Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains
Journal of Diseases and Medicinal Plants
Volume 5, Issue 6, December 2019, Pages: 103-106
Received: Sep. 23, 2019; Accepted: Nov. 27, 2019; Published: Dec. 7, 2019
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Authors
Faroug Bakheit Mohamed Ahmed, Faculty of Science and Technology, Shendi University, Shendi, Sudan
Abd El-Mohymen Jaber Alla, Faculty of Science and Technology, Shendi University, Shendi, Sudan
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Abstract
The study aimed to detect the antibacterial activity of coriander extract via investigation of six types of bacteria; three gram-positive reference strain were (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), whilst three gram-negative reference strains were (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311 and Pseudomonas aeruginosa ATCC 27853). All bacterial strains were stored in Brain Heart Infusion (BHI) broth with 20% (v/v) glycerol at 280°C. Prior to susceptibility testing, each strain was inoculated on BHI agar to ensure optimal growth and purity plating suitable. Four different concentrations (12.5%, 25%, 50% and 100%) of the oil were dissolved in dimethyl sulfoxide (DMSO) in triplicates using nutrient agar medium. In general, our study revealed that the coriander oil appeared higher influence on the positive gram strain rather than negative gram strain; this might be due to the gram positive strains are more sensitive and potential of hydrophobic essential oils to disrupt the bacterial cell membrane. The results showed that all tested concentrations of coriander oil as antibacterial activity against gram positive and gram negative bacteria; Salmonella typhimurium and Enterococcus fecalis respectively were appeared resistance at the concentrations 12.5%. In addition the concentration 100% inflicted the highest antibacterial activities. The most susceptible bacteria strains was Staphylococcus aureus with highest inhibition zone values (33mm) at concentration 25% and 100% (40mm).
Keywords
Coriander, Extract, Antibacterial, Strain and DMSO
To cite this article
Faroug Bakheit Mohamed Ahmed, Abd El-Mohymen Jaber Alla, Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains, Journal of Diseases and Medicinal Plants. Vol. 5, No. 6, 2019, pp. 103-106. doi: 10.11648/j.jdmp.20190506.13
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Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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