In vitro Characterisation of Endophytic Fungi Strains from Lantana camara Leaves Displaying Antifungal Activity Against Phytophthora colocasiae
Journal of Diseases and Medicinal Plants
Volume 5, Issue 6, December 2019, Pages: 87-93
Received: Aug. 31, 2019; Accepted: Oct. 4, 2019; Published: Nov. 21, 2019
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Authors
Mbouobda Hermann Desire, Department of Biology, University of Bamenda, Bamenda, Cameroon; Department of Biological Sciences, University of Yaoundé I, Yaoundé, Cameroon
Muyang Rosaline Forsah, Department of Biology, University of Bamenda, Bamenda, Cameroon
Djeuani Astride Carole, Department of Biological Sciences, University of Yaoundé I, Yaoundé, Cameroon; Department of Plant Biology, University of Yaoundé I, Yaoundé, Cameroon
Djou Tchinda Ismael, Department of Biology, University of Bamenda, Bamenda, Cameroon
Fotso, Department of Biology, University of Bamenda, Bamenda, Cameroon
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Abstract
Colocasiae esculenta is an important tropical tuber crop susceptible to attack by many diseases. The most devastative among these is Taro leaf blight cause by the pathogen Phytophthora colocasiae. The pathogen can cause rapid complete defoliation and crop destruction and under some circumstances, the disease can attack harvested corms and cause heavy losses during storage. Endophytes constitute an important source of bioactive secondary metabolites and enzymes. Based on their phytochemical properties, they can be used as a source of antifungal agent for the treatment of some infectious diseases. In order to evaluate the impact of endophytes on plant defence, in vitro evaluation of the growth effect of endophytic fungi against P. colocasiae was conducted in dual culture, after isolating and screening endophytic fungi from L. camara leaves for their production of some extracellular enzymes (amylases, lipases, laccases, protease and cellulose) and some secondary metabolites (tannins, saponins, phenols, cardiac glycoside) using standard procedures. In-vitro culture techniques with Potato Dextrose Agar (PDA) as culture medium were used to isolate endophytes from L. camara leaf tissues. Isolate identification was done using macroscopic and microscopic characteristics. These isolates were then tested in vitro to evaluate their morphological growth effect against P. colocasiae via the dual culture. Five endophytic fungi were isolated from Lantana camara leaves and coded L1, L2, L3, L4 and L5. The L2, L3 and L5 strains were filamentous and showed coenocytic hyphae which bore some structures’ called conidiosphores identified as Cladosporium sp. L1 strain was filamentous fungi having clamp connections hyphae (bridge-like growth) above hyphal septa and brown sclerotia. L4 strain showed very tiny interwoven and tightly parked mycelia. As enzyme activities, all the strains were found to produce amylase and not protease. L1, L3 and L4 were able to synthesis laccase while L5 produced cellulose and lipase. For the secondary metabolites, all the strains were able to produced tannins and cardiac glycoside but they did not synthesis saponins. L1, L2 and L4 strains were able to produced phenol. In dual culture, the growth of the pathogen decreased the growth rate of the endophytes. L2 strain grew normally by forming an arc around P. colocasiae growth while L3 and L5 strains induced the pathogen to grow on the opposite side of P. colocasiae. Concerning L1 and L4 strains, there were no effect. Based on the antifungal activities against P colocasiae, these strains L2 and L3 could be used for biological control of taro life blight.
Keywords
Lantana camara, Clasdosporium sp., Phenol, Laccase, Phytophthora colocasiae
To cite this article
Mbouobda Hermann Desire, Muyang Rosaline Forsah, Djeuani Astride Carole, Djou Tchinda Ismael, Fotso, In vitro Characterisation of Endophytic Fungi Strains from Lantana camara Leaves Displaying Antifungal Activity Against Phytophthora colocasiae, Journal of Diseases and Medicinal Plants. Vol. 5, No. 6, 2019, pp. 87-93. doi: 10.11648/j.jdmp.20190506.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Onwueme I C. Tropical root and tuber crops - Production, perspectives and future prospects. FAO Plant Production & Protection, Rome, 1994. 228 pp.
[2]
Mbong G A, Fokunang C N, Lum FA, et al. An overview of Phytophthora colocasiae of cocoyams: A potential economic disease of food security in Cameroon. Discourse Journal of Agriculture and Food Sciences, 2013, 1 (9): 140-145.
[3]
Davinder S, Grahame J, Hunter D, et al. A Threat to Food Security: Agriculture, 2012, 2 (3), 182-203.
