Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene
World Journal of Public Health
Volume 2, Issue 1, March 2017, Pages: 38-50
Received: Nov. 4, 2016; Accepted: Nov. 24, 2016; Published: Jan. 7, 2017
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Authors
Yousif Elhassaneen, Nutrition and Food Science Department, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt
Sherif Ragab, Nutrition and Food Science Department, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt
Riham Badawy, Nutrition and Food Science Department, Faculty of Home Economics, Minoufiya University, Shebin El-Kom, Egypt
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Abstract
In this study, we evaluate the antioxidant effect and total phenolics content of four selected plant parts [prickly pear peel (PPP), turmeric rhizomes (TR), red onion skin (ROS) and eggplant peel (EP)] methanolic extracts and their potential role in improving the liver disorders induced by an ubiquitous food pollutant i.e. benzo(a)pyrene (BP). The selected plant parts methanolic extracts showed considerable differences in antioxidant activity (AA=57.51 to 90.73 %) and total phenolics (11.29 to 211.45 mg GAE. g -1). When all selected plant parts methanolic extracts were included in the statistical analysis, there was a positive and highly significant (r2=0.597-0.919, p≤ 0.05) relationship between total phenolics and antioxidant activity. Also, the antioxidant potential of those methanolic extracts against BP was also studied in vitro in a model using Catfish liver cells in homogenate culture. BP induced many metabolic disorders and oxidative stress in fish liver homogenate namely a significant decrease in reduced glutathione (GSH) and albumin (Alb) content, and increase in malondialdehyde (MDA) formation after 24 hours of culture. Co-treatment of liver homogenate with BP and the tested selected plant parts extracts as well as their mixture by concentration 0.75% exhibited some protection effects through decreasing the rates of all those metabolic disorders and oxidative stress. That decreasing rates in different adverse effects was depending on the type of the plant parts applied. The highest therapeutic effect was recorded for the mixture of the selected plant parts extracts (PPP+TR+ROS+EP by equal amounts) followed by ROS, EP, TR and PPP, respectively. This study indicates that the compounds present in those selected extracts contain interesting bioactivities which improve many adverse effects i.e. metabolic disorders and oxidative stress in liver cells induced by BP.
Keywords
Cat Liver Homogenate, Total Phenolics, Metabolic Disorders, Oxidative Stress, Onion Skin, Eggplant Peel, Prickly Pear Peel
To cite this article
Yousif Elhassaneen, Sherif Ragab, Riham Badawy, Antioxidant Activity of Methanol Extracts From Various Plant Parts and Their Potential Roles in Protecting the Liver Disorders Induced by Benzo(a)pyrene, World Journal of Public Health. Vol. 2, No. 1, 2017, pp. 38-50. doi: 10.11648/j.wjph.20170201.15
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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