Proximate, Phytochemical and Antibacterial Analysis of Persea americana Obtained from Nigeria
Journal of Diseases and Medicinal Plants
Volume 4, Issue 3, June 2018, Pages: 89-95
Received: Jun. 9, 2018; Accepted: Jul. 30, 2018; Published: Sep. 19, 2018
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Umeaku Chinyelu Nkiru, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Anambra, Nigeria
Chris-Umeaku Chiamaka Ijeoma, Department of Biochemistry, Chukwuemeka Odumegwu Ojukwu University, Anambra, Nigeria
Emmy-egbe Ifeyinwa Orsla, Department of Biological Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra, Nigeria
Ukoha Chinwe Clarice, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Anambra, Nigeria
Uzor Ugonwa Chiagoziem, Department of Mass Communication, Chukwuemeka Odumegwu Ojukwu University, Anambra, Nigeria
Agbo Uchenna Juliet, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Anambra, Nigeria
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The proximate analysis and antimicrobial activities of Persea Americana seed (Avocado pear) against pathogenic Escherichia coli and Staphylococcus aureus was carried out to ascertain the unique properties that makes it confer antibacterial effect. This research work was carried out in the Microbiology Project Laboratory of Chukwuemeka Odumegwu Ojukwu University, Uli. The organisms used were obtained from urine samples of students and characterized using microbiological and biochemical tests. The phytochemical and proximate analysis of the seed extract of the plant was determined quantitatively using chemical and spectrophotometric methods. The inhibitory activity test was carried out using agar well diffusion method also tube dilution technique using double-fold serial dilution method was employed for assaying the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at various concentrations (31.25, 62.5, 125, 250 and 500 mg/ml). The phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, saponins, phenols, cyanogenic glycosides and steroids. The proximate content revealed that the seed contained more of moisture than fat and fibre. The ethanolic extract of Persea americana seed showed a pronounced activity (11.00 mm and 9.40 mm) against Escherichia coli and Staphylococcus aureus were significantly higher (P<0.05) than that of the aqueous extract (5.90 mm) which showed for only Escherichia coli. This activity differed significantly (P>0.05) from that of Ciprofloxacin (22.10 mm and 16.40 mm). The results of MIC for the ethanolic extract against E. coli and S. aureus were 500 mg/ml and 250 mg/ml respectively, while the MBC result was pronounced for only E. coli at 500 mg/ml. The spectra of antimicrobial activities displayed by the extracts could be attributed to the presence of these phytochemicals and signifies the potential of Persea americana as a source of therapeutic agent.
Escherichia coli, Staphylococcus aureus, Persea Americana Seed, MIC, MBC
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Umeaku Chinyelu Nkiru, Chris-Umeaku Chiamaka Ijeoma, Emmy-egbe Ifeyinwa Orsla, Ukoha Chinwe Clarice, Uzor Ugonwa Chiagoziem, Agbo Uchenna Juliet, Proximate, Phytochemical and Antibacterial Analysis of Persea americana Obtained from Nigeria, Journal of Diseases and Medicinal Plants. Vol. 4, No. 3, 2018, pp. 89-95. doi: 10.11648/j.jdmp.20180403.14
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Adebayo-tayo, B.C and Adegoke, A.A. (2008). Phytochemical and microbial screening of herbal remedies in Akwa Ibom State, South-Southern Nigeria, Journal of Medicinal Plants Research 2(11): 306–310.
Adegoke, A. A. and Adebyo-tayo, B. C. (2009). Antibacterial activity and Phytochemical analysis of leaf extracts of Lasienthera africanum. African Journal of Biotechnology 8 (1): 077–080.
Anon. New Delhi, India: CSIR, (2008). The useful plants of India. Retrieved from 11 May, 2017. pp 1 – 3.
Bibitha, B., Jisha, V.K., Salitha, C.V, Mohan, S. and Valsa, A.K. (2002). Antibacterial activity of different plants extracts. Short Communications. Indian Journal of Microbiology 42: 361–363.
Cheesbrough, M. (2000). District laboratory practice in tropical countries. Fourth edition. Cambridge University Printing Press, CB2 2RU, UK, 62-70.
Coe,F.L., Evan, A. and Worcester, E. (2005). Kidney Stone Disease. Journal of Chemical Investigations 115(10): 2598–2608.
Dabas, D., Elias, R.J., Lambert, J.D. and Ziegler, G.R. (2011). A colored avocado seed extract as a potential natural colorant. Journal of Food Science 76:1335–1341.
Farombi, E.O. (2003). African indigenous plant with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylacudiestic agents. African Journal of Biotechnology 2(12): 662–671.
Harborne, J.B. (1984). Methods of plant analysis. Phyochemical Methods 1–3.
Hung, T.M., Cuong, T.D., Dang, N.H., Zhu, S., Long, P.Q., Komatsu, K. and Min, B.S. (2011). Flavonoid, glycosides from Chromolaena odorata leaves and there in vitro cytotoxic activity. Chemical Pharmacy Bulletin 59 (1): 129–131.
Kunow, M.A. (2003). Plant Catalog: Appendix A. In: Kunow MA, ed., Maya medicine: traditional healing in Yucatan. University of New Mexico Press: Albuquerque; pp. 129.
Latha, S.P. and Kannabiran, K.(2006). Evaluation of antibacterial and phytochemical analysis of Trinobatum linu .Africa Journal of Biotechnology 5 (23): 2402–2404.
