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Acute Anticonvulsant Effect and Toxicity of Senna tora Leaves Extracts in Seizure Test in Mice
Journal of Diseases and Medicinal Plants
Volume 6, Issue 1, March 2020, Pages: 1-10
Received: Dec. 15, 2019; Accepted: Jan. 7, 2020; Published: Jan. 16, 2020
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Authors
Katoucha Sidoine Zouakeu Ngatcha, Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Cameroon
Elisabeth Ngo Bum, Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Cameroon
Ur Rehman Nisar, Department of Pharmacy, Comsats University Islamabad, Abbotabad-Campus, Pakistan
Nicolas Yanou Njintang, Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Cameroon
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Abstract
Senna tora leaves are used as traditional medicine in Africa for the treatment of many disorders. In Cameroon, the leaves of this plant are used for the treatment of convulsions and malaria. No work has been interested on the anticonvulsant activity and toxicity of Senna tora as affected by solvent extraction. This study has the objective to evaluate the anticonvulsant activity and toxicity of Senna tora extracts. The extracts were produced by maceration in 4 different solvents (water, ethanol, methanol and hydroethanol) and by decoction. Several doses (45, 112.5, 225 and 450 mg / kg) of the five Senna tora extracts were tested for their efficacy against convulsion induced by bicuculin, kainic acid, strychnine and picrotoxin. Normal, negative and positive (phenobarbital and clonazepam treatment) control treatments were equally tested. Firstly, the animal received the treatment (per os), after 1 hour all they animals received the injection of convulsivant solution except normal control. Latency time and duration of convulsion were noted. For acute toxicity, the animals received one dose (5000mg/kg) of the different extracts and were observed during 7 days. As results, it appeared that the methanolic, ethanolic and hydroethanolic extract at the dose 450 mg/kg protected 80% of mice against convulsion induced by strychnine, picrotoxin. In addition, the plant induced a significant decreased of the duration of convulsions. Moreover, no dead nor toxicity were observed for administration doses less than 5000 mg/kg body weight. Definitively, Senna tora extracts exhibit anticonvulsant and are not toxic, thus pointing out the use of the plant in the treatment of epilepsy.
Keywords
Senna Tora, Convulsion, Picrotoxin, Bicuculin, Strychnine, Kainic Acid
To cite this article
Katoucha Sidoine Zouakeu Ngatcha, Elisabeth Ngo Bum, Ur Rehman Nisar, Nicolas Yanou Njintang, Acute Anticonvulsant Effect and Toxicity of Senna tora Leaves Extracts in Seizure Test in Mice, Journal of Diseases and Medicinal Plants. Vol. 6, No. 1, 2020, pp. 1-10. doi: 10.11648/j.jdmp.20200601.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Samanta A, Ganguly S, Hashimoto H, Devadiga S, Vermote E, Knyazikhin Y, Nemani RR, Myneni RB. Amazon forests did not green-up during the 2005 drought. Geophys Res Lett. 2010; 37: doi: 10.1029/2009GL042154.
[2]
Meena AK, Niranjan US, Yadav AK, Singh B, Nagariya AK, Rao MM. Cassia tora Linn, 2010. A review on its ethnobotany, phytochemical and pharmacological profile. J Pharm Res 3 (3): 557-560.
[3]
OMS. (2016). Epilepsy: aetiology, epidemiology, causes and treatments, WHO Fact Sheet, No. 992.
[4]
El-Tahir A, Satti GM, Khalid SA: Antiplasmodial activity of selected sudanese medicinal plants with emphasis on Acacia nilotica. Phytother Res 1999, 13 (6): 474-478.
[5]
Prabhu A, Krishnamoorthy M: antioxidant activity of ethanolic extract of cassia tora L. Int J Res Ayurveda Pharm 2011, 2 (1): 250-252.
[6]
Shaita O: correlation of Hepatoprotective and Antioxidant Effect of Cassia tora Root extract. Master thesis, University of Health Sciences, Bangalore, India, 2005.
[7]
Dhanasekaran M, Ignacimuthu S, Agastian P: Potential hepatoprotective activity of ononitol monohydrate isolated from Cassia tora L. on carbon tetrachloride induced hepatotoxicity in wistar rats. Phytomedicine 2009, 16 (9): 891-895.
[8]
Tzeng TF, Lu HJ, Liou SS, Chang CJ, Lui IM: Reduction of lipid accumulation in white adipose tissues by Cassia tora (Leguminosae) seed extract is associated with AMPK activation. Food Chem 2013, 136 (2): 1086-1094.
[9]
Ngo Bum E., G. S. Taiwe, Moto F. C. O., Ngoupaye G. T., and Nkantchoua G. C. N., Pelanken M. M., Rakotonirina S. V., Rakotonirina A. 2009. Anticonvulsant, anxiolytic, and sedative properties of the roots of Nauclealatifolia Smith in mice. Epilepsy and Behavior, 15: 434-440.
