Pectin-Chitosan Polyelectrolyte Complex Nanoparticles for Encapsulation and Controlled Release of Nisin
American Journal of Polymer Science and Technology
Volume 3, Issue 5, September 2017, Pages: 82-88
Received: Oct. 3, 2017;
Accepted: Oct. 19, 2017;
Published: Nov. 8, 2017
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Hui Wang, Department of Food Science and Technology, Hainan Tropical Ocean University, Sanya, China
Bo Yang, Department of Food Science and Technology, Hainan Tropical Ocean University, Sanya, China
Hongyuan Sun, Department of Food Science and Technology, Hainan Tropical Ocean University, Sanya, China
Nisin is a broad spectrum antimicrobial effective against Gram-positive bacteria. Antibacterial activity of Nisin is reduced when it is applied in food, due to binding with food matrix components. Encapsulation of Nisin in pectin-chitosan polyelectrolyte complex was prepared to protect Nisin from losing efficacy. Systematically, a number of parameters, pectin degree of esterification (DE), m (PE): m (CHI) mass ratio and solution pH were explored and their effect on the formation of stable polyelectrolyte nano complex colloid between pectin (PE) and chitosan (CS) was determined. Electrostatic interactions between carboxyl groups on pectin and amino groups on chitosan are confirmed by FTIR. The effects of DE of pectin on Nisin-loaded encapsulation properties were studied. Nanocapsules prepared by low methoxyl pectin (LPE) had higher encapsulation efficiency (EE) and loading capacity (LC) with smaller particle size, compared with those prepared by high methoxyl pectin (HPE). The largest EE is 65.9% when m (LPE): m (CHI) ratio was 20:15 and Nisin was 7 mg. Increasing amount of Nisin had a tendency to form nanocapsules with lower EE and higher LC and particle size. Release profile of Nisin from nanocapsules was affected by pH, more amounts of Nisin released at pH 3 than at pH 6. Encapsulated Nisin showed more active antibacterial activities against S. aureus than free Nisin. Encapsulation offers great promise to improve antibacterial effectiveness of Nisin.
Pectin-Chitosan Polyelectrolyte Complex Nanoparticles for Encapsulation and Controlled Release of Nisin, American Journal of Polymer Science and Technology.
Vol. 3, No. 5,
2017, pp. 82-88.
Zhou H, Fang J, Tian Y, et al. Mechanisms of nisin resistance in Gram-positive bacteria [J]. Annals of microbiology, 2014, 64 (2): 413-420.
Punyauppa-path S, Phumkhachorn P, Rattanachaikunsopon P. Nisin: Production and mechanism of antimicrobial action [J]. International Journal of Current Research and Review, 2015, 7 (2): 47.
Bhatti M, Veeramachaneni A, Shelef L A. Factors affecting the antilisterial effects of nisin in milk [J]. International journal of food microbiology, 2004, 97 (2): 215-219.
Aasen I M, Markussen S, Møretrø T, et al. Interactions of the bacteriocins sakacin P and nisin with food constituents [J]. International journal of food microbiology, 2003, 87 (1): 35-43.
Prombutara P, Kulwatthanasal Y, Supaka N, et al. Production of nisin-loaded solid lipid nanoparticles for sustained antimicrobial activity [J]. Food Control, 2012, 24 (1): 184-190.
Hosseini S M, Hosseini H, Mohammadifar M A, et al. Preparation and characterization of alginate and alginate-resistant starch microparticles containing nisin [J]. Carbohydrate polymers, 2014, 103: 573-580.
da Silva Malheiros P, Daroit D J, da Silveira N P, et al. Effect of nanovesicle-encapsulated nisin on growth of Listeria monocytogenes in milk [J]. Food Microbiology, 2010, 27 (1): 175-178.
da Silva Malheiros P, Sant'Anna V, de Souza Barbosa M, et al. Effect of liposome-encapsulated nisin and bacteriocin-like substance P34 on Listeria monocytogenes growth in Minas frescal cheese [J]. International journal of food microbiology, 2012, 156 (3): 272-277.
Taylor T, Bruce B D, Weiss J, et al. Listeria monocytogenes and Escherichia coli O157:H7 inhibition in vitro by liposome-encapsulated nisin and ethylene diaminetetraacetic acid [J]. Journal of food safety, 2008, 28 (2): 183-197.
Imran M, Revol-Junelles A M, Paris C, et al. Liposomal nanodelivery systems using soy and marine lecithin to encapsulate food biopreservative nisin [J]. LWT-Food Science and Technology, 2015, 62 (1): 341-349.
Xiao D, Davidson P M, Zhong Q. Release and antilisterial properties of nisin from zein capsules spray-dried at different temperatures [J]. LWT-Food Science and Technology, 2011, 44 (10): 1977-1985.
Chen H, Zhong Q. A novel method of preparing stable zein nanoparticle dispersions for encapsulation of peppermint oil [J]. Food Hydrocolloids, 2015, 43: 593-602.
Guiga W, Swesi Y, Galland S, et al. Innovative multilayer antimicrobial films made with Nisaplin® or nisin and cellulosic ethers: Physico-chemical characterization, bioactivity and nisin desorption kinetics [J]. Innovative food science & emerging technologies, 2010, 11 (2): 352-360.
