Effect of Aged Garlic Extract on Prodromal Phase After Acute Radiation Syndrome
Nuclear Science
Volume 1, Issue 1, December 2016, Pages: 6-17
Received: Oct. 24, 2016; Accepted: Nov. 23, 2016; Published: Dec. 27, 2016
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Kouam Foubi Brice Bertrand, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Chuisseu Djamen Dieudonné Pascal, Faculty of Health Sciences, Université des Montagnes, Bangangté, Bangangté, Cameroon
Dzeufiet Djomeni Paul Désiré, Faculty of Sciences, University of Yaoundé I, Yaoundé, Cameroon
Samba Ngano Odette, Faculty of Sciences, University of Dschang, Dschang, Cameroon
Mouelle Sone, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Ongolo Zogo Pierre, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Dong à Zok Faustin, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Yomi Jean, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Juimo Alain George, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Nko’o Amvene Samuel, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Tiedeu Alain Bertin, National Advanced School of Engineering, University of Yaoundé I, Yaoundé, Cameroon
Gonsu Fotsin Joseph, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
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Acute radiation syndrome results in 4 four phases among which the prodromal one characterized by malaise, diarrhea, vomiting, and anorexia. It last a few hours, but from irradiation to the 7th day after, some other signs (unspecified, mild, moderate or severe) are observed. Thus, the aim of this study was to assess if Aged garlic extract (AGE) could offer radioprotection by preventing signs of prodromal phase after acute radiation syndrome. Knowing, AGE is a natural product containing different compounds with antioxidant activity and possessing several physiological activities in experimental animals. Eight groups, five healthy male rats each were used (20 irradiated and 20 Sham Irradiated), among which some were receiving via gavages distilled water, the others AGE at different doses (25 mg/kg and 50 mg/kg) and the rest vitamin E + Alpha Lipoïc Acid. The signs observed and evaluated (behavioral changes, salivation, state stool, water and food intake, weight loss, relative weight of organs, fatigue/weakness, temperature and bleeding) revealed a significant change in irradiated groups although AGE-pretreated rats prior and after irradiation revealed significant amelioration in radiation-induced disturbances. Therefore AGE can be considered having radioprotective effects against radiation-induced changes in prodromal phase signs.
Irradiation, Radiation Syndrome, Prodromal Phase, AGE, Rats
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Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Mouelle Sone, Ongolo Zogo Pierre, Dong à Zok Faustin, Yomi Jean, Juimo Alain George, Nko’o Amvene Samuel, Tiedeu Alain Bertin, Gonsu Fotsin Joseph, Effect of Aged Garlic Extract on Prodromal Phase After Acute Radiation Syndrome, Nuclear Science. Vol. 1, No. 1, 2016, pp. 6-17. doi: 10.11648/j.ns.20160101.12
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Ganesh C. Jagetia. Radioprotective potential of plants and Herbs against the effects of Ionizing Radiation. J. Clin. Biochem. Nutr. 2007; 40: 74-81.
Yamini K, Gopal V. Natural radioprotective agents against Ionizing Radiation - An Overview. Int. J. Radiat. Biol. 2010; 2 (2): 1421-426.
Parihar V, Prabhakar K, Veerapur P, Kumar M, ReddyY, Joshi R, et al Effect of radiation-induced cytotoxicity is in Swiss albino mice, Mutat Res. 2006; 611: 1-2, 9-16.
DECRET 2002/250 du 31 OCTOBRE 2002 portant création, organisation et fonctionnement de l’Agence Nationale de Radioprotection. Consultable en ligne sur: http://opencamer.blogspot.com/2013/09/decret-2002250-du-31-octobre-2002.html
Waselenko JK, MacVittie TJ, Blakely WF, Pesik N, Wiley AL, Dickerson WE, Tsu H, Confer DL, Coleman CN, Seed T, Lowry P, Armitage JO, Dainiak N. Medical Management of the Acute Radiation Syndrome: Recommendations of the Strategic National Stockpile Radiation Working Group. Ann Intern Med. 2004; 140: 1037-1051.
Cerveny T. J., MacVittie T. J., Young R. W. Acute radiation syndrome in humans. Textbook of Military Medecine - Medical Consequences of Nuclear Warfare, Armed Forces Radiobiology Research Institute, Bethesda, Maryland, 1989; 17-36.