Mamidala, S.V.F., Matos, F.J.A., Leal-Cardoso, J.H. and Criddle, D.N. (2013). Relaxant effects of the essential oil of Ocimum gratissimum on isolated ileum of the guinea pig. Journal of Ethnopharmacology81: 1–4.
Morton, J.F. and Dowling, C.F.(1987). Avocado. In: Fruits of Warn Climate.:Winter Ville, N.C. pp.91–102.
Naveh, E., Werman, M.J., Sabo, E. and Neeman, I. (2002). Defatted avocado pulp reduces body weight and total heatic fat but increases plasma cholesterol in male rats fed diets with cholesterol. Journal of Nutrition 132(7):2015–2018.
Njume, G., Afoiayan, A.J., Clarke A.M. and Ndip, R.N. (2011). Crude ethanolic extracts of Garicinia kola seeds Heckel prolong the lag phase of Helicobacter pylori: inhibitory and bactericidal potential. Journal of Medicinal Food 14 (78): 822–827.
Nwachukwu, N.C., Orji, F.A..Iheukwuemere I.H. and Ekeleme, G.U. (2010). Antibiotic resistant environmental Listeria monocytogenes from anthropogenic lakes in Lokpu- Ukwu, Abia State of Nigeria. Australian Journal of Basic and Applied Sciences 4(7): 1571–1576.
Nwobu, A.W., Ezike, A.C., Nwafor, S.O., Okoli, C.O. and Enwerem, N.M. (2010). Evaluation of the antiasthmatic property of Asytasia gangetica leaf extracts. International Journal of Ethnopharmacology59: 284–290.
Obi, V.I. and Onuoha, C. (2000). Extraction and characterization method of plants and plant products. In: Biological and Agricultural Techniques Ogbule, J.N. and Ojiako, O.J. (edn.) Websmedia Publications, Owerri, pp. 271–286.
Okwu, D.E. and Josiah, C. (2006). Evaluation of the chemical composition of two Nigerian medicinal plants. African Journal of Biotechnology5(4):257–361.
Oleata, J.A., Schwartz, M. and Contreras, S. (2007). Use of Hass avocado (Pcrsea americana Mill.) seed as a processed product. In: VI World Avocado Congress (Actas VI Congreso Mundial delAguacatc): Chile, pp. 1–8.
Oyetayo, V.O. and Oyetayo, F.L. (2006). Phytochemical screening and antibacterial properties of siam weed: Chromolaena odorata leaf against aerobic isolates of wound. Journal Applied Environmental Sciences 2 (1): 7–11.
Ozolua, R.I., Amaka, O.N., Okpo, S.O. and Idogun, S.E. (2009). Acute and sub-acutetoxicological assessment of the aqueous seed extract of Persea Americana Mill (Lauraceae) in rats. African Journal of Traditional and Complementary Alternative Medicines 6:573–578.
Parekh, J., Karathia, N. and Chanda, S. (2006). Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegate L. Bark. African Journal of Biomedical Research 9: 53–56.
Prescott, L.M., Harvey, J.P. and Klein, D.A. (2005). Microbiology International Edition, McGraw-Hill, New York, pp. 601–627.
Soong, Y.J., Lee, D.Y., Kim, S.N., Lee, K.R., Lee, H.W., Han, J.W., Kan, D.W., Lee, H.Y. and Kim, Y.K. (2005). Apoptotic potential of sesquiterpene lactoraergolide through the inhibition of NF-B signaling Pentose Pathway. Journal of Pharmacology 57(2):1591–1597.
Soniya, M., Kuberan, T., Anitha, S. and Sankareswari, P. (2013). In vitro antibacterial activity of plant extracts against Gram positive and Gram negative pathogenic bacteria. International Journal of Microbiology and Immunology Resources 2(1): 001–005.
Taylor, R.S.L., Manandhar, N.P., Hudson, J.B. and Towers, G.H.N. (1995). Screening of selected medicinal plants of Nepal for antimicrobial activities. Journal of Ethno pharmacology 546: 153–159.
Umamaheswari, A., Shreevidya, R. and Apavna, N. (2008). In vitro antibacterial activity of Bongainvillea spectabilis leaves extracts, Advances in Biological Research 2: 1–5.
Umeaku, C.N. (2012). Comparing the Efficacy of Cassia alata Leaf Extract with Some Commercially Available Antifungal Agents.Abstract of International Congress for Human and Animal Mycology (ISHAM).Berlin, Germany.Abstract number A – 464-00652.Poster Presentation number pp 094.
Wafaa, A.A., Soumaya, S.A, Hatem, M.E. and Rehab, E.D. (2012). A trial to prevent Salmonella enteritidis infection in broiler chickens using Antigenous bacterin compared with probiotic preparation. Journal of Agricultural Science 4(5):91–108.
Wardlaw, G.M. and Kessel, M.W. (2002). Perspective in Nutrition. 5th edition, McGraw-Hill companies Inc., New York, USA, pp. 250–258.
Willey, J.M., Scherwood, L.M. and Woolverton, C. (2008). Prescott, Harley and Klein’s Microbiology, Seventh Edition McGraw-Hill companies, New York, pp. 113–116.
Yadav, R.N.S., and Munin, A. (2011). Phytochemical analysis of some medicinal plants. Journal of Phytology 3(12): 2319–7064.
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