Narsaiah K, Jha S N, Wilson R A, et al. Optimizing microencapsulation of nisin with sodium alginate and guar gum [J]. Journal of food science and technology, 2014, 51 (12): 4054-4059.
Ji S, Lu J, Liu Z, et al. Dynamic encapsulation of hydrophilic nisin in hydrophobic poly (lactic acid) particles with controlled morphology by a single emulsion process [J]. Journal of colloid and interface science, 2014, 423: 85-93.
Gharsallaoui A, Joly C, Oulahal N, et al. Nisin as a food preservative: Part 2: Antimicrobial polymer materials containing nisin[J]. Critical reviews in food science and nutrition, 2016, 56 (8): 1275-1289.
Chandrasekar V, Coupland J N, Anantheswaran R C. Characterization of nisin containing chitosan-alginate microparticles [J]. Food Hydrocolloids, 2017, 69: 301-307.
Chopra M, Kaur P, Bernela M, et al. Surfactant assisted nisin loaded chitosan-carageenan nanocapsule synthesis for controlling food pathogens [J]. Food Control, 2014, 37: 158-164.
Bernela M, Kaur P, Chopra M, et al. Synthesis, characterization of nisin loaded alginate–chitosan–pluronic composite nanoparticles and evaluation against microbes [J]. LWT-Food Science and Technology, 2014, 59 (2): 1093-1099.
Van der Gucht J, Spruijt E, Lemmers M, et al. Polyelectrolyte complexes: bulk phases and colloidal systems [J]. Journal of colloid and interface science, 2011, 361 (2): 407-422.
Park M R, Seo B B, Song S C. Dual ionic interaction system based on polyelectrolyte complex and ionic, injectable, and thermosensitive hydrogel for sustained release of human growth hormone [J]. Biomaterials, 2013, 34 (4): 1327-1336.
Tsai R Y, Chen P W, Kuo T Y, et al. Chitosan/pectin/gum Arabic polyelectrolyte complex: Process-dependent appearance, microstructure analysis and its application [J]. Carbohydrate polymers, 2014, 101: 752-759.
Morris G A, Castile J, Smith A, et al. Macromolecular conformation of chitosan in dilute solution: A new global hydrodynamic approach [J]. Carbohydrate Polymers, 2009, 76 (4): 616-621.
Wai W W, Alkarkhi A F M, Easa A M. Effect of extraction conditions on yield and degree of esterification of durian rind pectin: An experimental design [J]. Food and bioproducts processing, 2010, 88 (2): 209-214.
Fellah A, Anjukandi P, Waterland M R, et al. Determining the degree of methylesterification of pectin by ATR/FT-IR: Methodology optimisation and comparison with theoretical calculations [J]. Carbohydrate polymers, 2009, 78 (4): 847-853.
Morris G A, Kök S M, Harding S E, et al. Polysaccharide drug delivery systems based on pectin and chitosan [J]. Biotechnology and Genetic Engineering Reviews, 2010, 27 (1): 257-284.
Maestrelli F, Cirri M, Mennini N, et al. Influence of cross-linking agent type and chitosan content on the performance of pectinate-chitosan beads aimed for colon-specific drug delivery [J]. Drug development and industrial pharmacy, 2012, 38 (9): 1142-1151.
Birch N P, Schiffman J D. Characterization of self-assembled polyelectrolyte complex nanoparticles formed from chitosan and pectin [J]. Langmuir, 2014, 30 (12): 3441-3447.
Davidenko N, Blanco M D, Peniche C, et al. Effects of different parameters on the characteristics of chitosan–poly (acrylic acid) nanoparticles obtained by the method of coacervation [J]. Journal of Applied Polymer Science, 2009, 111 (5): 2362-2371.
Bigucci F, Luppi B, Cerchiara T, et al. Chitosan/pectin polyelectrolyte complexes: selection of suitable preparative conditions for colon-specific delivery of vancomycin [J]. European journal of pharmaceutical sciences, 2008, 35 (5): 435-441.
Joye I J, McClements D J. Biopolymer-based nanoparticles and microparticles: fabrication, characterization, and application [J]. Current Opinion in Colloid & Interface Science, 2014, 19 (5): 417-427.
Ramasamy T, Tran T H, Cho H J, et al. Chitosan-based polyelectrolyte complexes as potential nanoparticulate carriers: physicochemical and biological characterization[J]. Pharmaceutical research, 2014, 31 (5): 1302.
Krivorotova T, Cirkovas A, Maciulyte S, et al. Nisin-loaded pectin nanoparticles for food preservation[J]. Food Hydrocolloids, 2016, 54: 49-56.
Marudova M, MacDougall A J, Ring S G. Pectin–chitosan interactions and gel formation [J]. Carbohydrate research, 2004, 339 (11): 1933-1939.
Coimbra P, Ferreira P, De Sousa H C, et al. Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications [J]. International journal of biological macromolecules, 2011, 48 (1): 112-118.
Patravale V B, Kulkarni R M. Nanosuspensions: a promising drug delivery strategy [J]. Journal of pharmacy and pharmacology, 2004, 56 (7): 827-840.