Donnelly EH, Nemhauser JB, Smith JM. "Acute radiation syndrome: assessment and management". South. Med. J. 2010; 103 (6): 541–6.
Armin Ansari. Radiation Threats and Your Safety: A Guide to Preparation and Response for Professionals and Community. 2009; p. 110.
Tanaka T. Cancer chemoprevention by natural products. Oncol. Rep. 1994; 1: 1139–1155.
Kong Ah-Ng T., Yu R., Chen C., Mandlekar S. and Primiano T., Signal transduction events elicited by natural products: Role of MAPK and caspase pathways in homeostatic response and induction of apoptosis. Arch. Pharm. Res. 2000; 23: 1–16.
Ahmed R. S., Seth V. and Banerjee B. D. Influence of dietary ginger (Zingiber officinales Rosc) on antioxidant defense system in rat: Comparison with ascorbic acid. Indian J. Exp. Biol. 2000; 38: 604–606.
Amagase H., Petesch B. L., Matsuura H., Kasuga S., and Itakura Y. “Intake of garlic and its bioactive components,” Journal of Nutrition. 2001; 131 (3): 955–962.
Balamash K, Albar O, Wang Q and Ahmed N: Effect of Kyolic® aged garlic extract on glycaemia, lipidaemia and oxidative stress in patients with type 2 diabetes mellitus. Journal of Diabetes Research and Clinical Metabolism. 2012; 1: 18.
EEC: Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Communities 1986; L358: 1–29.
Medical Management of Radiological Casualties-Handbook. 2nd ed. Bethesda: Armed Forces Radiology Research Institute; 2003.
Walker RI, Cerveny RJ, eds. Medical Consequences of Nuclear Warfare. Falls Church, VA: Office of the Surgeon General; 1989. Available at www.afrri.usuhs.mil.
Institute of Medicine/National Research Council. Potential Radiation Exposure in Military Operations: Protecting the Soldier Before, During and After. Committee on Battlefield Radiation Exposure Criteria. Washington, DC: National Academy Pr; 1999.
Data adapted by Radiation Emergency Medical management (REMM) from NATO Handbook on the Medical Aspects of NBC Defensive Operations AMedP-6(B), Chapter 6, General Medical Effects of Nuclear Weapons: Diagnosis, Treatment, and Prognosis, 1 February, 1996.
Gatsing et al. Toxological evaluation of the aqueous extract of Allium sativum L. on laboratory mice and rats. Cameroon Journal Experimental Biology. 2005; 1 (1): 39-45.
Mawdo Ngom. Contribution à l’étude des effets androgéniques des extraits aqueux des racines entières de nauclea latifolia sm. Étude expérimentale chez le rat. Mémoire, Faculté de Médecine, Pharmacie et d’Odonto-Stomatologie, 2010: Dakar.
Kameni Poumeni M. Evaluation aphrodisiac effects of aqueous extract of the flowers of lotus nymphaea on reproductive function of normal male rats with type 1 diabetes. Master thesis, animal physiology option. University of Yaounde I. Facebook sciences. 2011; p. 86.
Plaznik A., Stefanski R., Palejko W., Kotawski W. The role of accumbens GABA-B receptors in the regulation of rat behaviour. Neuroscience Research Com. 1993; 12: 23–30.
Koen. Température. [En ligne]. Disponible sur: http://www.ratzine.fr/: (Consulté le 23/09/2016).
Ganesh Chandra Jagetia, Manjeshwar Shrinath Baliga, Ponemone Venkatessh, and Jagadish N. Ulloor. Influence of Ginger Rhizome (Zingiber officinale Rose) on survival, Glutathione and Lipid Peroxidation in Mice after Whole-Body Exposure to Gamma Radiation. Radiation Research. 2003; 160 (5): 584-592. DOI: http://dx.doi.org/10.1667/RR3057
Koropatnick, J.; Leibbrand, M. and cherian, M. G. Organspecific metallothionein induction in mice by X-irradiation. Rad Res. 1989; 119 (2): 356-65.
Kondoh, M. and Sato, M. Protective stress responsive protein: metallothionein. Nippon. Eiseigaku. Zasshi. Jap. J. Hygiene. 2002; 56 (4): 634-40.
