Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography
American Journal of Polymer Science and Technology
Volume 2, Issue 2, November 2016, Pages: 20-27
Received: Aug. 27, 2016;
Accepted: Sep. 23, 2016;
Published: Oct. 14, 2016
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Doudou Zhang, College of Pharmaceutical Sciences, Hebei University, Baoding, China
Beijiao Cui, College of Pharmaceutical Sciences, Hebei University, Baoding, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China
Xiaoya Jiang, College of Pharmaceutical Sciences, Hebei University, Baoding, China
Ligai Bai, College of Pharmaceutical Sciences, Hebei University, Baoding, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China
Haiyan Liu, College of Pharmaceutical Sciences, Hebei University, Baoding, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China
In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl2 as catalyst, and CCl4 as initiator. Different monolithic columns were obtained by changing the conditions or columns sizes. These columns were used as the stationary phases of high performance liquid chromatography to investigate the factor effecting on the column efficiency. The effects of column diameter (D), pore size (d), porosity (Ö), and linear velocity of the mobile phase (u) on the performance of polymer monolithic columns were studied and the results certified that the plug-like flow in the column was one of the key factors shaping high performance. Furthermore, a structural hydrodynamic model was introduced to investigate the effects of polymer morphology on the performance of liquid chromatography with monolithic columns.
Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography, American Journal of Polymer Science and Technology.
Vol. 2, No. 2,
2016, pp. 20-27.
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