Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia
Journal of Diseases and Medicinal Plants
Volume 3, Issue 5, October 2017, Pages: 88-96
Received: Oct. 6, 2017; Accepted: Oct. 28, 2017; Published: Nov. 30, 2017
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Authors
Gédéon Bongo, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo; Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania
Huruma Tuntufye, Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania
Koto-te-Nyiwa Ngbolua, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Joseph Malakalinga, Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania
Tshiama Claudine, Enseignement et Administration en Soins Infirmiers, Section Sciences Infirmières, Institut Supérieur des Techniques Médicales, Kinshasa, Democratic Republic of the Congo
Aaron Pambu, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Fabrice Mwanza, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Clément Mbadiko, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Gisèle Makengo, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Jeff Iteku, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Dorothée Tshilanda, Department of Chemistry, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Pius Mpiana, Department of Chemistry, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Théophile Mbemba, Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Rudovick Kazwala, Department of Veterinary Medicine and Public Health, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania
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Abstract
Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp. and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.
Keywords
Tuberculosis, Lowenstein Jensen, Anti-mycobacterial Activity, Phytochemicals, Medicinal Plants
To cite this article
Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, Aaron Pambu, Fabrice Mwanza, Clément Mbadiko, Gisèle Makengo, Jeff Iteku, Dorothée Tshilanda, Pius Mpiana, Théophile Mbemba, Rudovick Kazwala, Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia, Journal of Diseases and Medicinal Plants. Vol. 3, No. 5, 2017, pp. 88-96. doi: 10.11648/j.jdmp.20170305.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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