Uma Devi and A. Ganasoundari, Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum. Indian J. Exp. Biol. 1995; 33: 205-209.
Jagetia G. C. and Baliga M. S. Influence of the leaf extract of Menthaarvensis Linn. (mint) on the survival of mice exposed to different doses of gamma radiation. Strahlenther. Onkol. 2002; 178: 91-98.
Bond V. P., Fliedner T. M. and Archambeau J. O. Mammalian Radiation Lethality. Academic Press, New York, 1965.
Alain, Patrick, Laurent Martigne. Neuropathologie radio-induite: des effets précoces aux séquelles tardives. Etudes comportementales et métaboliques chez le Rat après irradiation globale sublétale. Neurons and Cognition. Université Joseph-Fourier - Grenoble I, 2010.
Hall E. J. Radiobiology for the radiologist, 4th ed. J. B. Lippincott, Philadelphia, 1994.
Rouhaniwadeh N. Etude radiobiologique de l’activité radioprotectrice d’un composé naturel iranien: le «SHIR-KHECHT». Rapport français Commissariat à l’Energie Atomique (CEA) - R3412, 1968.
Amagase H. Intake of garlic and its components. Nutritional and Health Benefits of Garlic as a Supplement Conference, Newport Beach, CA. 1998; p. 4.
Lawson LD, Ransom D, Hughs B. Inhibition of whole blood platelet aggregation by compounds in garlic clove extracts and commercial garlic products. Thromb 1992. Res. 65: p. 141–56.
Freeman F, Kodera Y. Garlic chemistry: stability of S-(2-propenyl) 2-propene-1-sulfinothioate (allicin) in blood, solvents and simulated physiological fluids. J. Agric. FoodChem. 1995; 43: 2332–338.
Lehningher L. A. Principles of biochemistry. New York: Worth Publishers, Inc. 1011p.
Eisenhauer L., Nichols W. L., Spencer T. R., And Bergan W. F. Clinical Pharmacology and nursing Management. Philadelphia, New York. Lippincott: 779-809.
Radiation Emergency Medical management (REMM) from NATO Handbook on the Medical Aspects of NBC Defensive Operations AMedP-6(B), Chapter 6, General Medical Effects of Nuclear Weapons: Diagnosis, Treatment, and Prognosis, 1 February, 1996, available on www.remm.nlm.gov.
Monique Pressac. Conférences des Universités. Laboratoire d’Endocrinologie de Pharmacie de l’Observatoire PARIS 2004/ 2005.
Descat Fleur. Hématologie du rat: hémogramme et Myélogramme. Thesis, National school of veterinary Toulouse III, 2002: Toulouse.
Hall EJ. Acute effects of total-body irradiation. In: Hall EJ. Radiobiology for the Radiologist. 5th ed. Philadelphia: Lippincott Williams & Wilkins; 2000: 124-35.
Dharmendra K Maura, Thomas P A Devasagayam and Cherpall Krishnan K Nair. Some novel approaches for radioprotection and the beneficial effect of natural products. Indian journal of experimental Biolog 2006; 44: 93-114.
Abd-El Allatif, A. and K. Ebraheem. Studies on the effects of Hibiscus subdariffa, Allium sativum and Negella sativa on some bacterial isolates of chickens. Fac. Vet. Med. Assute University Egypt. 1996; 17: 245-51.
Borzelleca J F. A proposed model for safety assessment of macronutrient substitutes. Regul. Toxicol. Pharmacol. 1996; 23: 15–18.
Block E.. “The chemistry of garlic and onions”. Scientific American. 1985; 252 (3): 114–119,
Anonymous. Monographs on the medicinal uses of plants. Exeter: European Scientific Cooperative on Phytotherapy, 1997.
Blania G, Spangenberg B. Formation of allicin from dried garlic (Allium sativum): a simple HPTLC method for simultaneous determination of allicin and ajoene in dried garlic and garlic preparations. Planta Med. 1991; 57: 371-75.
Bradley P. British herbal compendium: a handbook of scientific information on widely used plant drugs /published by the British Herbal Medicine Association and produced by its Scientific Committee. Bournemouth, Dorset: The Association, 1992.
