Evaluation of Antidiabetic Activity of Aqueous Extract of Leaves from Phragmanthera capitata (Sprengel) S. Balle (Laurenthaceae) in Wistar Albino Rats
Journal of Diseases and Medicinal Plants
Volume 4, Issue 4, August 2018, Pages: 96-109
Received: Aug. 23, 2018; Accepted: Sep. 7, 2018; Published: Nov. 9, 2018
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Authors
Cesaire Feudjio, Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Guy Sedar Singor Njateng, Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Jules-Roger Kuiate, Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
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Abstract
Diabetes mellitus is a public health problem affecting about 8.4% of people in the world and knows an alarming progression in sub-Saharan Africa and in Cameroon precisely. The current therapeutic strategies have prohibitive prices, are unavailable and have harmful side-effects. So, this work aimed at contributing to the valorization of medicinal flora and `knowledge of traditional healers in Foumban, West-Cameroon, in the treatment of diabetes. An ethnopharmacological survey was conducted by consulting traditional healers of the District of Foumban. At the end of this investigation, Phragmanthera capitata, which was found to be the most active plant following preliminary tests, was selected for antidiabetic studies on three groups of rats: normal glycaemia, temporary hyperglycemia and diabetic. The first two groups were treated per os, with aqueous extract of leaves from P. capitata, with the doses of D2 (4 mL/kg) obtained from the traditional healers, D1 (half of D2), D3 (double of D2) and by glibenclamide (2.5 mg/kg). The rats of the last group were treated twice per day, with the same doses of extract and glibenclamide for 13 days and were sacrificed on the 14th day. Their blood was collected by cardiac puncture for the determination of biochemical and hematological parameters. Phytochemical screening was performed using standard methods. The administration of aqueous extract of P. capitata led to a hypoglycaemia effect in normal rats, a reduction of glycaemia in the temporary hyperglycaemia and diabetic rats. In addition, an improvement of lipid assessment, renal and hepatic parameters related to the installation of diabetes, as well as an immune-modulator effect of the extract were noted in diabetic rats treated for 13 days. Phytochemical screening indicated that the extract contains saponins, tannins, flavonoids, steroids and terpens. This work along with findings justifies the traditional use of this plant in the treatment of diabetes mellitus.
Keywords
Diabetes, Ethnopharmacologic Survey, Phragmanthera capitata, Biochemical Parameters, Hematologic Parameters
To cite this article
Cesaire Feudjio, Guy Sedar Singor Njateng, Jules-Roger Kuiate, Evaluation of Antidiabetic Activity of Aqueous Extract of Leaves from Phragmanthera capitata (Sprengel) S. Balle (Laurenthaceae) in Wistar Albino Rats, Journal of Diseases and Medicinal Plants. Vol. 4, No. 4, 2018, pp. 96-109. doi: 10.11648/j.jdmp.20180404.11
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Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
FID. Atlas du diabète de la FID. 6e édition, 2014.
[2]
Tsabang N, Guedje NM, Nnanga J, Tamzé V, Biyiti L, Agbor T, Agbor G, Donfangsiteli N, Kinga J, Essamé OJL. Production des médicaments traditionnels améliorés au Cameroun: cas d’un hypoglycémiant oral. Health sciences and disease. 2012; 12:1-9.
[3]
Mbanya NJC, Motala AA, Sobngwi E, Assah KF, Enoru TS. Diabetes in sub-SaharanAfrica. Seminar. 2010; 375:2254-6.
[4]
Colagiuri S, Colagiuri R, Na’ati S, Muimuiheata S, Hussain Z, Palu T, The prevalence of diabetes in the Kingdom of Tonga. Diab. Care. 2002; 25:1378-83.
[5]
Mannan A, Rupa AB, Azam KN, Nasir Ahmed N, Hasan N. A Quick Review on Anti-diabetic Plants and Action of Phytochemicals. Intern. J. Ad. Res. 2014; 2:227-9.
[6]
Sy GY, Barbosa FS, Wele A, Gueye PM, Gueye CD, Cisse A, Dieye AM, Bassene E, Faye B. Activité anti-hyperglycemiante de la fraction F2 de l’extrait total acétonique de feuilles de vernonia colorata (composeae). Pharm. Méd. Trad. Africaines. 2008; 15:6-10.
[7]
Chabane D, Saidi F, Rouibi A, Azine K. Activité hypoglycémique de l’extrait aqueux d’AjugaivaL. schreber chez les rats diabétiques induite par l’alloxane. Afrique Science. 2013; 9:120-7.
