Chemical Constituents of Clerodendrum splendens (Lamiaceae) and Their Antioxidant Activities
Journal of Diseases and Medicinal Plants
Volume 4, Issue 5, October 2018, Pages: 120-127
Received: Oct. 11, 2018; Accepted: Oct. 29, 2018; Published: Nov. 21, 2018
Views 332      Downloads 53
Nganso Ditchou Yves Oscar, Department of Chemistry, Faculty of Science, University of Maroua, Maroua, Cameroon
Tatsimo Ndendoung Simplice Joel, Department of Chemistry, Higher Teachers’ Training College, University of Maroua, Maroua, Cameroon
Amang A. Ngoung Gabrielle Ange, Department of Chemistry, Faculty of Science, University of Maroua, Maroua, Cameroon
Soh Desire, Department of Organic Chemistry, Higher Teachers’ Training College, University of Bamenda, Bamenda, Cameroon
Simo Nemg Fredy Brice, Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Nyasse Barthelemy, Department of Organic Chemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Article Tools
Follow on us
The purpose of this study was to evaluate the antioxidant activity of compounds isolated from Clerodendrum splendens leaves. The leaves of Clerodendrum splendens are used in traditional medicine by indegenous people to treat shingles, spleen in children, asthma, rheumatism, ulcers and malaria. In vivo and in vitro studies carried out by many researchers have shown that Clerodendrum splendens has antioxidant properties. The chemical study of the methanol extract of Clerodendrum splendens leaves (Lamiaceae) led to the isolation of three compounds: Triancontanol (1), (22E, 24S) - Stigmasta - 5, 22, 25 - trien – 3β-ol(2); 3-O-D-glucopyranoside of (22E, 24S) - Stigmasta - 5,22,25 - trien - 3β-ol (3). Their structures were elucidated on the basis of a spectroscopic analysis and a comparison of their data spectral with those reported in the literature. The results of the antioxidant activity have shown that the compounds 1 and 2 inhibit the peroxidation of the hepatic lipids, they also show that the compounds 1, 2 and 3 have a reducing effect on Fe2+. However, the compounds 1, 2 and 3 have an OH reduction power which is directly proportional to the concentration of these compounds compared to that of vitamin C, which made it possible to determine the IC50 of the different compounds. Furthermore, the compounds 1 and 2 have higher IC50 than that of vitamin C (5.613 ± 0.117). The results of this study suggest that Clerodendrum splendens represents an untapped source of compounds with potential antioxidant activity that could be explored in the development of new therapeutic natural products.
Clerodendrum splendens, Lamiaceae, Antioxidant Activities, Secondary Metabolites
To cite this article
Nganso Ditchou Yves Oscar, Tatsimo Ndendoung Simplice Joel, Amang A. Ngoung Gabrielle Ange, Soh Desire, Simo Nemg Fredy Brice, Nyasse Barthelemy, Chemical Constituents of Clerodendrum splendens (Lamiaceae) and Their Antioxidant Activities, Journal of Diseases and Medicinal Plants. Vol. 4, No. 5, 2018, pp. 120-127. doi: 10.11648/j.jdmp.20180405.11
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Falkenberg M, Gaspari M, Rantanen A, Trifunovic A, Larsson NG, Gustafsson CM (2002). Mitochondrial transcription factors B1 and B2 activate transcription of humanmt DNA. Nat Genet.: 289-294.
Berhaut J (1975). Flore illustrée du Sénégal. Dicotylédones. Tome III Connaraceae and Euphorbiaceae. Dakar, 3: 603-604.
Oppong A, (‎2014). Phytochemical examination of the stem of Clerodendrum serratum (L) Moon. Indian Drugs. 4: 208-210.
Nnanga EN, Pouka CK, Boumsong PCN, Dibong S, Didier, Mpondo, ME (2016). Inventaire et caractérisation des plantes médicinales utilisées en thérapeutique dans le département de la Sanaga Maritime: Ndom, Ngambe et Pouma. Journal of Applied Biosciences 106: 10333–10352.
Okwu D, Iroabuchi F (2009). Phytochemical composition and biological activities of Uvaria chamae and Clerodendoron splendens. Ethnobotanical Journal Chemistry, 6: 553–560.
Amal F (2014). Pharmacognostical study of Clerodendrum splendens G. Don. A Thesis Submitted for the Master Degree in Pharmaceutical Sciences: 307-310.
Emelia K (2008). Antimicrobial and Wound Healing Activities of Clerodendron splendens G. Don. PhD thesis, Faculty of pharmacy and pharmaceutical sciences college of health sciences Kumasi, Ghana: 101.
Gbedema S, Kisseih E, Adu F, Kofi A, Woode E (2010). Wound healing properties and kill kinetics of Clerodendron splendens G. Don, a Ghanaian wound healing plant. Pharmacognosy Research, 2: 63–68.
Rohitash C, Mengjie Z, Lifeng P (2012). Application of Cooperative Convolution Optimizationfor 13C Metabolic Flux Analysis: Simulation of Isotopic Labeling Patterns Based on Tandem Mass Spectrometry Measurements: 178–187.
Mac DWlK, Wood LG, Garg ML (2006). Methodology for the determination of biological antioxidant capacity in vitro; a review. J Sci. Food Agric, 86: 2046-2056.
Moon JK, Shibamoto T (2009). Antioxidant assays for plant and food components. Journal of agricultural and Food Chemistry, 57(5): 1655-1666.
Zhenwei S, Xinliang ZSH, Loukin W, John H, Yoshiro S, Ching K (2009). The use of yeast to understand TRP-channel mechanosensitivity. PflügersArchiv - European Journal of Physiology, 458(5): 861–867.
John J S, Peter T, Lac In, Lu A, Liu, Samuel OM, Victoria AK, Dean AK, Scott A, Rasgon (2010). Vitamin D deficiency and anemia: a cross-sectional study. Annals of Hematology 89(5): 447–452.
Yin H, Xu L, Porter NA (2011) Free radical lipid peroxidation: mechanisms and analysis. Chemical Reviews, 111: 5944-5972.
Antonio A, Mario F, M, Sandro A (2014). Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Medicine and Cellular Longevity: 31.
Toshihiro A, Toshitake T, Taro M (1990). ‹‹(22Z, 24S)-Stigmastra-5,22,25-trien-3β-oland Other Novel Sterols from Clerodendrum scandens: first report of the Isolation of a cis-∆22 – Unsaturated Sterol from a Higher Plant›› Journal of chemistry society of Perkin Trans, 1: 2013-2018.
Hisashi K, Noriko S, Akiko H, Haruo O (1990). ‹‹sterol glucosides from frunella vulgaris››. Pergamon Press, 29(7): 2351- 2355.
Das SC, Qais MN, Kuddus MR, Hasan CM (2013). Isolation and Characterization of (22E, 24S)-Stigmasta-5,22,25-trien-3β-ol from Clerodendrum viscosum Vent. Asian Journal of Chemistry, 25(11): 6447-6448.
Sakinah HSS, Noor AMY, Shahrul BK, Philip PJH (1992). The Magnitude of Malnutrition among Hospitalized Elderly Patients in University Malaya Medical Centre. Health and the Environment Journal, 1(2): 64-72.
Pilipenko VA (1990). ULF waves on the ground and in space. Journal of Atmospheric and Terrestrial Physics, 52(12): 1193-1209.
Iris FF, Benzie JJ, Strain (1996). The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239(1): 70-76.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186