Lawson L, Wang Z, Hughes B. Identification and HPLC quantitation of the sulfides and dialkyl thiosulfinates in commercial garlic products. Planta Med. 1991; 57: 363-70.
Miething H. HPLC-Analysis of the volatile oil of garlic bulbs. Phytother Res. 1988; 2: 149-51.
Ip C, Lisk DJ. Bioavailability of selenium from selenium-enriched garlic. Nutr Cancer. 1993; 20: 129-37.
Ip C, Lisk D. Efficacy of cancer prevention by high-selenium garlic is primarily dependent on the action of selenium. Carcinogenesis. 1995; 16: 2649-652.
Ip C, Lisk D, Thompson HJ. Selenium-enriched garlic inhibits the early stage but not the late stage of mammary carcinogenesis. Carcinogenesis. 1996; 17: 1979-982.
Ip C, Lisk D. The attributes of selenium-enriched garlic in cancer prevention. Adv Exp Med Biol. 1996; 401: 179-87
Ip C, Lisk D. Modulation of phase I and phase II xenobiotic-metabolizing enzymes by selenium-enriched garlic in rats. Nutr Cancer. 1997; 28: 184-8.
Duke J. Handbook of Phytochemical Constituents of GRAS herbs and other economic Plants. London: CRC Press 1992.
Couturier P, Bousquet J. Occupational allergy secondary inhalation of garlic dust. J Allergy Clin Immunol. 1982; 70: 145.
Kaplan B, Schewach-Millet M, Yorav S. Factitial dermatitis induced by application of garlic. Int J Dermatol. 1990; 29: 75-6.
Moriguchi T, Saito H, Nishiyama N. Anti-aging effect of aged garlic extract in the inbred brain atrophy mouse model. Clin. Exp. Pharmacol. Physiol. 1997; 24: 235–42.
Encyclopédie du rat domestique. Température corporelle. [En ligne]. Disponible sur: http://www. ratoupédia.org/: (Consulté le 15/05/2016).
Bauck, L. Basic anatomy, physiology, husbandry, and clinical techniques. Part I.- In Hillyer, Quesenberry- Ferrets, Rabbits and Rodents. Clinical Medecine and Surgery.-Saunders- 1997- Chap 26, 291-297.
Harkness, J. E., Wagner, J. E.-The biology and medecine of Rabbits and Rodents- 4 Edition- Philadelphie: Lea and Febiger. 1994; pages 65-73, 93-96, 130-136.
Schull WJ. Effects of Atomic Radiation: A Half-Century of Studies from Hiroshima and Nagasaki. New York: J Wiley; 1996.
Barlow Y. T lymphocytes and immunosuppression in the burned patient: a review. Burns. 1994; 20: 487-90. [PMID: 7880410]
Maldonado MD, Venturoli A, Franco A, Nunez-Roldan A. Specific changes in peripheral blood lymphocyte phenotype from burn patients. Probable origin of the thermal injury-related lymphocytopenia. Burns. 1991; 17: 188-92. [PMID: 1892548]
Mistry S, Mistry NP, Arora S, Antia NH. Cellular immune response following thermal injury in human patients. Burns Incl Therm Inj. 1986; 12: 318- 24. [PMID: 2942227]
Cheadle WG, Pemberton RM, Robinson D, Livingston DH, Rodriguez JL, Polk HC Jr. Lymphocyte subset responses to trauma and sepsis. J Trauma. 1993; 35: 844-9. [PMID: 8263980]
Baranov AE, Guskova AK, Nadejina NM, Nugis VY. Chernobyl experience: biological indicators of exposure to ionizing radiation. Stem Cells. 1995; 13: 69-77. [PMID: 7488970]
Hrapkiewicz K., Medina L., Holmes D. D.-Clinical medicine of small mammals and primates-2nd Edition- 1998- Chap 2, Rats, 31-39.
Wolfensohn, S., Lloyd M. Handbook of laboratory animal: Management and Welfare -2nd Edition- 1998, section 2, 179-184.
Casarett, A. P. (Effect of radiation on blood cells. In: Radiation Biology. Prentice-Hall, Inc., Englewood Cliffs, New Gersy. 1968; 3: 125.
Kojima, N. and Okaw, J. Prospective analysis of radiation induced cytopenia. Biotherapy. 1999; 13 (5): 462.
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