha JB, Choudhary K, Mangal Singh, Rathore MS, Shekhawat NS. A study on pharmacokinetics and therapeutic efficacy of Glycyrrhiza glabra a Miracle Medicinal Herb. Bot Res Int 2009; 2: 157–163.
Rathinam A, Pari L, Chandramohan R, Sheikh BA. Histopathological findings of the pancreas, liver, and carbohydrate metabolizing enzymes in STZ-induced diabetic rats improved by administration of myrtenal. J Physiol Biochem 2014; 70: 935–946.
Niebes P. Determination of enzymes and degradation products of glycosaminoglycan metabolism in the serum of healthy and varicose subjects. Clin Chim Acta1972; 42: 399-408.
Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem 1959; 234: 1971-1975.
Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem 1959; 234: 1971-1975.
Dische Z, Shettles LB. A specific color reaction of methylpentoses and a spectrophotometric micro method for their determination. J Biol Chem1948; 175: 595-603.
Shulman GI. Cellular mechanisms of insulin resistance. J Clin Invest 2000; 106: 171–6.
Peng CH, Ker YB, Weng CF, Peng CC, Huang CN, Lin LY et al. Insulin secretagogue bioactivity of finger citron fruit (Citrus medicaL. var. Sarcodactylis Hort, Rutaceae). J Agric Food Chem 2009; 57: 8812–9.
Jeppesen PB, Gregersen S, Poulsen CR, Hermansen K. Stevioside acts directly on pancreatic beta-cells to secrete insulin: actions independent of cyclic adenosine monophosphate and adenosine triphosphate-sensitive K+ channel activity. Metabolism 2000; 49: 208–14.
Parillo F, Arias MP, Supplizi AV. Glycoprofile of the different cell types present in the mucosa of the horse guttural pouches. Tissue Cell 2009; 41: 257-265.
Ciftci G, Yarim GF. Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs. J Vet Sci 2011; 12: 325-331.
Buse MG. Hexosamines, insulin resistance, and the complications of diabetes: current status. Am J Physiol Endocrinol Metab 2006; 290:1-8.
Rahman MA, Zafar G, Shera AS. Changes in glycosylated proteins in long term complications of diabetes mellitus. Biomed Pharmacother 1990; 44: 229–234.
[25] Berenson GS, Radhakrishnamurthy Dalferes ER. Connective tissue macromolecular changes in rats with experimentally induced diabetes and hyperinsulinism. Diabetes 1972; 21: 733–743.
Spiro RG, Spiro MJ. Effect of diabetes on the biosynthesis of the renal glomerular basement membrane. Studies on the glycosyl transferase. Diabetes 1971; 20: 641–648.
Guillot R, Kassab JP, Ogneva U, Andre J, Durussel JJ, Hadjiisky P, Peyroux J, Sternberg M. Relation between pancreatic islet cellular infiltration and plasma fibrinogen or [alpha] 1-acid glycoprotein levels in spontaneously and streptozotocin-diabetic rats: an increase in these protein levels is not necessary for inducing microcirculatory erythrocyte velocity alteration. Pancreas 1994; 9: 336–343.
Robinson KA, Weinstein MP, Lindenmayer GE, Byse MG. Effects of diabetes and hyperglycemia on the hexosamine synthesis pathway in rat muscle and liver. Diabetes 44, 1438–1446.
Bucala, R. Advanced glycosylation end products and diabetic vascular disease, in Keaney JF Jr.(Edn). Oxidative stress and vascular disease. Kluwer academic publishers Dordrecht. 1999; pp. 287-303.
Mondoa S, Emil, Kitei M. 2001. The new healing science of glyconutrients, in sugars that heal. Ballantine Publishing, New York.
Prakasam A, Sethupathy S, Pugalendi KV. Influence of Casearia esculenta root extract on glycoprotein components in streptozotocin diabetic rats. Pharmazie 2005; 60: 229-232.
Latha M, Pari L. Effect of an aqueous extract of Scoparia dulcis on plasma and tissue glycoproteins in streptozotocin induced diabetic rats. Pharmazie 2005; 60: 151-154.
Shanmugasundram ERB, Gopinath KL, Shanmugasundram, KR, Rajendran VM. Possible regeneration of islets of Langerhans in streptozotocin diabetic rats given Gymnema sylvestre leaf extract. J Ethnopharmacol 1990; 30: 265–279.
Kimmelsteil P, Wilson C. Inter capillary lesion in the glomeruli of the kidney. Am J Pathol 1936; 12: 83–105.
Rasch R, Torffucit O, Bachmann S, Jensen PK, Jacobsen NO. Glycoprotein in streptozotocin diabetic rats: a study of kidney in situ hybridization, immunohistochemistry and urinary excretion. Diabetologia 1955; 32: 525–535.
Camerini Davalos RA, Velasco CA, Reddi AS 1990. Metabolism of glomerular basement membrane in diabetes. In: Belfiore, F., Molinatti, G.M., Reaven, G.M. (Eds.), Frontiers in Diabetes. Karger, Basel, Switzerland, pp. 61–77.
Marshall S, Bacote V, Traxinger RR. Discovery of metabolic pathway mediating glucose induced desensitization of glucose transport system: role of hexosamine biosynthesis in the induction of insulin resistance. J Biol Chem 1991; 266: 4706–4712.
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