[8]
Gning SB, Thiam M, Fall F, et al. Le diabète sucré en Afrique subsaharienne: aspects épidémiologiques, difficultés de prise en charge. Med. Trop. 2007; 67:607-11.
[9]
Okigbo RN, Nmeka IN. Control of Yam tuber rot with leaf Extracts of Xylopiaaethiopica and Zingiberoffinale. African J. Biotechnol. 2005; 4:804-7.
[10]
Okigbo RN, Omodamiro OD. Antimicrobial effect of leaf extract of pigeon pea (Cajanuscajan (L) Mill sp) on some human pathogen. J. Herbs, spices Med. Plants. 2006; 12:117-27.
[11]
Fleurentin, J. L’ethnopharmacologie au service de la thérapeutique: sources et méthodes. Hegel. 2012; 2:12-8.
[12]
WHO. Stratégie de l’OMS pour la médecine traditionnelle pour 2014–2023. Genève; 2013.
[13]
Noumi E, Yumdinguetmun R. Plants and treatment of prostatic diseases in Foumban (West Region, Cameroon). Syllabus Review. 2010; 2:9-16.
[14]
Trotter RT, Logan MH. Informant consensus: a new approach for identifying potentially effective medicinal plants. In: Etkin, N. L. (Ed.), Plants in Indigenous Medicine and Diet, Behavioural Approaches. Redgrave Publishing Company. Bredford Hills. New York: 1986. p. 91–112.
[15]
Telefo PB. Contribution à l’étude des plantes médicinales du Cameroun: Influence de l’extrait aqueux du mélange des feuilles d’Aloe buettneri A. Berger (Liliacées), Dicliptera verticillata GJH Amshoff (Acanthacees), Hibiscus macranthusHochst ex Arich (Malvacees), Justicia insularis T. Anders (Acanthacées), sur certains paramètres biochimiques et physiologiques de la reproduction chez la rate. Thèse de Doctorat 3e cycle en Biochimie. Université de Yaoundé I, Cameroun; 1998.
[16]
Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiol. Res. 2001; 50:536-46.
[17]
Ndomou M, Djidjou KP, Ayong NM, Gouado I, Tchiegang C. Evaluation de l’activité antidiabétique des extraits de feuilles de Gnetum africanum et Gnetum bulchozzianum (Gnétacées). Sciences, Technologies et Développement. 2014; 15:60-5.
[18]
Bruneton J. Pharmacognosie, Phytochimie, Plantes médicinales. 3ème Edition. Lavoisier. Paris; 1999.
[19]
Klotoé JR, Dougnon TV, Koudouvo K, Atègbo JM, Loko F, Akoègninou A, Aklikokou K, Dramane K, Gbeassor M. Ethnopharmacological survey on antihemorrhagic medicinal plants in South of Benin. Eur. J. Med. Plants. 2013; 3:40-51.
[20]
Cheikhyoussef A, Shapi M, Matengu K, Ashekele HM. Ethnobatanical study of indigenous knowledge on medicinal plant use by traditional healers in Oshikoto region, Namibia. J. Ethnobiol. Ethnomed. 2011; 7:1-11.
[21]
Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1995; 2:137-89.
[22]
Tra-BI FH, Irié GM, N’gaman KCC, Mohou CHB. Études de quelques plantes thérapeutiques utilisées dans le traitement de l’hypertension artérielle et du diabète: deux maladies émergentes en Côte d’Ivoire. Sciences & Nature. 2008:5:39-48.
[23]
Azzi R, Djaziri R, Lahfa F, Sekkal FZ, Benmehdi H, Belkacem N. Ethnopharmacological survey of medicinal plants used in the traditional treatment of diabetes mellitus in the North Western and South Western Algeria. Journal of Med. Plants Res. 2012; 6:2041-50.
[24]
Fah L, Klotoé JR, Dougnon V, Koudokpon H, Fanou VBA, Dandjesso C, Loko F. Étude ethnobotanique des plantes utilisées dans le traitement du diabète chez les femmes enceintes à Cotonou et Abomey-Calavi (Bénin). J. Anim. Plant Sci. 2013; 18:2647-58.
[25]
Orch H, Douira A, Zidane L. Étude ethnobotanique des plantes médicinales utilisées dans le traitement du diabète, et des maladies cardiaques dans la région d’Izarène (Nord du Maroc). J. Applied Biosci. 2015; 86:7940-56.
[26]
Kone D. Enquête ethnobotanique de six plantes médicinales maliennes - extraction, identification d’alcaloïdes - caractérisation, quantification de polyphénols: étude de leur activité antioxydante. Thèse de Doctorat en Chimie Organique. Université de Bamako, Mali; 2009.
