Study on the Optimization of Extraction Technology of Anemonin from Pulsatilla chinensis and Its Inhibitory Effect on Alternaria panax
Journal of Diseases and Medicinal Plants
Volume 5, Issue 6, December 2019, Pages: 94-102
Received: Oct. 21, 2019;
Accepted: Nov. 14, 2019;
Published: Nov. 21, 2019
Views 494 Downloads 159
Xue Fang, Agronomy Department Agricultural College of Yanbian University, Yanji, China
Jinlei Liu, Agronomy Department Agricultural College of Yanbian University, Yanji, China
Lirui Yang, Agronomy Department Agricultural College of Yanbian University, Yanji, China
Xiangguo Li, Agronomy Department Agricultural College of Yanbian University, Yanji, China
Follow on us
Background: The main antibacterial component of Pulsatilla chinensis is anemonin, which has strong antibacterial effect, so it is studied. The Pulsatilla chinensis is one of the diseases that are difficult to solve in the growth of ginseng, but chemical control not only causes harm to the environment, but also has residues on ginseng itself. Pulsatilla is a good medicinal plant. It will have a certain effect on the prevention and treatment of ginseng disease, and it can do no residue, no harm to ginseng. Objective: This experiment can provide effective data support for the prevention and treatment of Pulsatilla chinensis. Method: In this experiment, Pulsatilla Chinensis was used as experimental materials to extract plant by reflux, ultrasonic extraction methods, the content of anemonin in each single factor experiment was determined by HPLC. According to the content, the response surface analysis method was used to find the best extraction process, and the mycelium growth rate method was further used to determine the antifungal effect of Alternaria panax. Results: The EC50 value of P. Chinensis extract to A. panax was methanol extract < acetone extract < ethanol extract in order from small to large; The optimum extraction conditions of anemonin, the effective component of P. Chinensis methanol extract, were as follows: methanol volume fraction 87%, extraction time 2 h, solid-liquid ratio 20 (V: m), the optimum extraction amount was 3.760 μg·g-1.
High Performance Liquid Chromatography, Response Surface Analysis, Anemonin, Alternaria panax
To cite this article
Study on the Optimization of Extraction Technology of Anemonin from Pulsatilla chinensis and Its Inhibitory Effect on Alternaria panax, Journal of Diseases and Medicinal Plants.
Vol. 5, No. 6,
2019, pp. 94-102.
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Cao J H, Li Y L, Qiu S C, et al. The in Vitro Growth Inhibition Effect of Pulsatilla chinensis ( Bge.) Regel (PCR) on Bacteria [J]. Lishizhen Medicine and Materia Medica Research, 2003 (9): 528.
Kim H J, Kim H T, Bae C I, et al. Studies on the hypoglycemic con-stituent of Pulsatillae radix (I) [J]. J Pharm Soc Korea, 1997, 41: 709-713.
Mimaki Y, Yokosuka A, Kuroda M, et al. New bisdesmosidic triter-pene saponins from the roots of Pulsatilla chinensis [J]. Nat Prod, 2001, 64 (9): 1226-1229.
Zhang X Q, Liu A R, Xu L X. Determin ation of ranunculin in Pulsatilla chinensis and Synthetic ranunculin by reversed phase HPLC [J]. Acta Pharmaceutica Sinica, 1990, 25 (12): 932-935.
Ye W C, Qu B X, Ji N N, et al. Patensin, a saponin from Pulsatilla patens var. multifida [J]. Phytochemistry, 1995, 39 (4): 937-939.
Ye W C, Ji N N, Zhao S X, et al. Triterpenoids from Pulsatilla chinensis [J]. Phytochemistry, 1996, 42 (3): 799-802.
Wang B X. Modern Chinese Medicine Pharmacology [M]. Tianjin: Tianjin Science and Technology Press, 1999: 752-755.
Shen X H, Zhang J T, Jiang C, et al. Inhibitory effects of three extracts of pulsatilla on bipolarismaydis [J]. Crops, 2010 (1): 91-93.
Zheng X F, Zhang J Y, Shao S J, et al. Study on the Bacteriostasis of Pulsatilla on Staphylococcus aureus and Enterobacter aerogenes [J]. Journal of Mudanjiang Normal University, 2011 (2): 29-30.
Shi W J, Lu Z X, Li L S. Experimental study on Bacteriostasis of different extracts and compound compounds of Pulsatilla in vitro [J]. Chinese Journal of Traditional Medical Science and technology, 2006 (3): 166-168.
Yang C W. Study on the extraction of saponins from Pulsatilla and their bacteriostasis in vitro [D]. Mudanjiang Normal College, 2016.
Xiong J B. Content determination of anemonin in Ｒanunculus japonicas Thunb. by high-performance liquid chromatography: An experimental study [J]. Hunan Journal of Traditional Chinese Medcine, 2019, 35 (5): 152-154.
Ma D S, Zhang H Q, Sha R, et al. Determination of Anemone in garlic after 7 hours of recovery by HPLC [J]. Journal of Ningxia Medical University, 2017, 39 (2): 237-239.
Zeng Y, Hu X M, Zhai J H. Determination of Anemone in the roots of dahongcao in Wudang Mountain by ion pair chromatography [J]. Hubei Journal of TCM SEP, 2014, 36 (9): 65-66.
Peng Y M, Xiao J, Yang Y. HPLC Determination of anemonin and protoanemonin in different parts of Ranunclus ternatus Thunb by HPLC [J]. Journal of Hunan Univ. of CM, 2013, 33 (9): 55-57, 72.
Zhang H F, Sun J, Teng K. Determination of the Contents of Anemonin in Clematis mandshurica Rupr by HPLC [J]. Chinese Journal of Pharmacovigilance, 2011, 8 (10): 585-586.