[4]
Qian Li, Rongjun Guo, Yujia Li, Wyatt H. Hartman, Shifang Li, Zhixiang Zhang, Susannah G. Tringe, Hongqing Wang. Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance. Appl. and Environ. Microbiol., 2019, 85 (9).
[5]
Ruby E J, Bhavna VM and Raghunath T M. A review: Natural products from plant associated endophytic fungi. J. Microbiol. Biotech. Res., 2011, 1 (2): 21-32.
[6]
Arnold AE., Mejia LC., Kyllo D, et al. Fungal endophytes limit pathogen damage in a tropical tree. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100: 15649-15654.
[7]
Mbouobda HD, Fotso, Muyang RF, et al. Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. Leaves. Journal of Applied Biology and Biotechnology, 2014, 2 (06): 01-06.
[8]
Sanjeeb K, Gaurav K, Loganathan K, et al. A Review on Medicinal Properties of Lantana camara Linn. Research Journal of Pharmacy and Technology, 2012, 5 (6): 711-715.
[9]
Latha P, Anand T, Ragupathi N, et al. Antimicrobial activity of plant extracts and induction of systemic resistance in tomato plants by mixtures of PGPR strains and Zimmu leaf extract against Alternaria solani. Biology Control, 2009, 50: 85-93.
[10]
Chung S, Kong H, Buyer JS, et al. Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper. Apply Microbiology Biotechnology, 2008, 80: 115-123.
[11]
Williams (2001) in: James B. Pawley Handbook of Biological Confocal Microscopy, third edition. 125P.
[12]
Hankin L. and Anagnostakis S L. The use of solid media for detection of enzyme production by fungi. Mycology, 1975, 67: 597-607.
[13]
Stone J K, Polishook J D, White J R J. Endophytic fungi. In: G. Mueller, G. F. Bills and M. S. Foster (eds.), Biodiversity of fungi: inventory and monitoring methods, Elsevier, Burlington, MA, USA. 2004, 241–270.
[14]
Shankar N B, Shashikala J, Krishnamurthy Y L. Diversity of fungal endophytes in shrubby medicinal plants of Malnad region, Western Ghats, southern India. Fungal Ecology, 2008, 1: 89-93.
[15]
Hayfa Jabnoun-Khiareddine, Nesrine Ibrahim, Rania Aydi Ben A., Messaoud M. and Mejda Daami-Remadi. Response of Tunisian Pomegranate (Punica granatum L.) Cultivars and Several Plant Hosts to Coniella granati (Saccardo). J. Horti., 2018, 5: 4.
[16]
Figen Mert-Tÿrk. Saponins versus plant fungal pathogens. Journal of Cell and Molecular Biology, 2006, (13) 5: 13-17.
[17]
Hari O, Anjana S, Nasser M. et al. Phytochemical screening and elemental analysis in different plant parts of Uraria picta Desv: A Dashmul species. Journal of chemistry and pharmaceutical Research, 2014, 6 (5): 756-760.
[18]
Lorena Martínez, Julián Castillo, Gaspar Ros and Gema Nieto. Antioxidant and Antimicrobial Activity of Rosemary, Pomegranate and Olive Extracts in Fish Patties. Antioxidants 2019, 8, 86; doi: 10.3390/antiox8040086.
[19]
Sunitha V, Nirmala D, and Srinivas C. Extracellular Enzymatic Activity of Endophytic Fungal Strains Isolated from Medicinal Plants. World Journal of Agricultural Sciences, 2013, 9 (1): 01-09.
[20]
Kavitha K. N., Ganesh S. G. and Sanjeev A. K. Enhanced biodegradation and kinetics of anthraquinone dye by laccase from an electron beam irradiated endophytic fungus. International Biodeterioration & Biodegradation, 2018, 132: 241-250.
[21]
Maria GL, Sridhar KR, Raviraja NS. Antimicrobial and enzyme activity of mangrove endophytic fungi of southwest coast of India. Journal of Agricultural Technology, 2005, 1: 67-80.
[22]
Orlandelli R, Tiago TD, Raiani NA, et al. Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw. Braz J Microbiol, 2015, 46 (2): 359–366.
[23]
Abdel-Raheem A, Shearer C A. Extracellular enzyme production by freshwater ascomycetes. Fungal Divers, 2002, 11: 1-19.
[24]
Omri MF, Castillo G, Parades SH, et al. Understanding and exploiting plant beneficial microbes. Current opinion in Plant Biology. 2017, 38: 155-163.
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