[10]
Ngo Bum E., Dawack L., Schmutz M., Rakotonirina. S. V., Portet C., Olpe. H-R and Herling P., 2005. Anticonvulsant activity of Mimosa pudica decoction. Fitoterapia, 75: 310-315.
[11]
Ngo Bum., G. S. Taiwe., F. C. O. Moto., G. T. Ngoupaye., G. C. N. Nkantchoua., M. M. Pelaken., S. V. Rakotonirina and A. Rakotonirina., 2009b. Anticonvulsivant, anxiolytics and sedative of the roots of Nauclea latifolia Smith in mice. Epilepsie and Behavior, 15: 434-440.
[12]
Ngo Bum, E., Schmutz, M., Meyer, C., Rakotonirina, A., Bopelet, M., Portet, C., Herrling, P. (2001). Anticonvulsant properties of the methanolic extract of Cyperus articulatus (Cyperaceae). Journal of Ethnopharmacology, 76 (2), 145-150.
[13]
Ngo Bum, E., Taiwe, GS., Nkainsa, LA., Moto, FCO., Etet, P. S., Hiana, I. R., Rakotonirina, S. V. (2009a). Validation of anticonvulsant and sedative activity of six medicinal plants. Epilepsy and Behavior, 14 (3), 454-458.
[14]
Nadler JV 2003. The recurrent mossy fiber pathway of the epileptic brain. Neurochemical Research, 28: 1649-1658
[15]
O. E. C. D., Guidance document on acute oral toxicity, Environmental health and safety monograph series on testing and assessment, n° 24, (2000).
[16]
Rang H. P., Dale M. M., Ritter J. M., (2005) Pharmacology. 4e edition. Pp 463-481.
[17]
Karunakar Hegde, Shalin P Thakker, Arun B Joshl, CS Shastry, KS Chzndrzshekhar (2009), Anticonvulsivan Activity of Carissa carandas Linn. Root Extra in Extract in Experimental Mice, Tropical Journal of Pharmaceutical Research, 8 (2): 117-125.
[18]
Mustafa AMS, Ali AM., (2008) Substance in broad beans (Viciafaba) is protective against experimentally induced mice convulsions in mice. Epilepsy Behav. 12, Pp 25-29.
[19]
Ngo Bum E, Nkantchoua GN, Njikam N, Taiwe GS, Ngoupaye GT, Pelaken MM, Nanga, Maidawa F, Rakotonirina A: Anticonvulsivant and sedative activity of leaves of senna spectabilis in mice. Int J pharm 2010, 6 (2): 123-128.
[20]
Perez-saad, Buznego MT. 2008. Behavior and antiepileptic effets of acute administration of extract of the plant Cestrum nocturnum Lin (Lady of the night). Epilepsy Behav; 12: 366-372.
[21]
Ngo Bum E, Ngah E, Mune RN, Minkoulou DZ, Talla E, Moto FCO, Rakotonirina SV: Decoctions of Bridelia micrantha and Croton macrostachyus may have anticonvulsant and sedative effects. Epilepsy Behavior 2012, 24 (3): 319-323.
[22]
Pack A. M., Gidal B. E., 2007. Long term adverse events. In: Engel J. Jr., Pdley T. A., editions. Epilepsy: a comprehensive textbook. 2: 1209-1212.
[23]
Trailovic S. M., and V. M. Varagic, 2007. The effect of invermectin on convulsions in rats producted by lidocaine and strychnine. Vet res. Commun, 363-872.
[24]
Goth A. 1984. Medical Pharmacology, 11th Edition Mosby, St Louis, USA. 815 p.
[25]
Findlay G. S., Wick M. J., Mascia M. P., Wallace D., Millier G. W., Harris R. A. and Blednov Y. A., 2002. Transgenic expression of a mutant glycine receptor decreases alcohol sensitivity of mice. Journal of Pharmacology and Experimental Therapeutics 300, 526-534.
[26]
Gies J-P., 1993. Bases de pharmacologie moléculaire. Edition ellipses, 160 p.
[27]
Cavalheiro, E. A., Fernandes, M. J., Turski, L., Naffah‐Mazzacoratti, M. G. (1994). Spontaneous recurrent seizures in rats: amino acid and monoamine determination in the hippocampus. Epilepsia, 35 (1), 1-11.
[28]
Williams P. A., Hellier J. L., White A. M., Staley K. J and Dudek F. E., 2009. Development of spontaneous seizures after experimental status epilepticus: implications for understanding epileptogenesis. Epilepsia, 48 (5): 157–163.
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