Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from Physalis peruviana L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats
Journal of Diseases and Medicinal Plants
Volume 2, Issue 6, December 2016, Pages: 67-73
Received: Jul. 10, 2016; Accepted: Jul. 20, 2016; Published: Nov. 22, 2016
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Authors
Felicien M. Kasali, Department of Pharmacy, Faculty of Medicine and Pharmacy, Official University of Bukavu, Bukavu, Bukavu-Democratic Republic of Congo; Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Charles Ntungwen Fokunang, Department of Pharmaco-Toxicology and pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Joseph Ngoupayo, Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Estella Tembe-Fokunang, Department of Pharmaco-Toxicology and pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Bathelemy Ngameni, Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Borgia Njinkio, Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Frederick Kechia, Department of Bacteriology, Virology, Parasitology and Infctious Diseases, Faculty of Medicines and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Justin N. Kadima, Department of Pharmacology, School of Medicine and Health Sciences, University of Rwanda, Butare, Rwanda
Tsague Marthe, Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Oyono Victor, Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Wilfred Mbacham, Department of Biochemistry, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon
Bonaventure Tchaleu Ngadjui, Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
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Abstract
The purpose of this study was to investigate the antidiabetic activity of extracts from leaves of Physalis peruviana L. used in the Eastern part of the Democratic Republic of the Congo used against diabetes. Different fractions with hexane, ethyl acetate and residue were obtained from the hydroalcoholic extract of Physalis peruviana leaves. The antidiabetic evaluation of hydroalcoholic and its fractions was evaluated in diabetic rats by a single administration of streptozotocin (50 mg/kg body weight) intravenously. The Reference group received glibenclamide (6.5 mg/kg body weight) and each test group received 100 mg/kg of body weight. Those groups were compared with a control group which received only a Tween 20 solution (1 ml per 100 g body weight). Serum biochemical profiles were evaluated by some blood markers including serum glucose, serum alanine transaminase (ALT), serum aspartate transaminase (AST) serum creatinine, total protein, triglyceride, total cholesterol, HDL-cholesterol and LDL-cholesterol. Antidiabetic profile evaluation showed no significant variation (P < 0.05) in blood glucose between groups after 28 days of treatment. There was no significant difference in the biochemical markers change including creatinine, ALT, AST, total protein, triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol. The hydroalcoholic extract from the leaves of Physalis peruviana L. and its fractions showed antidiabetic activity suggesting future detailed studies for new chemical entities lead drug discovery.
Keywords
Physalis peruviana, Antidiabetic, Hydroalcoholic Extract, Streptozotocin, Biochemical Parameters, Wistar Rats
To cite this article
Felicien M. Kasali, Charles Ntungwen Fokunang, Joseph Ngoupayo, Estella Tembe-Fokunang, Bathelemy Ngameni, Borgia Njinkio, Frederick Kechia, Justin N. Kadima, Tsague Marthe, Oyono Victor, Wilfred Mbacham, Bonaventure Tchaleu Ngadjui, Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from Physalis peruviana L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats, Journal of Diseases and Medicinal Plants. Vol. 2, No. 6, 2016, pp. 67-73. doi: 10.11648/j.jdmp.20160206.12
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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