[27]
Azwanida NN. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Med. Arom. Plants. 2015; 4:1-6.
[28]
N’Guessan K, Tra-Bi FH, Koné MW. Étude ethnopharmacologique de plantes antipaludiques utilisées en médecine traditionnelle chez les Abbey et Krobou d’Agboville (Côte d’Ivoire). Ethnopharmacologia. 2009; 44:42-50.
[29]
Kambouche N, Merah B, Derdour A, Bellahouel S, Bouayed J, Dicko A, Younos C, Soulimani R. Hypoglycemic and antihyperglycemic effects of Anabasis articulate (Forssk) Moq (Chenopodiaceae), an Algerian medicinal plant. African J. Biotechnol. 2009; 8:5589-94.
[30]
Koneri RB, Samaddar S, Ramaiah CT. Antidiabetic activity of triterpenoidsaponin isolate of MomordicacymbalariaFenzl. Indian J. Exp. Biol. 2014; 52:46-52.
[31]
Velayutham R, Sankaradoss N, Nazeer-Ahamed KFH. Protective effect of tannins from Ficus racemosa in hypercholesterolemia and diabetes induced vascular tissue damage in rats. Asian Pacific J. Trop. Med. 2012:367-73.
[32]
Hi CST, Howell SL. Effects of flavonoids on insulin secretion and calcium handling in rat islet of langerhans. J. Endocrinol. 1995:1-8.
[33]
Waltner-Law ME, Wang XL, Law BK, Hall RK, Nawano M, Granner DK. Epigallocatechingallate, a constituent of green tea, represses hepatic glucose production. J. Biol. Chem. 2002; 277:34933-40.
[34]
Shimizu M, Kobayashi Y, Suzuki M, Satsu H, Miyamoto Y. Regulation of intestinal glucose transport by tea catechins. Biofactors. 2000; 13:61-65.
[35]
Li WL, Zheng HC, Bukuru J, De Kimpeb N. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J. Ethnopharmacol. 2004; 92:1-21.
[36]
Medjdoub H, Selles C, Tabti B. Medicinal plants: a methodology for studying their anti-diabetic activity. Intern. J. Med. Pharm. Sci. 2013; 3:171-8.
[37]
Sarkhail P, Rahmanipour S, Fadyevatan S, Mohammadirad A, Dehghan G, Amin G, Shafiee A, Abdollahi M. Antidiabetic effect of Phlomis anisodonta: Effects on hepatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes. Pharmacol. Res. 2007; 56:261-66.
[38]
Mogale MA, Lebelo SL, Thovhogi N, de Freitas AN, Shai LJ. α-Amylase and α-glucosidase inhibitory effects of Sclerocarya birrea [(A. Rich.) Hochst.] subspecies caffra (Sond) Kokwaro (Anacardiaceae) stem-bark extracts. African J. Biotechnol. 2011; 10:15033-39.
[39]
Onal S, Timur S, Okutucu B. Inhibition of alpha-glucosidase by aqueous extracts of some potent antidiabetic medicinal herbs. Prep. Biochem. Biotechnol. 2005; 35:29-36.
[40]
Gray AM, Flatt PR. Insulin-secreting activity of the traditional antidiabetic plant Viscum album (mistletoe). J. Endocrinol. 1999; 160:409-14.
[41]
Henquin JC, Gilon P. Le contrôle de la sécrétion d'insuline par le glucose: signaux déclenchants et amplificateurs. Méd./sci. 1995; 11:1235-42.
[42]
Ziaei A, Ramezani M, Wright L, Paetz C, Schneider B, Amirghofran Z. Identification of spathulenol in Salvia mirzayanii and the immunomodulatory effects. Phytother. Res. 2011; 25:557-62.
[43]
Khanna K, Rizvi F, Chander R. Lipid lowering activity of phyllanthusnirui in hyperlipipemic rats. J. Ethnopharmacol. 2002; 82:19-22.
[44]
Orskov H, Olsen TS, Wielsen K, Rafael OJ, Lundbeak KK. Kidney lesions in rats with severe long terme alloxan. Diabetologia. 1965; 1:172-9.
[45]
Bouhouche I. Etude comparative de l’alloxane et de la streptozocine dans le diabète expérimental chez le rat blanc. Etude histologique du pancréas endocrine et la variation des paramètres sanguins. Mémoire de Magister en Biologie Animale. Université Constantine 1, Algérie; 2015.
[46]
Babu PVA, Sabitha KS, Shyamaladevi CS. Therapeutic effect of green tea extract on oxidative stress in aorta and heart of streptozotocin diabetic rats. Chemico-Biol. Interact. 2006; 162:114-20.
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